In experimental studies, achieving an optimal plot configuration holds significant importance. This study aimed to establish suitable experimental plot sizes for evaluating agronomic traits of clone LB1 conilon coffee seedlings grown in tubes. Both non-destructive and destructive traits were evaluated using seedlings obtained from a nursery, with each seedling allocated to basic experimental units. A completely randomized block design was used and various scenarios were generated based on combinations of treatments (3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35 and 40), replicates (3, 4, 5, 6 and 7) and levels of experimental precision (10, 20, 30 and 40 %). Convenient plot size recommendations were obtained for clone LB1 conilon coffee seedlings grown in tubes.