Optimizing the biosynthesis of renewable polyhydroxyalkanoate copolymer containing 3-hydroxyvalerate by Massilia haematophila using statistical modeling

Abstract

Polyhydroxyalkanoate (PHA) is a microbial storage polymer that is naturally produced by certain bacteria. This is the first study on the ability of this particular species Massilia haematophila to synthesize a PHA copolymer containing 3-hydroxyvalerate (3HV) monomer. Using the statistical design on Massilia haematophila UMTKB-2, this study highlights the optimization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV), copolymer production for shaken-flask cultivation. Moreover, the mechanical and thermal features of the polymers were determined. The production of P(3HB-co-3HV) by Massilia haematophila UMTKB-2 using optimal conditions provided by response surface methodology (RSM) yielded 5.0 g/l of P(3HB-co-7 mol% 3HV), which was higher than the value obtained from unoptimized conditions such as 4.40 g/l of P(3HB-co-4mol% 3HV). This result showed a 14% increase in copolymer concentration and a twofold increase in 3HV composition. In this study, the P(3HB-co-3HV) synthesized was determined as a block copolymer and its thermal properties were better than P(3HB). Using RSM, the optimization conditions were successfully obtained for this bacterium, and this result is a starting platform for additional studies of a larger scaled PHA production from Massilia haematophila UMTKB-2 using bioreactors.

Polyhydroxyalkanoate (PHA) Biopolymer Synthesis by Marine Bacteria of the Malaysian Coral Triangle Region and Mining for PHA Synthase Genes

“…The marine sponge-associated bacterial diversity profiling revealed that eight out of the ten most dominant genera and four out of the ten most dominant families were previously published PHA producers ( Figure 7 ) [ 51 , 52 ]. However, not all species classified as PHA-producing genera are experimentally shown to produce PHA.…”

A Critical Review on the Economically Feasible and Sustainable Poly(3-Hydroxybutyrate-co-3-hydroxyvalerate) Production from Alkyl Alcohols

“…Unlike the PHA accumulation process of Cupriavidus sp. that ends within 48-72 h, 122 h was needed for optimum P(3HB-co-3HV) accumulation by M. haematophila UMTKB-2 [89] (Table 2).…”

Isolation, molecular identification, and genomic analysis of Mangrovibacter phragmitis strain ASIOC01 from activated sludge harboring the bioremediation prowess of glycerol and organic pollutants in high-salinity

“…The primary sources of MGB pure cultures were predominantly obtained from the regions of East Asia, South East Asia, and South Asia and extensively identified in various habitats ( Table 1 , Supplementary Tables S1A , S1B ) using 16S rRNA-based amplicon sequencing analysis. The distribution of MGBs includes high-salt sludges ( Li et al, 2019 , 2020 ; Yin et al, 2022 ), tannery effluent ( Lian et al, 2016a , b ; Sanjay et al, 2020 ), brackish water ( Kiun et al, 2016 ), mangrove rhizosphere sediment (BioProject no PRJNA682484), farm soil ( Zhang et al, 2015 ), shrimp aquaculture farm ( Joseph et al, 2014 ), storage tanks containing biodiesel ( White et al, 2011 ), water droplets from within the bitumen ( Himmelberg, 2019 ), geothermal lake (MYI strain CEMTC_1,393, GenBank accession no. OP143686.1), and pond (“Shoubuike”) ( Fujita et al, 2023 ).…”

“…Several experiments employed nutrient-rich media to facilitate the growth of MGBs, including bacto marine broth, LB broth supplemented with 15% (v/v) glycerol ( Zhang et al, 2015 ), LB agar ( Behera et al, 2017 ), brain heart infusion agar ( Sanjay et al, 2020 ), and a medium consisting of meat extract, yeast extract, and peptone ( Kiun et al, 2016 ). These nutrient-rich media are not recommended due to their inability to eradicate undesirable microbial flora effectively.…”