Optimum Extraction of Polysaccharide fromAreca catechuUsing Response Surface Methodology and its Antioxidant Activity

Abstract

This study was designed to investigate optimum extraction of polysaccharide from seeds of Areca catechu (AUP) and explore its antioxidant activity. Response surface methodology was performed to investigate optimum extraction parameters of AUP. The AUP was precipitated into four crude polysaccharides (AUP20, AUP40, AUP60, AUP80) by different concentrations of ethanol, respectively, then basic characteristics and antioxidant activities of AUPs were assayed. The results indicated optimum process of AUP extraction was as follows: extraction time 1.5 h, ratio of water to raw material 30 mL/g, and extraction two times. Furthermore, the range of AUP molecular weight was approximately 1.84 × 104–4.79 × 105 g/mol. AUPs were composed of mannose, glucose, galactose, and arabinose with different molar ratios, respectively. In particular, our study also indicated AUP80 had the strongest antioxidant activity. Practical Applications There is no report regarding the polysaccharide in seeds of Areca catechu, therefore, it is of great significance to investigate the characteristics and activities of these polysaccharide. A study is necessary to find the optimum extraction conditions to obtain a desirable yield with antioxidant activity for the A. catechu polysaccharide (AUP). Our investigation could provide a scientific basis for the use of polysaccharide in seeds of A. catechu in the future. Collectively, AUPs are promising candidates for developing as natural antioxidants.

Extraction Optimization, Characterization, and Bioactivities of Polysaccharides from Pinelliae Rhizoma Praeparatum Cum Alumine Employing Ultrasound-Assisted Extraction

“…It is widely recognized that the monosaccharide composition is an important factor closely related to the bioactivities of natural polysaccharides [ 51 ]. Monosaccharide composition of PSA was determined by HPLC as previously described with some modifications [ 52 ]. PSA (10 mg) was hydrolyzed by trifluoroacetic acid (TFA, 2 mol/L) at 110 °C for 5 h. Then TFA was removed under reduced pressure, and the residue was washed by methanol for three times before re-dissolving in 2 mL deionized water.…”

Extraction optimization and characterization of polysaccharide antioxidants from <i>Pinellia ternata</i> (Thunb) Breit rhizome

“…[15]. Polysaccharides extracted from natural materials have been reported to have significant antioxidant activities based on large amounts of antioxidant assays in vitro and in vivo [29].…”

“…Standard monosaccharides (Mannose, Glucose, Galactose, Galactose acid, and Arabinose), and dextrans of different molecular weights were obtained from National Crude polysaccharide extraction was carried out using a method described previously [15] with some modifications. The Pinellia Rhizoma (PR) powder was extracted with 95 % ethanol (1 : 3, w/v) for 2 h by reflux.…”

Effect of polysaccharide from the root of <i>Bupleurum Chinese</i> DC and <i>Bupleurum scorzonerifolium</i> Willd on hydrogen peroxide-induced myocardial apoptosis

“…It is well-known that polysaccharides are important biological macromolecules isolated from natural products [6]. polysaccharides derived from naturally occurring medicinal plants have been evaluated as drug candidates for treating various diseases due to their widespectrum of therapeutic activities, relative low toxicities, and minor side-effects [7,8]. However, no reports exist on the protective effect of Bupleuri Radix polysaccharide (BRP) on cardiomyocyte cells and its possible mechanisms of action on myocardial cell preservation.…”