Optimizing Colorimetric Assay Based on V2O5 Nanozymes for Sensitive Detection of H2O2 and Glucose

Abstract

Nanozyme-based chemical sensing is a rapidly emerging field of research. Herein, a simple colorimetric assay for the detection of hydrogen peroxide and glucose based on the peroxidase-like activity of V2O5 nanozymes has been established. In this assay, the effects of pH, substrate, nanozyme concentrations and buffer solution have been investigated. It was found that compared with 3,3′,5,5′-tetramethylbenzidine (TMB), the enzyme substrate o-phenylenediamine (OPD) seriously interfered with the H2O2 detection. Under the optimal reaction conditions, the resulting sensor displayed a good response to H2O2 with a linear range of 1 to 500 μM, and a detection limit of 1 μM at a signal-to-noise ratio of 3. A linear correlation was established between absorbance intensity and concentration of glucose from 10 to 2000 μM, with a detection limit of 10 μM. The current work presents a simple, cheap, more convenient, sensitive, and easy handling colorimetric assay.

Peroxidase‐Like Activity of Smart Nanomaterials and Their Advanced Application in Colorimetric Glucose Biosensors

“…Other metal oxide nanomaterials such as MnO 2 nanowires, V 2 O 5 nanowires, NiO NPs and NiC O2 O 4 mesoporous spheres are confirmed to possess intrinsic peroxidase‐like activity and used to fabricate colorimetric glucose biosensor as well (see Table 3 ). Those peroxidase‐like of metal oxides‐based nanomaterials are show a good selectivity and sensitivity for the detection of glucose.…”

Thin-film SnO<sub>2</sub> and ZnO detectors of hydrogen peroxide vapors

“…The permissible limit of exposure of 1,0 ppm has been established by the Occupational Safety and Health Administration (OSHA, USA; Corveleyn et al, 1997;Oberländer et al, 2014). It is immediately dangerous for life and health when its concentration reaches 75 ppm (Sun et al, 2016). Therefore, it was necessary to investigate the gas sensing characteristics of prepared sensors made from doped metal oxide films at low concentrations of HPV.…”

“…Several techniques such as chemiluminescence (Hsu et al, 2015), spectrophotometry (Rahim et al, 2011), fluorimetric and colorimetric detection (Sun et al, 2016), liquid chromatography, and electroanalytical and optical interferometry (Ensafi et al, 2016) have been developed for a reliable and sensitive determination of H 2 O 2 . These techniques are complex, expensive and time-consuming.…”

“…Note that the process of chemical decontamination can be carried out in two different ways: the first one is a wet approach using water or any other solutions of H 2 O 2 (certain concentration) and the second one is a dry method using H 2 O 2 in the vapor phase (Kačer et al, 2012). Therefore, the development and manufacturing of stable and reproducible sensors sensitive to hydrogen peroxide vapors (HPV) are urgently required (Taizo et al, 1998;Oberländer et al, 2014). The checking of the HPV phase is also crucially significant in connection with counterterrorism efforts.…”

Nanozyme applications in biology and medicine: an overview

“…Fe 3 O 4 MNPs function is on the base of Carboxyl-modified letter oxide (GOCOOH) to possess intrinsic peroxidase-like activity and catalyze the reaction of peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H 2 O 2 to produce a blue colour reaction [63]. Highly sensitive and selective colorimetric method for glucose detection has developed in a buffer solution or diluted blood and fruit juice samples [64,65]. Glucose detection is a promising method in clinical and food analysis [66].…”