Optimizing factors affecting development and propagation of Bletilla striata in a temporary immersion bioreactor system

Zhang, Baoqian; Song, Lei; Bekele, Lemma Dadi; Shi, Jing‐Shan; Jia, Qiong; Zhang, Benhou; Jin, Leilei; Duns, Gregory J.; Chen, Jishuang

doi:10.1016/j.scienta.2018.01.007

Cited by 32 publications

(16 citation statements)

References 24 publications

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“…The critical factor in TIS is the immersion time since it governs nutrient uptake and expression of hyperhydricity. Hence, the optimization of shoot proliferation and reducing hyperhydricity, it is essential to find the balance between immersion frequencies and immersion duration (Steingroewer et al, 2013;Georgiev et al, 2014;Regueira et al, 2018;Zhang et al, 2018). C -schematic diagram of a temporary immersion RITA ® system, 1-three-way solenoid valve, 2 -culture chamber, 3 -medium compartment Bioreactor propagation become increasingly preferred due to additional advantages as precise control of temperature, light, dissolved oxygen and carbon dioxide, improved mass transfer of nutrients and waste metabolites, reduced risk of contamination, providing an easier for the handle and scalable technology (Takayama and Akita, 1994;Mamun et al, 2015).…”

Section: Bioreactors Application For Plant Propagationmentioning

confidence: 99%

Application of bioreactor technology in plant propagation and secondary metabolite production

Yancheva¹,

Georgieva²,

Badjakov³

et al. 2019

j. cent. eur. agric.

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Plant tissue cultures have been widely used in both fundamental and applied types of research on various biological species, and the scientific interest to transfer that technology in industrial scale has been rapidly growing. The use of in vitro technology for commercial propagation of different plant species and the production of bioactive compounds from them has become profitable industry worldwide. In the past decades, the progress in plant tissue culture technology was directed towards the introduction of the liquid medium for cultivation under submerged conditions in different bioreactor types, and automation of the entire process. Some applications of modified bioreactor systems and their importance for the advancement of plant biotechnology in the fields of agriculture, medicine, and pharmacy are discussed in this review.

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Section: Bioreactors Application For Plant Propagationmentioning

confidence: 99%

Application of bioreactor technology in plant propagation and secondary metabolite production

Yancheva¹,

Georgieva²,

Badjakov³

et al. 2019

j. cent. eur. agric.

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“…As an alternative for traditional in vitro culture, temporary immersion systems (TIS) using liquid culture in bioreactors have been used to produce plants, including several prototypes that could improve in vitro photomixotrophic conditions and therefore increase plant production (Arencibia et al, 2017), as well as produce plants that are more adaptive to acclimatization ex vitro. Bioreactor technology could represent a significant progress for the industrial development of clonal propagation in queen palm as compared with the traditional micropropagation (Zhang et al, 2018). Therefore, the objective of this study was to evaluate TIS bioreactors for in vitro germination of queen palm.…”

Section: Introductionmentioning

confidence: 99%

In vitro germination of Syagrus romanzoffiana (Cham.) Glassman

Neto

Machado

Chagas

et al. 2020

PCC&M

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Syagrus romanzoffiana (Cham.) Glassman belongs to the Arecaceae family and is native to South America, with large importance as an ornamental plant. Therefore, the objective of this study was to evaluate TIS bioreactors for in vitro germination of queen palm. Traditional (agar-based) in vitro and TIS bioreactor technologies were compared. We evaluated parameters for the optimization of the tetrazolium test for seed viability and a protocol to increase germinated seedling survival after in vitro propagation. Fruits of S. romanzoffiana were collected in October 2017 from 5 individual trees located at the Tropical Research and Education Center, University of Florida. A total of 40 embryos were established in each culture system. The culture medium was refreshed every 30 days. Four replicates of 7 embryos were subjected to the tetrazolium test under different concentrations. Embryos were transferred to either a temporary immersion system in SETIS bioreactors using liquid medium or to test-tubes containing an agarbased semi-solid medium. After 45 days under in vitro conditions, germinated embryos were carefully removed from both the agar and liquid media, thoroughly washed and transferred to ex vitro conditions using small plastic pots. We have concluded that the TTC test can be successfully used to determine seed viability in S. romanzoffiana regardless of the TTC concentration evaluated in our study (0.25 to 1%). The traditional semi-solid in vitro system showed to be more effective for in vitro queen palm zygotic embryo germination compared to the TIS system. Seedlings from both systems showed similar growth characteristics.

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“…In TIS, plant material can periodically come in contact with the liquid medium and the environment in the systems can be renewed; thus, an efficient nutrient uptake can be made by the plant tissue and disorders like asphyxia and hyperhydricity can be eliminated (Escalona et al, 1999;Etienne and Berthouly, 2002). Bioreactors based on TIS have been successfully used for the micropropagation of many plant species due to their advantages when compared with semisolid and continuous immersion culture (Zhang et al, 2018). These types of bioreactors can reduce consumables and labor costs (McAlister et al, 2005).…”

mentioning

confidence: 99%

“…These types of bioreactors can reduce consumables and labor costs (McAlister et al, 2005). They minimize energy requirements and are more manually flexible (Zhang et al, 2018). Periodical temporary contact between the liquid medium and the plant material generally increases proliferation rates and plant growth (Albarr an et al, 2005).…”

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confidence: 99%

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Micropropagation of Stevia rebaudiana Bertoni Using RITA® Bioreactor

Bayraktar¹

2019

horts

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Stevia rebaudiana Bertoni is used for medicinal and food purposes. The effectiveness of different culture systems in Stevia micropropagation was evaluated. Node explants were cultured in semisolid or RITA Ò bioreactor containing woody plant medium (WPM) without plant growth regulators (PGRs). The effect of three medium volumes (100, 200, and 300 mL) and four immersion frequencies (10 seconds every 1, 4, 6, or 8 hours) were assessed. The shoots and roots developed simultaneously in the RITA Ò bioreactor treatment containing 100 mL of WPM at 10 seconds/4 hours immersion frequency and the healthiest plantlets were obtained from this RITA Ò treatment using 5 mL of medium per explant. The root induction rate was 100% in nonhyperhydrated shoots obtained from both semisolid and RITA Ò bioreactor, and all of them were successfully acclimatized. Thus, an economical and viable protocol was developed by performing the micropropagation in one step, eliminating the use of agar and PGRs, and decreasing the amount of medium used per explant.

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Optimizing factors affecting development and propagation of Bletilla striata in a temporary immersion bioreactor system

Cited by 32 publications

References 24 publications

Application of bioreactor technology in plant propagation and secondary metabolite production

Application of bioreactor technology in plant propagation and secondary metabolite production

In vitro germination of Syagrus romanzoffiana (Cham.) Glassman

Micropropagation of Stevia rebaudiana Bertoni Using RITA® Bioreactor

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