Optimizing the Extraction of Acid-soluble Collagen Inside the Eggshell Membrane

Kheirabadi, Elahe Kazemi; Razavi, Seyed Hadi; Khodaiyan, Faramarz; Golmakani, Mohammad‐Taghi

doi:10.3136/fstr.24.385

Cited by 9 publications

(4 citation statements)

References 36 publications

(37 reference statements)

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“…Eggshells are widely used as fertilizers, feed supplements, absorbent media in fillets, medical and pharmaceutical products. The utilization and use of the shell membrane is in interest of the medicine as a source of collagen type I, V & X, keratin and other substance that stimulate the synthesis of collagen and wound healing [12][13].…”

Section: Introductionmentioning

confidence: 99%

Examining coagulation conditions for the utilization of technical albumen. A preliminary study

Saraliev,

Balev,

Vlahova-Vangelova

et al. 2024

BIO Web Conf.

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Egg processing, a major source of high-value protein for the food industry, is on the rise. However, it generates significant waste. Utilizing egg by-products, especially technical albumen, offers ways to create value-added products. The aim of this study is to examined different coagulation conditions for the utilization of technical albumen. Four types of coagulation were examined. The first type required pH correction using an acid solution (30% citric, lactic, or acetic acid) until the pH ranged from 4.8 to 5.2, followed by thermal coagulation using steam. The second type involved mixing the technical albumen with various percentages of 96% ethanol, ranging from 1% to 10%, followed by coagulation using steam. The third type was a combination of the first two. The precipitates were collected in porous fibre and pressed for 15 minutes at 350 gr/cm2. Citric acid led to grainy, high-yield precipitates; lactic and acetic acid yielded more homogenous precipitates with increased dry matter loss. Only the 7.5% addition of ethanol resulted in relatively high precipitate yield. The combination of pH correction and ethanol addition didn’t enhance yield. Understanding the correlation between the composition of the technical albumen and applied coagulation condition necessitates further analysis.

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Section: Introductionmentioning

confidence: 99%

Examining coagulation conditions for the utilization of technical albumen. A preliminary study

Saraliev,

Balev,

Vlahova-Vangelova

et al. 2024

BIO Web Conf.

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“…12,19 The second option is chemical treatment by reagents such as EDTA, chlorine, and hydrochloric or acetic acid. [20][21][22][23] The third separation technique is related to physical processes such as oatation or using a centrifugal separator. There are also different approaches for the conversion process.…”

Section: Introductionmentioning

confidence: 99%

Scalable Production of Bio-Calcium Oxide via Thermal Decomposition of Solid - Hatchery Waste in a Laboratory-Scale Rotary Kiln

Chuakham,

Putkham,

Chaiyachet

et al. 2024

Preprint

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Chicken eggshell waste is an alternative renewable source for quicklime production. Eggshell waste has received significant attention from researchers due to it being a potential source of bio-CaO, which not only drives the circular economy concept but also supports sustainable development. However, experiments on the production of bio-CaO are normally conducted in a small lab-scale furnace. Furthermore, the eggshell raw material is collected from canteens or households, which is not suitable for economical or industrial production. Therefore, this study investigated the factors affecting the bio-CaO production from hatchery waste via both batch and continuous calcination process in a laboratory-scale rotary kiln for the first time. The eggshells were first separated from the solid hatchery waste. Then, the effect of preparation methods of raw eggshells on the properties of bio-CaO was investigated, e.g., eggshells with and without membrane separation, various particle sizes, and with an increase of the percent raw material filling in the kiln from 5–20%. Calcination of the samples was performed in a rotary kiln at 800°C with a 0.5 RPM rotating speed and a 5° inclination of the kiln. The effects of the calcination process in either an air or N2 atmosphere on the calcined product were also observed. Instrumental analysis shows that the production yield and purity of bio-CaO were in the range of 49–56 wt% and 97–98%, respectively. The results also indicated that the production yield of bio-CaO decreased to 17.7% with a decrease in the raw material particle size from 3.3 mm to 250 µm. Moreover, the production of bio-CaO with eggshells containing eggshell membrane decreases the purity of calcium oxide by about 0.7–1.0%. In addition, further increasing the filling volume of the kiln from 5–20% had only a slight effect on the purity and yield of the product. These results imply that it is not necessary to remove the eggshell membrane from the raw eggshells in order to produce industrial-grade CaO from the raw eggshell. These new findings can likely be used to develop an alternative process design to reduce the manufacturing cost of bio-CaO produced from hatchery waste. Furthermore, this present study reveals that the specifications of the obtained bio-CaO comply with both Thai industrial standards and international food additive standards.

