Optimizing Extraction Process and Characterization of Antioxidant Ingredients from Chlorella Sorokiniana

Lai, Phoency F.-H.; Sun, Tsai Chien

doi:10.15406/mojfpt.2017.05.00114

Cited by 4 publications

(4 citation statements)

References 15 publications

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“…The peaks identified 1025 cm −1 in C. sorokiniana correspond to the -CH 2 OH groups and to the C-OH stretching and bending of the C-OH moiety of carbohydrates indicate a rich glycogen superficial layer. Carbohydrate FTIR analysis in this study was in agreement with that found by Dore et al (2013), Guo et al (2018), De Castro et al (2018), Fernando et al (2017, Lai andSun (2017), Alvares et al (2021) and Balan et al (2019) TransmiƩance(%)…”

Section: Discussionsupporting

confidence: 92%

“…According to our data the heated acid hydrolysis extraction method of Chlorella sp. polysaccharide yields ranged from14 to 19% which is in agreement with that (18%) of 80–90 °C water extraction method from the Chlorella source (Lai and Sun 2017 ). Also, Sui et al ( 2012 ) study showed an extracted polysaccharide yield ranged from 13 to 19% from Chlorella sp.…”

Section: Discussionsupporting

confidence: 85%

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Characterization, antioxidant and anticoagulant properties of exopolysaccharide from marine microalgae

et al. 2022

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The sulfated exopolysaccharide extracted from marine microalgae attracted considerable attention from both the nutraceutical and pharmaceutical industries. In the present study biomass of five marine microalgae were screened to find strains with high capacity for the production of sulfated exopolysaccharides. The anticoagulant and antioxidant activities of extracted sulfated polysaccharides were evaluated using activated partial thromboplastin time (aPTT), prothrombin time (PT), DPPH and ABTS assays, respectively. The sulfated polysaccharides extracted from Picochlorum sp. showed a strong DPPH scavenging effect with 85% antioxidant activity. The sulfated polysaccharides of Chlorella sorokiniana, Chlorella sp. (L2) and Chlorella sp. (D1) scavenged more than 90% of the ABTS radicals. However, the sulfated polysaccharide extracted from Chlorella sorokiniana, and Chlorella sp. (N4) showed anticoagulant properties. The dual anticoagulant-antioxidant activities in Chlorella sorokiniana could be explained by the combination of various factors including sulfate content and their binding site, monosaccharide residue and glycoside bond which are involved in the polysaccharide’s bioactivity.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 85%

Characterization, antioxidant and anticoagulant properties of exopolysaccharide from marine microalgae

et al. 2022

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“…The C. sorokiniana produced carotenoids at 0.69% dry weight under extremophilic conditions (Matsukawa et al 2000). A previous study by Lai (2017) showed that the ethanolic extract of C. sorokiniana had an IC50 of 7.36 mg/mL.…”

Section: Introductionmentioning

confidence: 94%

“…Microalgae have been applied in various fields, including energy (Li et al 2011;Makareviciene et al 2011) and health as antioxidants (Li et al 2007). Microalgae that have been known to have antioxidant activity, including Botryococcus (Rao et al 2006), Dunaliella (Herrero et al 2006), and Haematococcus (Cerón et al 2007), Chlorella (Lai, 2017;Napitupulu 2019), and 32 selected microalgae (Goiris et al 2012). The antioxidant activity of these microalgae is related to the produced metabolites.…”

Section: Introductionmentioning

confidence: 99%

Short Communication: Antioxidant activity of ethanol extract of Chlorella sorokiniana cultured in tofu wastewater

MURSANDI¹,

SUSANTY²,

NURHAYATI³

et al. 2022

Nusantara Biosci

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Abstract. Mursandi H, Susanty D, Nurhayati L, Okasari AA. 2022. Short Communication: Antioxidant activity of ethanol extract of Chlorella sorokiniana cultured in tofu wastewater. Nusantara Bioscience 14: 155-159. Microalgae are microorganisms that grow quickly and produce secondary metabolites with antioxidant activity. Antioxidants of microalgae can be utilized in various aspects such as cosmetics, pharmaceuticals, supplements, and feed. Microalgae utilization will be more profitable if the microalgae can be cultured on waste media. This study aims to determine the concentration of a suitable medium for the growth of Chlorella sorokiniana Shihira & R.W.Krauss, total flavonoids, total phenolics, and the potential of ethanolic extract of C. sorokiniana as an antioxidant. This study cultured the microalgae C. sorokiniana on tofu liquid waste media at various concentrations (15, 20, 25, and 30%). The growth of C. sorokiniana on the media was observed using a spectrophotometer at 680 nm wavelength. C. sorokiniana biomass was collected on the 7th day. The biomass was extracted using ethanol as a solvent. Phytochemical analysis was performed using the standard method, Total phenolic content, total flavonoid content, and antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) were conducted to determine the IC50 value. The results showed that the best growth of C. sorokiniana was on TLW media at a concentration of 30%. The ethanolic extract of C. sorokiniana showed the presence of alkaloids, flavonoids, steroids, tannins, and saponins. The total phenolic content in the ethanolic extract of C. sorokiniana was 18.39 ± 0.29 mgGAE/g, and the total flavonoid content was 31.93 ± 5,60 mgQE/g. The IC50 of the ethanolic extract of C. sorokiniana was 288.95 mg/L, which shows this extract has a potent antioxidant.

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Algal biorefinery models with self-sustainable closed loop approach: Trends and prospective for blue-bioeconomy

Mohan

Hemalatha

Chakraborty

et al. 2020

Bioresource Technology

127

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Optimizing Extraction Process and Characterization of Antioxidant Ingredients from Chlorella Sorokiniana

Cited by 4 publications

References 15 publications

Characterization, antioxidant and anticoagulant properties of exopolysaccharide from marine microalgae

Characterization, antioxidant and anticoagulant properties of exopolysaccharide from marine microalgae

Short Communication: Antioxidant activity of ethanol extract of Chlorella sorokiniana cultured in tofu wastewater

Algal biorefinery models with self-sustainable closed loop approach: Trends and prospective for blue-bioeconomy

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