2024
DOI: 10.1007/s42729-024-01866-y
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Optimizing pH for Soil Enzyme Assays Reveals Important Biochemical Functions in Low pH Soil

Tandra D. Fraser,
Sarah Duddigan,
Anita Diaz
et al.

Abstract: Soil enzyme assays are often used as indicators of potential biological functions. The objective of this study was to understand enzyme activity across a range of soil pH. Soils (0–15 cm) were collected from a heathland restoration project (established 1999) on the Isle of Purbeck, UK with treatments of elemental sulphur or ferrous sulphate compared to a control, acid grassland and heathland. Enzyme assays were conducted using fluorescent substrates for β-1,4-glucosidase, β-N-acetylglucosaminidase (NAG) and ph… Show more

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