Many biological signals must be measured and interpreted accurately to titrate therapy properly during donor care. Although the technological aspects of intravascular pressure monitoring are usually delegated to bedside nursing colleagues, organ procurement coordinators should be familiar with those devices and methods. The equipment, supplies, and procedures used for arterial and central venous pressure monitoring are reviewed. Transducer leveling and zeroing plus maintenance of the hydraulic tubing system between the transducer and bedside monitor are especially important. These variables may greatly influence the accuracy of the displayed pressures and, therefore, must be considered during donor assessment as treatment is considered.