Optimizing a rotor‐stator filter matrix for high‐gradient magnetic separation of functionalized magnetic particles

Shaikh, Yonas S.; Seibert, Christian; Schumann, C.; Ferner, Marvin J.; Raddatz, Heike; Kampeis, Percy

doi:10.1002/elsc.201500115

Cited by 6 publications

(5 citation statements)

References 20 publications

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“…The particle concentration in the filtrate was measured at the outlet of the HGMS filter with an UV/VIS spectrometer Ultrospec 2100pro from the company GE Healthcare equipped with a flow‐through cell at the wavelength of 600 nm. The measurement is explained in detail in .…”

Section: Methodsmentioning

confidence: 99%

Development of a novel disposable filter bag for separation of biomolecules with functionalized magnetic particles

Shaikh¹,

Kampeis²

2017

Engineering in Life Sciences

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The integration of disposable magnetic filters in combination with functionalized magnetic particles represents a fast and cost-effective alternative for enzyme purification in comparison to solid/liquid separation by means of centrifugation followed by chromatographic purification. The main advantage of the particle-based process is the solid/solid/liquid separation in one step combined with disposable equipment. Furthermore this combination provides the possibility to also process biocatalytic reactions in cell-containing media into disposable equipment with preimmobilized enzymes onto the magnetic particles. The focus of the presented study is on the design and performance of a disposable filtration unit consisting of a plastic bag with an inlet and outlet and a stainless steel filter matrix. During magnetic separation, the magnetic particles selectively retard at the filter matrix due to the magnetic force, which counteracts the drag force. It was found that the length of a lengthwise aligned filter matrix should be longer than the magnetic pole surfaces in fluid flow direction. Hereby, a filtration capacity of 5.6 g magnetic particles was measured with a loss of below 0.5%. Introducing a two-phase flow optimizes the cleaning of the bag after a magnetic filtration. The procedure offered a high cleaning efficiency. Herewith, the cleaned filter unit could be discarded with minimum losses of product and magnet particles.

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Section: Methodsmentioning

confidence: 99%

Development of a novel disposable filter bag for separation of biomolecules with functionalized magnetic particles

Shaikh¹,

Kampeis²

2017

Engineering in Life Sciences

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“…At the outlet of the magnetic filter the particle concentration in the filtrate was measured with an UV/VIS spectrometer Ultrospec 2100 pro from the company GE Healthcare which was equipped with a flow-through cell. The measurement is explained in detail in Shaikh et al [23]. For the quantification of the particle size distribution, samples were collected and measured with the particle analyzer Analysette 22 MicroTec from the company Fritsch GmbH.…”

Section: Methodsmentioning

confidence: 99%

Study on Optimizing High-Gradient Magnetic Separation—Part 2: Experimental Evaluation of the Performance of a New Designed Magnetic Filter

Shaikh¹,

Seibert²,

Kampeis³

2016

WJCMP

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The introduction of functionalized magnetizable particles and high-gradient magnetic separation represents a time and money saving alternative to conventional purification and separation unit operations in the biotechnical sector. This technique has some advantages especially for the recycling of immobilized enzymes. A new magnetic filter with sight glasses was constructed and produced to study the performance of high-gradient magnetic separation at varied parameters. By optical analysis the buildup of a clogging was identified as the major parameter which affected the separation performance. For the cleaning procedure, a two-phase flow of water with highly dispersed air bubbles was tested which led to a nearly complete cleaning of the filter chamber.

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“…Hereby ρ = density, u = linear velocity, p = pressure, ν = dynamic viscosity, F = Force, U 0 = average linear inlet velocity, I = unit vector and T is used as an indicator for matrix transposition. For the turbulent simulation of the fluid flow, the equations of the k-ε-model described in Shaikh et al [33] were used.…”

Section: Methodsmentioning

confidence: 99%

“…The particle concentration in the filtrate was measured at the outlet of the magnetic filter with an UV/VIS spectrometer Ultrospec 2100 pro from the company GE Healthcare equipped with a flow through cell. The measurement is explained in detail in Shaikh et al [33].…”

Section: Methodsmentioning

confidence: 99%

“…After injection the pump was started with a volume flow of 400 mL•min −1 . To ensure a complete particle run-through with an expected mean residence time of 20 s, the pumping was applied for 240 s. At the outlet of the filter chamber the concentration of particles was measured according to Shaikh et al [33] and used for the ratio calculation similar to the simulation. Figure 8 shows the particle retention results of the experiments (Figure 8(a)) and the results of the simulations (Figure 8(b)) as bar charts.…”

Section: Analysis and Optimization Of The Inlet Fluid Flowmentioning

confidence: 99%

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Study on Optimizing High-Gradient Magnetic Separation—Part 1: Improvement of Magnetic Particle Retention Based on CFD Simulations

Shaikh¹,

Seibert²,

Kampeis³

2016

WJCMP

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The introduction of functionalized magnetizable particles for the purification of enzymes or for the multi-use of pre-immobilized biocatalysts offers a great potential for time and cost savings in biotechnological process design. The selective separation of the magnetizable particles is performed for example by a high-gradient magnetic separator. In this study FEM and CFD simulations of the magnetic field and the fluid flow field within a filter chamber of a magnetic separator were carried out, to find an optimal separator design. The motion of virtual magnetizable particles was calculated with a one-way coupled Lagrangian approach in order to test many geometric and parametric variations in reduced time. It was found that a flow homogenisator smoothed the fluid flow, so that the linear velocity became nearly equal over the cross section in the direction of flow. Furthermore the retention of magnetizable particles increases with a high total edge length within the filter matrix.

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Optimizing a rotor‐stator filter matrix for high‐gradient magnetic separation of functionalized magnetic particles

Cited by 6 publications

References 20 publications

Development of a novel disposable filter bag for separation of biomolecules with functionalized magnetic particles

Development of a novel disposable filter bag for separation of biomolecules with functionalized magnetic particles

Study on Optimizing High-Gradient Magnetic Separation—Part 2: Experimental Evaluation of the Performance of a New Designed Magnetic Filter

Study on Optimizing High-Gradient Magnetic Separation—Part 1: Improvement of Magnetic Particle Retention Based on CFD Simulations

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