Optimum conditions for the production of lipase by alginate-immobilized bacteria.

Zakaria, Zainoha; Razak, Che Nyonya Abdul; Ampon, Kamaruzaman Hj.; Basri, Mahiran; Yunus, Wan Md Zin Wan; Shirai, Yoshihito; Salleh, Abu Bakar; Hashimoto, Kenji

doi:10.2323/jgam.38.429

Cited by 7 publications

(4 citation statements)

References 18 publications

(13 reference statements)

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“…A curing time of longer than 2 h after bead formation in 0.45 M CaC12 was also regarded as enough time to complete gelation. 19) It has been noted that alginate gels have a large number of pores20) and the pores are related to the diffusion of nutrients and products.17) In our system using 1 % sodium alginate, our data showed that although the immobilized bacteria were maintained in the beads, the antiviral sub stances produced were released efficiently into the medium. However, electron microscopic observation of Pseudomonas sp.…”

Section: Bacterial Culturesmentioning

confidence: 57%

Production of Anti-Infectious Hematopoietic Necrosis Virus (IHNV) Substances by Immobilized Whole Living Cells of Aquatic Bacteria in a Bioreactor System

et al. 1995

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A simple, low-cost and efficient bioreactor system for production of anti-infectious hematopoietic necrosis virus (IHNV) substances by bacteria isolated from aquatic environments was devised on a laboratory scale. Whole living cells of anti-IHNV substances producing bacteria, Pseudomonas sp. 51BBW-29 and Alteromonas sp. 48HS-27, were immobilized in calcium alginate beads prepared by 1% sodium alginate, 0.45M CaCl2, and C2Y or MC2Y broth. Eighteen g of the beads were packed into a 25 ml reaction column and incubated at 15℃ for 5 days in a circulating broth medium at a flow rate of 0.35ml/h. After activation of the immobilized bacteria, the beads were incubated in a continuous flow of fresh broth with intermittent exposure to air. In the beads incubated under these conditions, the immobilized bacteria grew well at the gel surface and high viability of approximately 10^10 CFU/bead was retained for at least 6 days from day 8 to 14. During 31 days of the experiment with the bioreactor system, the highest production was 69.3 ⊿IHD50 for Pseudomonas sp. 51BBW-29 (at 14-17 days incubation) and 48.0 ⊿IHD50 for Alteromonas sp. 48HS-27 (at 8-11 days incubation), which were 2.5 and 2.7 times higher than those of conventional batch culture (27.3 and 18.0 ⊿IHD50 for the two strains at 2-5 days incubation). The immobilized bacteria continuously produced high levels of anti-IHNV substances for 15 days (day 5-20)

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Section: Bacterial Culturesmentioning

confidence: 57%

Production of Anti-Infectious Hematopoietic Necrosis Virus (IHNV) Substances by Immobilized Whole Living Cells of Aquatic Bacteria in a Bioreactor System

et al. 1995

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“…4A). The de‐crosslinking of the alginate‐calcium and then fish oil leakage might have occurred for the capsules stored in a dry state, while only fish oil leakage could have occurred in the capsules stored in a CaCl 2 solution because alginate gels have pores large enough to release any compounds out of the matrix (Zakaria et al ., 1992). The capsules with 20 mg mL −1 alginate concentration could have more alginate crosslinking events with calcium and a lower dehydration rate than capsules with 10 mg mL −1 alginate concentration.…”

Section: Resultsmentioning

confidence: 99%

“…As shown in Fig. 4A, fish oil leakage might occur because alginate gels have large enough pores to release any compounds from the matrix (Zakaria et al ., 1992). In addition, for storage in a dry state, the de‐crosslinking of alginate calcium decreases the weight of every capsule (Fig.…”

Section: Resultsmentioning

confidence: 99%

Shape control and stability of multicore millimetre‐sized capsules using a combined monoaxial dispersion electrospraying–ionotropic gelation technique

Tao

Zhang

Wang

et al. 2021

Int J of Food Sci Tech

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Multicore millimetre-sized fish oil-loaded alginate capsules were developed using a combined monoaxial dispersion electrospraying-ionotropic gelation technique and their stability was explored. By adjusting the preparation parameters, the capsule shapes could be irregular, spherical with a short-tail, spherical, fusiform, and fusiform with a long-tail. The continuous phase of the millimetre-sized capsules consisted of hydrophilic alginate calcium and water. Moreover, the water content increased from 17% to 69% with increased alginate concentration (2.5-30 mg mL À1 ). The capsules prepared with alginate concentration of 10 mg mL À1 or 20 mg mL À1 show a similar loading ratio (about 5-8%) of fish oil during storage. Headspace solid-phase microextraction-gas chromatography mass spectroscopy (HS-SPME-GC-MS) results confirmed the capsules masked the fishy odour of fish oil. Moreover, fish oil slowly migrated from the inside to the outside of the capsules. This work presented a simple method to prepare multicore millimetre-sized capsules with controlled shapes and a basic understanding of the effect of encapsulation using alginate to mask the fish oil odour.

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“…However, a sharp decline in lipase production beyond 200 rpm (1.88 U/ml) was observed. An effi- cient shaking condition has been reported to enhance microbial lipase production as it increases the availability of dissolved oxygen in shake cultures [Zakaria et al, 1992]. Lipase production by a bacterial strain reported by Zakaria et al [1992] was negligible in still culture, but later increased with a shaking rate of up to 200 rpm.…”

Section: Effects Of Physical Factors On Lipase Productionmentioning

confidence: 99%

Enhancement of Thermostable Lipase Production by a Genotypically Identified Extremophilic <i>Bacillus subtilis</i> NS 8 in a Continuous Bioreactor

et al. 2011

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Bacillus strain NS 8, a lipase-producing bacterium isolated from a Malaysian hot spring, is able to tolerate a broad range of temperature and pH, which makes it beneficial for this study. It generated PCR products with molecular weight of 1,532 bp, and the 16S rRNA sequence analysis identified it as Bacillus subtilis with accession number AB110598. It showed a 71% similarity index with B. subtilis using Biolog Microstation System. Its lipase production was optimized using a shake flask system by changing the physical (agitation speed, pH and temperature) and nutritional (nitrogen, carbon and minerals) factors. The most suitable combination of the basal medium for lipase production was 2.5% olive oil (carbon), 1.5% peptone (nitrogen), 0.1% MgSO₄ (mineral) at an optimum temperature of 50°C, pH 7.5 and 150 rpm agitation, giving an enzyme yield of 4.23 U/ml. Statistical optimization using response surface methodology was carried out. An optimum lipase production of 5.67 U/ml was achieved when olive oil concentration of 3%, peptone 2%, MgSO₄·7H₂O 0.2% and an agitation rate of 200 rpm were combined. Lipase production was further carried out inside a 2-liter bioreactor, which yielded an enzyme activity of 14.5 U/ml after 15 h of incubation.

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Optimum conditions for the production of lipase by alginate-immobilized bacteria.

Cited by 7 publications

References 18 publications

Production of Anti-Infectious Hematopoietic Necrosis Virus (IHNV) Substances by Immobilized Whole Living Cells of Aquatic Bacteria in a Bioreactor System

Production of Anti-Infectious Hematopoietic Necrosis Virus (IHNV) Substances by Immobilized Whole Living Cells of Aquatic Bacteria in a Bioreactor System

Shape control and stability of multicore millimetre‐sized capsules using a combined monoaxial dispersion electrospraying–ionotropic gelation technique

Enhancement of Thermostable Lipase Production by a Genotypically Identified Extremophilic <i>Bacillus subtilis</i> NS 8 in a Continuous Bioreactor

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