Optimizing Chitin Depolymerization by Lysozyme to Long-Chain Oligosaccharides

Abstract

Chitin oligosaccharides (COs) hold high promise as organic fertilizers in the ongoing agro-ecological transition. Short- and long-chain COs can contribute to the establishment of symbiotic associations between plants and microorganisms, facilitating the uptake of soil nutrients by host plants. Long-chain COs trigger plant innate immunity. A fine investigation of these different signaling pathways requires improving the access to high-purity COs. Here, we used the response surface methodology to optimize the production of COs by enzymatic hydrolysis of water-soluble chitin (WSC) with hen egg-white lysozyme. The influence of WSC concentration, its acetylation degree, and the reaction time course were modelled using a Box–Behnken design. Under optimized conditions, water-soluble COs up to the nonasaccharide were formed in 51% yield and purified to homogeneity. This straightforward approach opens new avenues to determine the complex roles of COs in plants.

A new group of LysM-RLKs involved in symbiotic signal perception and arbuscular mycorrhiza establishment

“…LCO-V(C18:1,NMe,S) were purified from the rhizobial strain Rhizobium tropici . CO4, CO5, CO7 and CO8 were prepared as described in 40 . Labelling of LCO-V(C18:1,NMe) was performed as described in 41 .…”

The physico-chemical properties and sorption potentials of snail shell particulates, chitin, chitosan, and oxalic acid modified chitosan from <i>achatina fulica</i> shell

“…Demineralization was achieved by immersing the deproteinized snail shell in a 5.00% (v/v) HCl solution to remove minerals, primarily calcium carbonate (Masselin et al, 2021). The mass-to-solution ratio was maintained at 1:6 (w/v), and the mixture was agitated for 30 minutes at 150 rpm at room temperature (25 o C).…”

Quality and staling characteristics of white bread fortified with lysozyme-hydrolyzed mealworm powder (Tenebrio molitor L.)

“…Lysozymes have been used to produce low-molecular-weight chitins (LMWCs) ( Lin et al, 2009 ), and lysozyme-hydrolyzed LMWCs exhibit beneficial physiological activities, such as antibacterial, anti-hypertensive, and immune-enhancing properties ( Jeon et al, 2000 ; Kurita, 2001 ). Furthermore, the lysozyme is an industrial enzyme available commercially in large quantities at low costs and have a wide range of reaction conditions ( Masselin et al, 2021 ). Therefore, using lysozymes as chitinolytic enzymes provides several benefits for industrial application.…”