2006
DOI: 10.1007/s00383-006-1727-3
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Optimizing vector application for gene transfer into human hepatoblastoma cells

Abstract: Gene targeting is currently of distinct interest as an innovative additive treatment option in various malignancies. Its role in pediatric liver tumors has not yet been evaluated thoroughly. For the first time the authors systematically analyzed both lipid-based transfection as well as transduction with adenovirus vectors (Ad) and Sendai virus vectors (SeVV) in order to optimize gene transfer into hepatoblastoma (HB) cells. Two HB cell lines were infected with Ad or SeVV coding for green fluorescent protein (A… Show more

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Cited by 3 publications
(2 citation statements)
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“…This approach was associated with lowest cytotoxicity rates of all investigated gene transfer methods. However, transduction rates for liposomal transfection were inferior to those for other systems and this method was thus considered applicable especially for production of stable cell lines [43]. Optimal transduction rates together with low cytotoxicity were observed for E1-/E3-deficient adenovirus vectors [43].…”
Section: Gene Transfer Systems For Hepatoblastomamentioning
confidence: 98%
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“…This approach was associated with lowest cytotoxicity rates of all investigated gene transfer methods. However, transduction rates for liposomal transfection were inferior to those for other systems and this method was thus considered applicable especially for production of stable cell lines [43]. Optimal transduction rates together with low cytotoxicity were observed for E1-/E3-deficient adenovirus vectors [43].…”
Section: Gene Transfer Systems For Hepatoblastomamentioning
confidence: 98%
“…In contrast, Sendai virus vectors produced markably higher rates of cytotoxicity compared with adenovirus-based vectors. Therefore, Sendai virus vectors were considered the most promising for direct oncolytic approaches [43].…”
Section: Gene Transfer Systems For Hepatoblastomamentioning
confidence: 99%