The paper presents field experiments and laboratory analyses to study productivity and nutritional qualities of the grain of four F1 hybrids of winter rye (Aviator, Ravo, Prommo, Eterno), and the population variety Chulpan 7 in the natural conditions of the Southern Ural. The research revealed an individual difference in the nutritional qualities of grain and the content of anti-nutritional substances between different studied F1 hybrids, and compared them with the Chulpan 7 variety. Hybrids of winter rye formed higher grain productivity (from 4.65 t/ha to 6.07 t/ha) in comparison with the Chulpan 7 variety. The paper assesses the content of anti-nutritional substances (water-soluble pentosans) in grain, the kinematic viscosity of the water-extractable from grain hybrids (water-soluble pentosans 1.55 ‒ 1.74%, the viscosity of the water extract 18.3 ‒ 23.1 cSt and winter rye variety (of water-soluble pentosans 1.75%, the viscosity of the water extract was 23.4 cSt), and the dependence of these indicators on the environment during the growing season. The paper also presents the results of determining the nutritional value of the grain of hybrids and the Chulpan 7 variety by the content of crude proteins, starch, phosphorus, calcium, and potassium.