Optimizing conditions to construct artificial cells using commercial in vitro transcription-translation system (PUREfrex2.0)

Sato, Takahiro; Matsuda, Shuhei; Aoki, Wataru

doi:10.1016/j.jbiosc.2023.07.004

Journal of Bioscience and Bioengineering

2023

DOI: 10.1016/j.jbiosc.2023.07.004

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Optimizing conditions to construct artificial cells using commercial in vitro transcription-translation system (PUREfrex2.0)

Takahiro Sato

Shuhei Matsuda²,

Wataru Aoki

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2024

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An Experimental Study on the Addition of Bacteria to Residual Anticancer Drugs: Evaluation of the Effect on Bacterial Growth

Kikuchi,

Miyauchi,

Yamaguchi

et al. 2024

Jpn J Infect Dis

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Using anticancer drugs as an example, we examined the possibility of reusing residual drugs. The use of residual drugs is not widespread because of concerns regarding bacterial contamination. We mixed anticancer drugs with bacteria and investigated their effects on bacterial growth. The anticancer drugs carboplatin, paclitaxel, etoposide, irinotecan, methotrexate, and 5-fluorouracil (5-FU) were mixed with Staphylococcus aureus, Enterococcus faecalis, Serratia marcescens, and Escherichia coli. After a certain period, the number of bacteria was counted. Irinotecan showed no antibacterial activity, whereas 5-FU showed high antibacterial activity against the bacteria tested. The 5-FU also showed a minimum inhibitory concentration value in the range of 8-80 μg/mL, depending on the bacterial species. The 5-FU dose-dependently inhibited S. aureus growth at more than 0.8 µg/mL. Since protein synthesis systems are reportedly antibiotic targets, we used a cell-free protein synthesis system to confirm the mechanism of the anticancer agent's antibacterial activity. The 5-FU and methotrexate had direct inhibitory effects on protein synthesis. It is suggested that even if the residual drugs are contaminated with bacteria, there will be no microbial growth or microbes will be killed by the drug. With careful monitoring, the 5-FU could potentially be used for antimicrobial purposes.

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An Experimental Study on the Addition of Bacteria to Residual Anticancer Drugs: Evaluation of the Effect on Bacterial Growth

Kikuchi,

Miyauchi,

Yamaguchi

et al. 2024

Jpn J Infect Dis

View full text Add to dashboard Cite

show abstract

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Optimizing conditions to construct artificial cells using commercial in vitro transcription-translation system (PUREfrex2.0)

Cited by 1 publication

References 43 publications

An Experimental Study on the Addition of Bacteria to Residual Anticancer Drugs: Evaluation of the Effect on Bacterial Growth

An Experimental Study on the Addition of Bacteria to Residual Anticancer Drugs: Evaluation of the Effect on Bacterial Growth

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