Optimized slice-selective 1H NMR experiments combined with highly accurate quantitative 13C NMR using an internal reference method

Coherence Transfer by Radiation Damping Combined with J‐Couplings in 2D Correlation Spectroscopy at High‐Field Solution NMR

“…6,8 Radiation damping is also an ongoing problem in biological NMR applications, where the solvent peak is usually much larger than others. 12,13 In the past, this effect has been considered only when dealing with the signal of concentrated spins, such as for solvents. [9][10][11] However, it may be constructively used to suppress the water signal in some cases.…”

“…[2][3][4][5] This effect is quite dramatic for concentrated protons (e.g., in protonated solvents) and in most modern high-field NMR spectrometers. 12,13 In the past, this effect has been considered only when dealing with the signal of concentrated spins, such as for solvents. 6,7 The theoretical framework describing this effect has tremendously progressed over the last couple of decades.…”

“…Radiation damping has also a soft‐pulse effect that turns the corresponding spin into its equilibrium state, affecting the spin relaxation. Therefore, the suppression of the radiation damping effect is crucial to achieving high precision in quantitative NMR (qNMR) spectroscopy, a technique that has recently received much attention …”

Spatially Resolved Anisotropic Natural Abundance Deuterium 2D-NMR Spectroscopy Using Bimesophasic Lyotropic Chiral Systems

“…This avoids phase distortions that might occur from residual quadrupolar coupling modulation during the application of selective pulses. The thickness Δz and the position z 0 of the slice to be saturated are defined by the bandwidth (BW) and the offset (Spoff) of the selective pulse and the amplitude of the gradient (G) along z-axis. , Following the OVS block, any 1D or 2D experiment can be implemented, as depicted in Figure . Combined together, we can selectively record the 2 H or NAD 1D or 2D spectra corresponding to the upper and lower phase of the sample (see the SI).…”

“…For performing spatially resolved NMR experiments on bimesophasic samples, we adopted the selective saturation of volumes surrounding the region of interest, known as Outer Volume Suppression (OVS), that relies on the concurrent use of magnetic field gradients and selective pulses to saturate the targeted sample region. 20,21 Applied to 2 H NMR in anisotropic samples, the "OVS" approach is advantageous because this suppression block is easy to implement in any nD NMR pulse sequence, as it is generally performed during the experiment preparation period.…”