Optimizing Resolution in Constant-Energy Synchronous Spectrofluorimetry

Eiroa, A. Andrade; Huckins, S.R.; Blanco, E. Vázquez; López-Mahı́a, Purificación; Muniategui-Lorenzo, Soledad; Rodriquez, Dario

doi:10.1366/0003702001948466

Cited by 9 publications

(4 citation statements)

References 19 publications

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“…It is clear from the above discussion that both CWSS and CESS techniques could be used for estimation of the cited drugs with high sensitivity down to nanogram levels. In comparison between the two developed methods, the CESS offered an improvement in sensitivity and selectivity compared to the more conventional CWSS technique, which was in agreement with the reported literature [58]. This can be attributed to the reduction of solvent Raman scatter and Rayleigh scatter interferences with the fluorescence spectra of the analytes [59].…”

Section: Comparison Between the Two Developed Methodssupporting

confidence: 87%

Two different synchronous spectrofluorimetric approaches for simultaneous determination of febuxostat and ibuprofen

et al. 2021

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Two green, simple and sensitive synchronous spectrofluorimetric methods were developed for the first time for the simultaneous estimation of febuxostat (FEB) and ibuprofen (IBU). Method I is constant-wavelength synchronous spectrofluorimetry where FEB and IBU were recorded at 329 and 258 nm, respectively, using Δ λ of 40 nm. Method II is constant-energy synchronous spectrofluorimetry using a wavenumber interval of −4000 cm −1 . All measurements were carried out in a borate buffer of pH 7 and distilled water for dilution which increased the methods' greenness. The two methods were rectilinear over concentration ranges of 30.0–700.0 ng ml −1 and 0.5–9.0 µg ml −1 in the first method and 20.0–500.0 ng ml −1 and 0.1–8.0 µg ml −1 in the second method for FEB and IBU, respectively. High sensitivity was attained for the two drugs with limits of quantitations (LODs) down to 0.41 and 5.51 ng ml −1 in the first method and 0.25 and 3.32 ng ml −1 in the second method for FEB and IBU, respectively. Recovery percentages were in the range of 97.3–101.9% after extraction from spiked human plasma samples, demonstrating high bioanalytical applicability. The two methods were further applied to tablet dosage forms with good recovery results. The methods' greenness was assessed according to the analytical Eco-Scale and Green Analytical Procedure Index guidelines.

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Section: Comparison Between the Two Developed Methodssupporting

confidence: 87%

Two different synchronous spectrofluorimetric approaches for simultaneous determination of febuxostat and ibuprofen

et al. 2021

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“…For multifluorophoric samples at higher concentrations, despite the presence of factors such as light attenuation (LA), self-absorption, energy transfer (ET) and collisional quenching (CQ), it has been observed earlier that the use of right-angle sample geometry is capable of providing better analytical utility, as compared to front-face or 45°-angle sample geometry [6,7]. Synchronous fluorescence scan (SFS) gives a narrower and simpler spectrum and is of extensive use for multi-fluorophoric analysis [6,7,8,9,10,11,12,13,14,15,16,17,18,19,20]. In this method, both excitation and emission monochromators are scanned simultaneously keeping a fixed wavelength interval (∆λ) between them (constant ∆λ mode).…”

Section: Introductionmentioning

confidence: 99%

Total synchronous fluorescence scan spectra of petroleum products

2002

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Extending the two-dimensional synchronous fluorescence scan to a three-dimensional total synchronous fluorescence scan (TSFS) spectral measurement gives the total synchronous fluorescence characteristics of a multifluorophoric sample at various possible wavelength intervals (Deltalambda), which could help to characterize multifluorophoric systems better. TSFS spectra of petroleum products such as diesel, kerosene, petrol, engine oil etc., available in the Indian market, are reported. Fluorescence in these samples is due to the presence of polycyclic aromatic hydrocarbons (PAHs) of various ring sizes. The TSFS contour plot profiles of the neat samples measured at right-angle geometry is a result of various energy-degrading photophysical processes such as inner filter effect, light attenuation, resonance energy transfer, collisional quenching etc. TSFS plots make it easy to obtain the optimized Deltalambda of an unknown sample of analytical interest. TSFS and the excitation-emission matrix (EEM) techniques are similar, but the contour profiles generated are different. The response of the TSFS contour profiles to dilution is different from that in the EEM contour profiles. Thus, TSFS can provide an alternative way of presenting the fluorescence response of concentrated multifluorophoric samples.

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“…This complication can be overcome by using special techniques such as synchronous spectrometry. Constant-energy synchronous luminescence spectrometry (CESLS) [9,10] , one important embranchment, has been successfully applied to BaP identification in PAHs mixture [11][12][13][14] .…”

Section: Introductionmentioning

confidence: 99%

Simple synchronous fluorimetric method for determination of benzo[a]pyrene in airborne particulates without a pre-separation procedure

Zhang

2007

Wuhan Univ. J. of Nat. Sci.

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A sensitive analytical method, constant-energy synchronous fluorimetry, has been used to determine benzo[a]pyrene (BaP) selectively in airborne particulates (AP). A constant-energy difference of 1 400 cm -1 was selected for obtaining single spectral peak. The method could easily identify BaP in an 18-component polycylic aromatic hydrocarbons(PAHs) mixtures. Calibration plots in methanol solution and in sodium dodecylsulfate (SDS) micellar media show a good linearity (R>0.999) in the BaP concentration range generally found in the environment. The method gives a detection limit of 0.40 nmol/L in SDS micellar medium and 1.34 nmol/L in methanol solution.

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Optimizing Resolution in Constant-Energy Synchronous Spectrofluorimetry

Cited by 9 publications

References 19 publications

Two different synchronous spectrofluorimetric approaches for simultaneous determination of febuxostat and ibuprofen

Two different synchronous spectrofluorimetric approaches for simultaneous determination of febuxostat and ibuprofen

Total synchronous fluorescence scan spectra of petroleum products

Simple synchronous fluorimetric method for determination of benzo[a]pyrene in airborne particulates without a pre-separation procedure

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