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“…Likewise, the high surface area of the ESM lets it serve as an adsorbent of drugs, nanoparticles, or microparticles to improve tissue regeneration via controlled drug release [ 7 , 9 ]. Based on these characteristics, the ESM have been exploited as a potential biomaterial for dermal wound-healing applications [ 7 , 9 , 11 , 12 , 13 , 14 , 15 ]. One important process for the use of ESM as a biomaterial is its separation from the eggshell (ES).…”

Section: Introductionmentioning

confidence: 99%

A Sustainable, Green-Processed, Ag-Nanoparticle-Incorporated Eggshell-Derived Biomaterial for Wound-Healing Applications

Mensah,

Trotta,

Briggs

et al. 2023

JFB

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The eggshell membrane (ESM) is a natural biomaterial with unique physical and mechanical properties that make it a promising candidate for wound-healing applications. However, the ESM’s inherent properties can be enhanced through incorporation of silver nanoparticles (AgNPs), which have been shown to have antimicrobial properties. In this study, commercially produced AgNPs and green-processed AgNPs were incorporated into ESM and evaluated for their physical, biological, and antimicrobial properties for potential dermal application. The ESM was extracted using various techniques, and then treated with either commercially produced AgNPs (Sigma-Aldrich, Poole, UK) or green-synthesized AgNPs (Metalchemy, London, UK) to produce AgNPs-ESM samples. The physical characteristics of the samples were evaluated using scanning electron microscopy (SEM), Fourier Transform Infrared (FTIR) spectroscopy, and the biological properties were assessed through in vitro studies using human dermal fibroblasts (HDFs) and BJ cells. The SEM analysis of the AgNPs-ESM samples showed localization of AgNPs on the ESM surface, and that the ESM maintained its structural integrity following AgNP incorporation. The FTIR confirmed loading of AgNPs to ESM samples. The biological studies showed that the 5 μg/mL AgNPs-ESM samples were highly biocompatible with both HDFs and BJ cells, and had good viability and proliferation rates. Additionally, the AgNPs-ESM samples demonstrated pro-angiogenic properties in the CAM assay, indicating their potential for promoting new blood vessel growth. Assessment of the antimicrobial activity of the enhanced AgNPs/ESMs was validated using the International Standard ISO 16869:2008 methodology and exploited Cladosporium, which is one of the most commonly identified fungi in wounds, as the test microorganism (≥5 × 106 cells/mL). The AgNPs-ESM samples displayed promising antimicrobial efficacy as evidenced by the measured zone of inhibition. Notably, the green-synthesized AgNPs demonstrated greater zones of inhibition (~17 times larger) compared to commercially available AgNPs (Sigma-Aldrich). Although both types of AgNP exhibited long-term stability, the Metalchemy-modified samples demonstrated a slightly stronger inhibitory effect. Overall, the AgNPs-ESM samples developed in this study exhibited desirable physical, biological, and antimicrobial properties for potential dermal wound-dressing applications. The use of green-processed AgNPs in the fabrication of the AgNPs-ESM samples highlights the potential for sustainable and environmentally friendly wound-healing therapies. Further research is required to assess the long-term biocompatibility and effectiveness of these biomaterials in vivo.

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Optimizing the Extraction of Acid-soluble Collagen Inside the Eggshell Membrane

Cited by 9 publications

References 36 publications

Examining coagulation conditions for the utilization of technical albumen. A preliminary study

Examining coagulation conditions for the utilization of technical albumen. A preliminary study

Scalable Production of Bio-Calcium Oxide via Thermal Decomposition of Solid - Hatchery Waste in a Laboratory-Scale Rotary Kiln

A Sustainable, Green-Processed, Ag-Nanoparticle-Incorporated Eggshell-Derived Biomaterial for Wound-Healing Applications

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