Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass

Gerde, José A.; Wang, Tong; Yao, Linxing; Jung, Stéphanie; Johnson, Lawrence A.; Lamsal, Buddhi P.

doi:10.1016/j.algal.2013.02.001

Cited by 131 publications

(102 citation statements)

References 29 publications

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“…These data demonstrate the effectiveness of the treatment. The results presented herein showed higher protein recoveries than those previously reported by Gerde et al (2013), who optimized the isolation of proteins from Nannochloropsis biomass obtaining protein recoveries close to 30 g protein per 100 g microalga and with protein concentrations close to 57%. …”

Section: Discussion Protein Recovery: Optimization Of the Solubilizatcontrasting

confidence: 52%

“…In this context, more conservative methods like sonication or high pressure cell disruption have been studied, reporting high protein solubilization (from 52.3 to 73.0%) of the total initial proteins in the microalga using pH 11 in Nannochloropsis sp. (Gerde et al, 2013), pH 12 in Chlorella vulgaris (Ursu et al, 2014) and pH 5.7 in Haematococcus pluvialis (Ba et al, 2016). These studies evidence the key role of the pretreatment method and the pH used to solubilize proteins despite of the microalga used.…”

Section: Introductionmentioning

confidence: 62%

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Recovery of Protein Concentrates From Microalgal Biomass Grown in Manure for Fish Feed and Valorization of the By-Products Through Anaerobic Digestion

Hernández

Molinuevo-Salces

Riaño

et al. 2018

Front. Sustain. Food Syst.

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Section: Discussion Protein Recovery: Optimization Of the Solubilizatcontrasting

confidence: 52%

Section: Introductionmentioning

confidence: 62%

Recovery of Protein Concentrates From Microalgal Biomass Grown in Manure for Fish Feed and Valorization of the By-Products Through Anaerobic Digestion

Hernández

Molinuevo-Salces

Riaño

et al. 2018

Front. Sustain. Food Syst.

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“…Thus, increasing the extraction efficiency largely depends on varying the physicochemical conditions like temperature, salinity, and pH of the aqueous solutions to increase the protein solubility. Gerde et al (2013) investigated the possibility of extracting protein from defatted and non-defatted Nannochloropsis biomass. They found that maximum amounts of protein are dissolved at 60°C and pH 11 and that it can be best recovered when the pH is decreased to 3.2.…”

Section: Extraction Of Proteins From Nannochloropsismentioning

confidence: 99%

A biorefinery for Nannochloropsis: Induction, harvesting, and extraction of EPA-rich oil and high-value protein

Chua

Schenk

2017

Bioresource Technology

131

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Please cite this article as: Chua, E.T., Schenk, P.M., A biorefinery for Nannochloropsis: induction, harvesting, and extraction of EPA-rich oil and high-value protein, Bioresource Technology (2017), doi: http://dx.doi.org/10. 1016/ j.biortech.2017.05.124 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractMicroalgae have been studied as biofactories for almost four decades. Yet, even until today, many aspects of microalgae farming and processing are still considered exploratory because of the uniqueness of each microalgal species. Thus, it is important to develop the entire process of microalgae farming: from culturing to harvesting, and down to extracting the desired high-value products. Based on its rapid growth and high oil productivities, Nannochloropsis sp. is of particular interest to many industries for the production of highvalue oil containing omega-3 fatty acids, specifically eicosapentaenoic acid (EPA), but also several other products. This review compares the various techniques for induction, harvesting, and extraction of EPA-rich oil and high-value protein explored by academia and industry to develop a multi-product Nannochloropsis biorefinery. Knowledge gaps and opportunities are discussed for culturing and inducing fatty acid biosynthesis, biomass harvesting, and extracting EPA-rich oil and high-value protein from the biomass of Nannochloropsis sp.

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“…High-pressure homogenization and ultrasonication were reported to enhance microalgal protein solubilization, the former being the most effective method (Gerde et al, 2013;Safi et al, 2014). Autoclaving was reported as an effective pretreatment to improve lipid extraction from microalgae (Prabakaran and Ravindran, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, solubilization under alkaline conditions followed by precipitation at isoelectric pH is a useful strategy for obtaining crude protein isolates. Several authors reported protein extraction from green algae and cyanobacteria using this method (Choi and Markakis, 1981;Chronakis et al, 2000;Gerde et al, 2013;Safi et al, 2014;Ursu et al, 2014). Other parameters that could impact protein solubility include extraction (solubilization or precipitation) time, solvent/biomass ratio (biomass concentration), and temperature (Abas Wani et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Optimization of Protein Extraction from Spirulina platensis to Generate a Potential Co-Product and a Biofuel Feedstock with Reduced Nitrogen Content

et al. 2015

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The current work reports protein extraction from Spirulina platensis cyanobacterial biomass in order to simultaneously generate a potential co-product and a biofuel feedstock with reduced nitrogen content. S. platensis cells were subjected to cell disruption by high-pressure homogenization and subsequent protein isolation by solubilization at alkaline pH followed by precipitation at acidic pH. Response surface methodology was used to optimize the process parameters -pH, extraction (solubilization/precipitation) time and biomass concentration for obtaining maximum protein yield. The optimized process conditions were found to be pH 11.38, solubilization time of 35 min and biomass concentration of 3.6% (w/w) solids for the solubilization step, and pH 4.01 and precipitation time of 60 min for the precipitation step. At the optimized conditions, a high protein yield of 60.7% (w/w) was obtained. The protein isolate (co-product) had a higher protein content [80.6% (w/w)], lower ash [1.9% (w/w)] and mineral content and was enriched in essential amino acids, the nutritious γ-linolenic acid and other high-value unsaturated fatty acids compared to the original biomass. The residual biomass obtained after protein extraction had lower nitrogen content and higher total non-protein content than the original biomass. The loss of about 50% of the total lipids from this fraction did not impact its composition significantly owing to the low lipid content of S. platensis (8.03%).

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Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass

Cited by 131 publications

References 29 publications

Recovery of Protein Concentrates From Microalgal Biomass Grown in Manure for Fish Feed and Valorization of the By-Products Through Anaerobic Digestion

Recovery of Protein Concentrates From Microalgal Biomass Grown in Manure for Fish Feed and Valorization of the By-Products Through Anaerobic Digestion

A biorefinery for Nannochloropsis: Induction, harvesting, and extraction of EPA-rich oil and high-value protein

Optimization of Protein Extraction from Spirulina platensis to Generate a Potential Co-Product and a Biofuel Feedstock with Reduced Nitrogen Content

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