Optimized protocol for conditioned place avoidance learning in juvenile zebrafish

Palumbo, Fabrizio; Serneels, Bram; Yaksi, Emre

doi:10.1016/j.xpro.2021.100465

Cited by 2 publications

(3 citation statements)

References 26 publications

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“…To investigate ongoing habenular activity, we performed volumetric two-photon calcium imaging across the entire habenula of juvenile Tg(elavl3:GCaMP6s) zebrafish, 29 , 55 , 56 , 57 expressing GCaMP6s pan-neuronally. In all our experiments, we used 3- to 4-week-old zebrafish 58 that were shown to exhibit complex behaviors such as learning 44 , 59 , 60 , 61 and social interactions, 62 , 63 which are mediated by habenular circuits. 36 , 41 , 42 , 44 , 46 We observed structured ongoing activity across the entire habenula ( Figure 1 A), in line with earlier studies focusing on the dHb.…”

Section: Resultsmentioning

confidence: 99%

“… 1 , 44 , 46 , 82 Discovering how the ongoing activity of these networks adapts during behavioral plasticity and learning will require well-established behavioral assays and simultaneous imaging of forebrain and habenular activity in juvenile zebrafish that can perform cognitively demanding learning tasks. 44 , 59 , 60 , 61 , 62 Moreover, given the multi-sensory responses of habenular circuits, 29 it will also be interesting to test whether such interactions between sensory stimuli and ongoing brain activity generalize to other sensory modalities (e.g., vision, auditory) in future studies.…”

Section: Discussionmentioning

confidence: 99%

“…Future studies of molecular profiling, anatomical reconstructions, and neural connectivity of forebrain neurons will be important to further support our calcium imaging-based functional clustering and connectivity measures. Given the ability of juvenile zebrafish to perform cognitively demanding behaviors such as social interactions 62 , 63 and learning, 44 , 59 , 60 , 61 while allowing non-invasive functional imaging, we hope that combinations of these approaches will shed further light onto the function and connectivity of forebrain networks during these behaviors.…”

Section: Discussionmentioning

confidence: 99%

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Ongoing habenular activity is driven by forebrain networks and modulated by olfactory stimuli

et al. 2021

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Summary Ongoing neural activity, which represents internal brain states, is constantly modulated by the sensory information that is generated by the environment. In this study, we show that the habenular circuits act as a major brain hub integrating the structured ongoing activity of the limbic forebrain circuitry and the olfactory information. We demonstrate that ancestral homologs of amygdala and hippocampus in zebrafish forebrain are the major drivers of ongoing habenular activity. We also reveal that odor stimuli can modulate the activity of specific habenular neurons that are driven by this forebrain circuitry. Our results highlight a major role for the olfactory system in regulating the ongoing activity of the habenula and the forebrain, thereby altering brain’s internal states.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Ongoing habenular activity is driven by forebrain networks and modulated by olfactory stimuli

et al. 2021

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Inhibition mediated by group III mGluRs regulates habenula activity and defensive behaviors

Ostenrath,

Faturos,

Çiftci Çobanoğlu

et al. 2024

Preprint

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Inhibition contributes to various brain computations from sensory motor transformations to cognitive operations. While most studies on inhibition focus on GABA, the main excitatory neurotransmitter of the brain, glutamate, can also elicit inhibition via metabotropic glutamate receptors (mGluRs). The function of mGluR-mediated inhibition remains largely elusive. Here, we investigated the role of group III mGluR-dependent inhibition in the habenula. This primarily glutamatergic and conserved forebrain region acts as a hub between multiple forebrain inputs and neuromodulatory mid- and hindbrain targets that regulate adaptive behaviors. We showed that both zebrafish and mice habenula express group III mGluRs. We identified that group III mGluRs regulate the membrane potential and calcium activity of zebrafish dorsal habenula. Pharmacological and genetic perturbation of group III mGluRs increased sensory-evoked excitation and reduced selectivity of habenular neurons to different sensory modalities. We also observed that inhibition is the main channel of communication between primarily glutamatergic habenula neurons. Blocking group III mGluRs reduced inhibition within habenula and increased correlations during spontaneous activity. In line with such inhibition within habenula, we identified that multi-sensory information is integrated mainly through competition and suppression across habenular neurons, which in part relies on group III mGluRs. Finally, genetic perturbation of a habenula-specific group III mGluR, mGluR6a, amplified neural responses and defensive behaviors evoked by sensory stimulation and environmental changes. Altogether, our results revealed that mGluR driven inhibition is essential in encoding, integration, and communication of information between Hb neurons, ultimately playing a critical role in regulating defensive and adaptive behaviors.

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Optimized protocol for conditioned place avoidance learning in juvenile zebrafish

Cited by 2 publications

References 26 publications

Ongoing habenular activity is driven by forebrain networks and modulated by olfactory stimuli

Ongoing habenular activity is driven by forebrain networks and modulated by olfactory stimuli

Inhibition mediated by group III mGluRs regulates habenula activity and defensive behaviors

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