Optimum separation condition of peptides in reversed-phase liquid chromatography

Lee, Seung Ki; Row, Kyung Ho

doi:10.1016/j.jchromb.2003.09.061

Cited by 4 publications

(1 citation statement)

References 17 publications

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“…It was found that M. biondii contained significantly higher content of magnolin (28), ranging from 1.26 to 1.77% (w/w), than M. denudata (0.007-0.024%) and M. sprengeri (0.016%), while only M. biondii contained fargesin (30) (0.078 -0.129%, w/w) [55]. However, these studies had certain limitations such as poor resolution of the peaks, broadening of late-eluting peaks and a long running time, which might result in misidentification and/or inaccurate quantification [56]. Thus, a gradient elution method was developed, using a RP-C 18 column (ODSII, L 250 4.6 I.D.…”

Section: Chemical Approaches For Analysis Of Flos Magnoliae Speciesmentioning

confidence: 99%

Chemistry and Bioactivity of Flos Magnoliae, A Chinese Herb for Rhinitis and Sinusitis

Shen

Zhou³

et al. 2008

CMC

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Flos Magnoliae (FM, Chinese name: Xin-yi) is one of the most commonly used Chinese medicinal herbs. It has a long history of clinical use for managing rhinitis, sinusitis and headache. More than 20 different FM species have been used clinically, which makes species identification and evaluation of pharmacological effects of individual chemical ingredients difficult. In this review, we have summarized the current knowledge on FM phytochemistry and its bioactivity activities. The bioactive compounds in FM include both lipid and water-soluble components. More than 90% of the essential components of FM species are terpenoids, including monoterpenes and sesquiterpenes. Lignans and neolignans including tetrahydrofurofuran, tetrahydrofuran and aryltetralin are also present in FM species. A small number of water-soluble compounds have been isolated from Magnolia flower buds, including a benzylisoquinoline alkaloid magnoflorine, an ester ethyl-E-p-hydroxyl-cinnamate and a flavonoid biondnoid. A wide range of pharmacological actions of FM have been reported, including anti-allergy, anti-inflammation and anti-microbial activity. The structure-activity relationship analysis revealed the influence of methylation at position 5 on the 3,7-dioxabicyclo-(3,3,0)-octane backbone of six lignans in antagonistic activities against platelet-activating factor. In addition, the trans stereoisomer fargesin had a much lower bioactivity than the cis stereoisomer demethoxyaschantin. Recent studies have been directed towards the isolation of other bioactive compounds. Further studies on FM may help to develop new anti-inflammatory and anti-allergic drugs.

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Section: Chemical Approaches For Analysis Of Flos Magnoliae Speciesmentioning

confidence: 99%

Chemistry and Bioactivity of Flos Magnoliae, A Chinese Herb for Rhinitis and Sinusitis

Shen

Zhou³

et al. 2008

CMC

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Theoretical analysis of chromatographic peak asymmetry and sharpness by the moment method using two peptides

Choi

Row

2004

Biotechnol. Bioprocess Eng.

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Label-free quantification using MALDI mass spectrometry: considerations and perspectives

Benk

Roesli

2012

Anal Bioanal Chem

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Profound knowledge of protein abundances in healthy tissues and their changes in disease is crucial for understanding biological processes in basic science and for the development of novel diagnostics and therapeutics. Mass spectrometrybased label-free protein quantification is used increasingly often to gain insights into physiological changes observed in perturbed systems. Although the soft ionization techniques electrospray ionization and matrix-assisted laser desorption/ionization have both been used for protein quantification, this article focuses on instrumental setups with a MALDI ion source. Beside reviewing current bioinformatic data-processing tools for label-free quantification and elaborating on the technical benefits of combining UHPLC and MALDI-MS, we outline the potential of state-of-the-art instruments by reporting unpublished results obtained from twenty-four complex biological samples. This review points out that the capabilities of LC-MALDI MS systems have not yet been fully utilized because of a lack of suitable software tools.

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Optimum separation condition of peptides in reversed-phase liquid chromatography

Cited by 4 publications

References 17 publications

Chemistry and Bioactivity of Flos Magnoliae, A Chinese Herb for Rhinitis and Sinusitis

Chemistry and Bioactivity of Flos Magnoliae, A Chinese Herb for Rhinitis and Sinusitis

Theoretical analysis of chromatographic peak asymmetry and sharpness by the moment method using two peptides

Label-free quantification using MALDI mass spectrometry: considerations and perspectives

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