Berries such as strawberries, blueberries and raspberries have expanded into non-traditional growing areas worldwide. In recent years, the berry production system has changed to cultivation in substrate and pots and this alternative system has shown the potential to produce high yields of high-quality berries. The quality of the substrate and the parameters of the pot are known to influence plant growth and fruit production. Many authors have evaluated the composition of the growing media and the size of the container with the aim of optimizing the appropriate growing conditions that affect the plant growth and berry yield. The physical characteristics of the substrate play a special role in berry cultivation; then, a good substrate should be of high quality, in order to provide the plant root system with the adequate environment to develop. The management of water irrigation and nutrient contents during berry production in substrates has a great importance on the yield and quality of fruits. Thus, suitable irrigation systems and a fertilizer solution containing all the essential nutrients are essential for yield and high-quality berries. In this perspective, the objective of this chapter is to present the equipment and practices used for the cultivation of berries in substrate.