Optimum shade enhances growth and 5,7-Dimethoxyflavone accumulation in Kaempferia parviflora Wall. ex Baker cultivars

Abstract

Light is one of the main limiting factors for phenolic compound biosynthesis in plants and shade levels play an important role in the cultivation of medicinal plants. Kaempferia parviflora a popular rhizomatous medicinal plant was investigated for secondary metabolite and plant biomass accumulation in response to shading and cultivar type. Two cultivars KPM (K.parviflora Malysia) and KPT (K.parviflora Thai) were analyzed for growth and secondary metabolite content under four shade levels (0%, 30%, 50%, and 70%). Photosynthetic capability, plant biomass and total flavonoid content were highest both cultivars under 30% shade after 9 months of growth cycle. 0% shade induced stress on plant growth and reduced photosynthetic capability and the flavonoid accumulation in both cultivars. 5,7dimethoxyflavone (DMF) was used as a chemical marker to quantify secondary metabolite content in K. parviflora cultivars. DMF was greatest in KPM grown under 30% shade. KPT had higher biomass than KPM under 30% but lower DMF content. In conclusion, K. parviflora is a semi shade loving plant suitable to grow under 30% shading for high yield and secondary metabolite production.

Keragaan pertumbuhan tanaman honje (Etlingera elatior) pada beberapa level kerapatan naungan

“…Penelitian ini juga menginformasikan jumlah daun antara umur 10-20 MST pada perlakuan naungan 75% berkisar antara 7,5-11,2 helai; sedangkan pada perlakuan naungan 65% lebih banyak yaitu antara 8,2-12,8 helai. Cahaya memengaruhi alokasi biomasa tanaman, peningkatan level naungan akan menurunkan produksi biomasa tanaman (Labrooya et al, 2016), sehingga jumlah daun pada naungan 75% lebih rendah dari naungan 65%. Hal ini membuktikan bahwa tanaman honje mampu beradaptasi dengan baik pada tingkat naungan 65% dari pada naungan 75% (kontrol) dan tanpa naungan (0%).…”

“…Kandungan klorofil memegang peranan penting dalam absorbsi cahaya selama proses fotosintesis (Labrooya et al, 2016). Oleh karena itu peningkatan produksi biomassa di bawah naungan bisa dibuktikan dengan kandungan klorofil yang tinggi.…”

Establishment of a Rapid Micropropagation System for Kaempferia parviflora Wall. Ex Baker: Phytochemical Analysis of Leaf Extracts and Evaluation of Biological Activities

“…Multiple uses of K. parviflora have necessitated its mass collection as a raw material for pharmaceuticals purposes, leading to the depletion of this wild resource and generating pressure on K. parviflora populations [ 20 ]. Therefore, a sustainable cultivation method is needed to prevent the depletion of natural populations of K. parviflora and meet the growing demand from the pharmaceutical market.…”

“…Therefore, a sustainable cultivation method is needed to prevent the depletion of natural populations of K. parviflora and meet the growing demand from the pharmaceutical market. K. parviflora can be propagated using its rhizomes [ 20 , 21 ]. However, the availability of its rhizome is limited because of its use for extracting commercial metabolites and for preparing K. parviflora products.…”

“…In vitro plant regeneration [ 20 , 21 ], microrhizome formation [ 20 , 25 ], and cell suspension-based culturing [ 26 ] of K. parviflora have been reported. Axillary shoot multiplication has been achieved using terminal shoot buds [ 21 ] or rhizomatous buds [ 20 ]. The authors used 35.52 µM of 6-BA (6-Benzyladenine) to obtain maximal shoot production [ 20 , 21 ].…”