Optimized synthesis of lipase‐catalyzed biodiesel by Novozym 435

Abstract

The ability of immobilized lipase from Candida antarctica (Novozym 435) to catalyze the alcoholysis of canola oil and methanol was investigated. Response surface methodology (RSM) and five-level-five-factor central composite rotatable design (CCRD) were employed to evaluate the effects of synthesis parameters, such as reaction time, temperature, enzyme concentration, substrate molar ratio of methanol to canola oil, and added water content on percentage weight conversion of canola oil methyl ester by alcoholysis. Reaction temperature and enzyme concentration were the most important variables. High temperature and superabundant methanol inhibited the ability of Novozym 435 to catalyze the synthesis of biodiesel. Based on the analysis of ridge max, the optimum synthesis conditions were as follows: reaction time 12.4 h, temperature 38.0• C, enzyme concentration 42.3%, substrate molar ratio 3.5:1, and added water 7.2%. The predicted value was 99.4% weight conversion, and the actual experimental value was 97.9% weight conversion.

Combi-protein coated microcrystals of lipases for production of biodiesel from oil from spent coffee grounds

“…This screen was based upon 24 h reaction time to get an estimate of the highest conversion obtainable (irrespective of the rate of the reaction) with a particular enzyme preparation. Novozym 435, which is known to give fairly high rates for the enzymatic transesterification reaction for biodiesel conversion with various oils [25,57,58], again gave the highest conversion which was 88% in 24 h and the next best result was obtained with RMIM, which gave a conversion of 70% in 24 h. RMIM is a 1,3-specific enzyme and thus its theoretical yield is expected to be 66%. Yield higher than the theoretical yield may be due to a small amount of acyl-migration.…”

Cold active lipases – an update

“…The use of cold-adapted lipase in detergents would be of great advantage since they specifically Pseudomonas fluorescens P38 Tan et al (1996) Environmental applications Bioremediation and bioaugumentation Acinetobacter sp. 6 Suzuki et al (2001) Leather industry Degreasing using alkaline and acid lipases Lipases of commercial sources Afsar and Cetinkaya (2008) Other applications Biodiesel synthesis from degummed soybean oil CAL-B from Candida antarctica Watanabe et al (2002) Lipase-catalysed biodiesel CAL-B from Candida antarctica Chang et al (2005) allow washing under cold conditions which in turn decrease energy utilization and wear and tear of cloth fibres (Feller & Gerday 2003). The other advantages include reduced environmental load of detergent products, reduced use of chemicals in detergents, biodegradable, no negative impact on disposal of domestic waste and no risk for aquatic organisms.…”

An Organic Soluble Lipase for Water-Free Synthesis of Biodiesel

“…After 24 h of reaction, the conversion of soybean oil reached 71%. Accounting the amount of enzyme applied (1 mg/ml), the initial reaction rate was calculated as 33 g-product/g-enzyme h, which is much higher than those reported for Novozym 435 [19] and Lipozyme TL IM (0.25 g-product /g-enzyme h) [20].…”