This paper includes a discussion of the results of tests concerning changes in the thermal and emission parameters of a boiler fuelled with wood biomass under the influence of air gradation in the combustion process. The test results ensure insight into the combustion process of wood biomass with air gradation, which significantly affected the operation of the device, increasing the mass concentration of the emitted nitrogen oxide (NOx) by combustion temperature lowering, especially in the afterburning zone. The authors observed an increase in the emission of particulate matter (PM) and carbon monoxide (CO) related to the change in the combustion process stoichiometry. The tests were carried out with the use of a heating boiler equipped with an automatic pellet burner. Apart from the mass concentration measurement of the pollution emitted, the tests focused on the measurements of temperature and oxygen levels in the flue gas. The objective of the tests was to confirm the applicability of the air gradation techniques in biomass combustion in order to reduce the emission of harmful substances from heating boilers, which is a technique that has recently been used in this group of devices. The test results obtained confirm the necessity for reorganising the technical systems of the currently used pellet burners and implementing further empirical tests.