Optimizing the use of sex-sorted sperm in timed artificial insemination programs for suckled beef cows1

Filho, Manoel Francisco de Sá; Girotto, R. W.; Abe, E. K.; Penteado, Luciano de Camargo; Filho, E. P. Campos; Moreno, J. F.; Sala, R. V.; Nichi, Marcílio; Baruselli, Pietro Sampaio

doi:10.2527/jas.2011-4523

Cited by 37 publications

(27 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, fertility was lower for cows in which the semen was deposited into the cervix [11]. Similarly, other authors using low doses of semen [10,44] or frozen sex-sorted semen [8,13,45] observed similar fertility level after depositing semen either into the uterine body or uterine horn.…”

Section: Discussionmentioning

confidence: 77%

See 1 more Smart Citation

Effects of deep-horn AI on fertilization and embryo production in superovulated cows and heifers

et al. 2013

View full text Add to dashboard Cite

The primary objective of this study was to determine the effect of site of semen deposition on fertilization rate and embryo quality in superovulated cows. The hypothesis was that deposition of semen into the uterine horns would increase the fertilization rate compared with deposition of semen into the uterine body. The secondary objective was to evaluate the effect of uterine environment on fertilization rate and embryo quality. It was hypothesized that subclinical endometritis at the onset of superstimulation would decrease the fertilization rates and embryo quality. In experiment 1, 17 superovulated heifers were randomly assigned to receive artificial insemination (AI) into the uterine body or uterine horns. The total number of fertilized structures and fertilization rate from superovulated heifers was increased (P = 0.04 and P = 0.02, respectively) when semen was deposited into the uterine horns compared with the uterine body. Other embryo characteristics did not differ based on the site of semen deposition. In experiment 2, 14 lactating dairy cows were superovulated twice and were randomly assigned to receive AI into the uterine body or deep into the uterine horns using a crossover design. Neither fertilization rate nor any other embryo characteristics were improved when semen was placed deep into the uterine horns compared with the uterine body. In experiment 3, 72 superovulated lactating dairy cows were randomly assigned to receive AI into the uterine body or uterine horns. Before initiation of superstimulatory treatments, an endometrial cytology sample was collected from each cow. Ova/embryos were collected by a nonsurgical technique at 70 ± 3 days in milk. Similar to experiment 2, neither fertilization rate nor any other embryo characteristics differed based on the site of semen deposition in experiment 3. The percentage of cows with subclinical endometritis did not differ between treatments. Interestingly, there was a tendency (P = 0.09) for a reduction in embryo recovery rate and a reduction (P = 0.01) in the fertilization rate for cows with subclinical endometritis. In conclusion, deposition of semen into the uterine horns rather than into the uterine body did not improve the fertilization rate or embryo quality in superovulated cows. Subclinical endometritis decreased the fertilization rate in superovulated cows.

show abstract

Section: Discussionmentioning

confidence: 77%

“…Deposition of semen into the uterine horns has been proposed as an alternative method to the traditional deposition into the uterine body. However, comparison of these two techniques has produced surprisingly variable results with reports of deep-horn AI having no effect [7–13] or improving [14–16] fertility in cattle.…”

Section: Introductionmentioning

confidence: 99%

Effects of deep-horn AI on fertilization and embryo production in superovulated cows and heifers

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Perry et al (2008a,b) suggested that preovulatory concentrations of estradiol during standing estrus may modulate uterine pH to affect sperm transport. However, that study (Sá Filho et al, 2012) involved Bos indicus cows rather than Bos taurus cows as in our study. Our data differ somewhat from those of Sá Filho et al (2012), who reported that pregnancy rates following FTAI to sex-sorted semen were not significantly lower than pregnancy rates to conventional semen among cows that had not exhibited estrus by the standard FTAI time.…”

Section: Discussionmentioning

confidence: 97%

“…However, that study (Sá Filho et al, 2012) involved Bos indicus cows rather than Bos taurus cows as in our study. Administration of ECP or EB following progestin device removal in the study by Sá Filho et al (2012) likely provides an enhanced estradiol environment and may potentially alleviate some of the reduction in FTAI pregnancy rates when using sex-sorted semen in nonestrous cows. Jinks et al (2013) demonstrated that administration of ECP to postpartum cows 24 h before GnRHinduced ovulation of a small dominant follicle and FTAI resulted in increased pregnancy rates compared to cows that did not receive ECP.…”

Section: Discussionmentioning

confidence: 97%

Delayed insemination of nonestrous cows improves pregnancy rates when using sex-sorted semen in timed artificial insemination of suckled beef cows1

