Optimizing Sampling and Extraction Methods for Plant-Parasitic and Entomopathogenic Nematodes

Abd-Elgawad, Mahfouz M. M.

doi:10.3390/plants10040629

Cited by 30 publications

(23 citation statements)

References 59 publications

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“…Globally, the prevalence of EPNs in soil samples was reported to range from 0.7 to 50% ( Benseddiq et al, 2020 ; Hominick, 2002 ) though specific sampling methods with repeated extraction technique may exceed this range ( Abd-Elgawad, 2020 ). Merits and demerits of nematode-sampling and extraction methods were recently reviewed ( Abd-Elgawad, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of indigenous entomopathogenic nematodes in Southwest China as potential biocontrol agents against Spodoptera litura (Lepidoptera: Noctuidae)

Sun

Zhang

Song³

et al. 2021

Journal of Nematology

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Spodoptera litura is a notorious leaf feeding insect pest in the Asia-Pacific region and leads to a significant economic loss in vegetable and field crop production. Entomopathogenic nematodes (EPNs), lethal parasites of insects, are used as biocontrol agents. Yunnan Province in China is a well-known region due to its rich biodiversity. In the present study, a survey of EPNs using the Galleria-baiting technique was conducted in 2017 and 2018 throughout the entire Yunnan province. In total, 789 soil samples were collected from 232 sites, of which 75 samples were positive for EPNs. Phylogenetic analyses of ITS, D2D3 expansion region of the 28S rRNA gene, as well as mitochondrial cytochrome c oxidase subunit I (COI), were performed to identify isolated nematode species and evaluate their genetic diversity. In total, 13, 3, and 58 identified populations belong to Steinernema, Heterorhabditis, and Oscheius, respectively. The phylogenetic relationships of EPN species in the three genera were analyzed with the Neighbor-Joining method. The virulence of the trapped isolates in the genera of Steinernema, Heterorhabditis, and Oscheius against S. litura was evaluated. Ten new indigenous isolates from Steinernema and Heterorhabditis showed prominent virulence to S. litura within 48 hr which is equivalent to that of commercial EPNs populations. The present study provides background information on indigenous EPN resources for S. litura control in Asia-Pacific region.

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Section: Discussionmentioning

confidence: 99%

Evaluation of indigenous entomopathogenic nematodes in Southwest China as potential biocontrol agents against Spodoptera litura (Lepidoptera: Noctuidae)

Sun

Zhang

Song³

et al. 2021

Journal of Nematology

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“…Due to the above-mentioned huge numbers and many species of pests and pathogens of Egyptian citrus, the following part will focus on an important group of these pests, the plant-parasitic nematodes. Factually, PPNs and entomopathogenic nematodes (EPNs) are two important sets as harmful and beneficial organisms, respectively in crop production systems (Abd-Elgawad, 2021b). In this respect, the proportion of nematode injury to any plant species is also related to PPN species/race, densities, and existing conditions, abiotic and biotic factors.…”

Section: Plant-parasitic Nematodes Of Citrus In Egyptmentioning

confidence: 99%

Management of Pests and Pathogens Affecting Citrus Yield in Egypt with Special Emphasis on Nematodes

Hammam

Gawad

Ruan

et al. 2021

Egyptian Journal of Agronematology

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Although citriculture ranks high in Egypt as a superior orange producer and exporter country, citrus yield should be further optimized via management of pests and pathogens that adversely affect citrus orchards. We throw light on the various groups of these citrus-damaging organisms to alarm for the significance of adopting adequate programs for their control. As many plant-parasitic nematode (PPN) species in Egypt and abroad; all but one, the citrus nematode Tylenchulus semipenetrans, of which are of limited distribution, this species was addressed herein in details. The citrus nematode could significantly reduce lemon yield. Before nematicidal application, growers and stakeholders should adopt strict quarantine and seedling certification programs, consider the limiting factor principle, and use appropriate nematode sampling and extraction methods. Moreover, bionematicides should be earnestly tried among other PPN management options in order to avoid human health hazards and attain pollution-free environment. Yet, holistic management should include not only nematodes but also other pests and pathogens of citrus in Egypt. We highlighted general production practices that should adequately tackle relevant citrus problems.

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“…Although optimizing PPN sampling and extraction methods to avoid their misuse and achieve cost-effective and efficient IPM programs are recently emphasized [ 100 ], such advances for PPN control should biotechnologically keep up in parallel to these improvements. The current research on genome sequencing technologies, small interfering RNA techniques (RNAi), and targeted genome editing should be harnessed to better grasp plant–nematode interaction mechanisms, and molecular enhancing of PPN-plant resistance should be used to boost these programs [ 1 ].…”

Section: Other Methods Of Ppn Managementmentioning

confidence: 99%

Optimizing Safe Approaches to Manage Plant-Parasitic Nematodes

Abd-Elgawad

2021

Plants

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Plant-parasitic nematodes (PPNs) infect and cause substantial yield losses of many foods, feed, and fiber crops. Increasing concern over chemical nematicides has increased interest in safe alternative methods to minimize these losses. This review focuses on the use and potential of current methods such as biologicals, botanicals, non-host crops, and related rotations, as well as modern techniques against PPNs in sustainable agroecosystems. To evaluate their potential for control, this review offers overviews of their interactions with other biotic and abiotic factors from the standpoint of PPN management. The positive or negative roles of specific production practices are assessed in the context of integrated pest management. Examples are given to reinforce PPN control and increase crop yields via dual-purpose, sequential, and co-application of agricultural inputs. The involved PPN control mechanisms were reviewed with suggestions to optimize their gains. Using the biologicals would preferably be backed by agricultural conservation practices to face issues related to their reliability, inconsistency, and slow activity against PPNs. These practices may comprise offering supplementary resources, such as adequate organic matter, enhancing their habitat quality via specific soil amendments, and reducing or avoiding negative influences of pesticides. Soil microbiome and planted genotypes should be manipulated in specific nematode-suppressive soils to conserve native biologicals that serve to control PPNs. Culture-dependent techniques may be expanded to use promising microbial groups of the suppressive soils to recycle in their host populations. Other modern techniques for PPN control are discussed to maximize their efficient use.

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Optimizing Sampling and Extraction Methods for Plant-Parasitic and Entomopathogenic Nematodes

Cited by 30 publications

References 59 publications

Evaluation of indigenous entomopathogenic nematodes in Southwest China as potential biocontrol agents against Spodoptera litura (Lepidoptera: Noctuidae)

Evaluation of indigenous entomopathogenic nematodes in Southwest China as potential biocontrol agents against Spodoptera litura (Lepidoptera: Noctuidae)

Management of Pests and Pathogens Affecting Citrus Yield in Egypt with Special Emphasis on Nematodes

Optimizing Safe Approaches to Manage Plant-Parasitic Nematodes

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