Thomas

Lock

Poock

et al. 2014

Journal of Animal Science

View full text Add to dashboard Cite

This experiment was designed to test the hypothesis that delayed insemination of nonestrous cows would increase pregnancy rates when using sex-sorted semen in conjunction with fixed-time artificial insemination (FTAI). Estrus was synchronized for 656 suckled beef cows with the 7-d CO-Synch + controlled internal drug release (CIDR) protocol (100 μg GnRH + CIDR [1.38 g progesterone] on d 0, 25 mg PGF2α at CIDR removal on d 7, and 100 μg GnRH on d 10, 66 h after CIDR removal). Estrus detection aids (Estrotect) were applied at PGF2α and CIDR removal on d 7, and estrous expression was recorded at GnRH on d 10. Cows were assigned to 1 of 3 treatments: 1) FTAI (concurrent with GnRH, 66 h after CIDR removal) with conventional semen regardless of estrous expression, 2) FTAI with sex-sorted semen regardless of estrous expression, or 3) FTAI with sex-sorted semen for cows having expressed estrus and delayed AI 20 h after final GnRH for cows failing to express estrus. A treatment × estrous expression interaction was found (P < 0.0001). Higher pregnancy rates (P < 0.0001) were achieved with conventional semen (Treatment 1; 77%) than with sex-sorted semen (Treatments 2 and 3; 51 and 42%, respectively) among cows that expressed estrus. However, among cows that failed to express estrus, delayed insemination with sex-sorted semen yielded higher (P < 0.0001) pregnancy rates than with sex-sorted semen at the standard time (Treatments 2 and 3; 3 versus 36%, respectively). Furthermore, among cows that failed to express estrus, FTAI pregnancy rates when using sex-sorted semen at the delayed time (36%) were comparable (P = 0.9) to those achieved using conventional semen at the standard time (Treatment 1; 37%). These results indicate that delaying AI of nonestrous cows by 20 h from the standard FTAI improves pregnancy rates when sex-sorted semen is used with FTAI.

show abstract

“…Jeśli w programach inseminacji w ustalonym czasie (TAI) pęcherzyk dominujący (PD) był mniejszy od 9 mm, to wskaźnik zacieleń wynosił 31,2%, jeśli był większy od 9 mm -56,8%. Różnice te w odniesieniu do nasienia konwencjonalnego, niezależnie od rozmiarów PD były porównywalne (58). Nie zmienia to faktu, że mimo wykorzystania tych samych kryteriów wyboru krów do inseminacji (pęcherzyk dominujący 12-18 mm, wypływ klarownego śluzu) wyniki zacieleń po inseminacji nasieniem sortowanym były istotnie niższe niż w przypadku użycia nasienia konwencjonalnego (25,7% wobec 39%) (36).…”

unclassified

Sex-sorted sperm in farm animals: scale of use, the effectiveness of insemination, risk factors

Jaśkowski

Woźna

Gehrke

2017

Medycyna Weterynaryjna

View full text Add to dashboard Cite

The aim of the study was to present the scale of use, risk factors and possibilities, which sorter semen gives in biotechnics used in reproduction of cattle. Modern sorters allow for the evaluation of 6 million X and Y spermatozoa per hour. Sex-sorted semen, which is commercially used, contains 2.1 x 106 of spermatozoa. It is used mostly in AI of milk heifers, mainly in large cattle herds. Sorted semen containing Y spermatozoa is sold less often in the world than the one with X spermatozoa. The percentage of the desired sex of the young is higher than 90. The pregnancy rate after application of sorted semen is about 20–25% lower than after insemination of non-sorted semen and depends on a number of factors. The main factors are: breed of female, service number, the herd of origin, the depth of semen deposition, the bull producing semen, ambient temperature and technical parameters during sperm sorting. A number of methods have been developed to improve conception rate, including timed artificial insemination (TAI) and synchronization of heat and ovulation. Results of donor inseminations with the use of sorter semen are presented, with the lower percentage of embryos suitable for the transfer and embryos of the highest quality highlighted. Previous studies do not indicate a reduction of the conception rate after the transfer of embryos obtained in vitro and in vivo after fertilization using sorted semen. It remains difficult to justify a significant increase in the frequency of stillbirths of bulls after using sorted sperm. Similarly, 16% of stillbirths of bulls were observed after embryo transfer, when donors were inseminated with sorter semen. The percentage of stillbirths of bulls after embryo transfer with the use of conventional semen is 9%. The sorted semen is not often used for inseminations in pigs, sheep and goats.

show abstract

Optimizing the use of sex-sorted sperm in timed artificial insemination programs for suckled beef cows1

Cited by 37 publications

References 32 publications

Effects of deep-horn AI on fertilization and embryo production in superovulated cows and heifers

Effects of deep-horn AI on fertilization and embryo production in superovulated cows and heifers

Delayed insemination of nonestrous cows improves pregnancy rates when using sex-sorted semen in timed artificial insemination of suckled beef cows1

Sex-sorted sperm in farm animals: scale of use, the effectiveness of insemination, risk factors

Contact Info

Product

Resources

About