Optimum taper length for maximum fluorescence signal from an evanescent wave fiber optic biosensor

Sun, Kailiang; Kapoor, R.

doi:10.1117/12.771414

Cited by 4 publications

(3 citation statements)

References 6 publications

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“…Distinctive from the above‐mentioned FBG and BOTDR techniques, tapered fibers show the most potential of the sensing elements to generate a fluorescent signal using Evanescent Waves (EWs) associated with the propagating mode in optic fibers. The changing magnitude of the EW field is detected by measuring the changes in output power in a tapered fiber‐based sensor (Sun and Kapoor, ). The tapered fiber‐based sensors that employ this type of technique normally inherit many advantages, such as low cost and easy installation, compared with the FBG and BOTDR techniques.…”

Section: Introductionmentioning

confidence: 99%

Optimization of the Geometries of Biconical Tapered Fiber Sensors for Monitoring the Early‐Age Curing Temperatures of Concrete Specimens

Luo

Ibrahim

Ismail

et al. 2013

Computer aided Civil Eng

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In this article, a geometrical optimization procedure using biconical tapered fiber sensors is proposed for monitoring the early‐age curing temperatures of concrete specimens. The geometries of the sensors are theoretically optimized by the ray‐tracing theory. The results of the theoretical analysis show that the performance of the sensors is heavily influenced by Evanescent Waves, which are due to the tunneling rays and are fully escaped by tapering the fiber. The effects of the geometrical parameters, including the taper ratios, taper lengths, and ray launch angles, as well as the surrounding temperatures, on the behavior of the sensors are studied numerically. The numerical results demonstrate that higher performance of the proposed optimized sensors can be achieved by a longer taper length and smaller taper ratio combined with an initial ray launching angle of 0.01 rad. An experimental study on early‐age curing temperature monitoring of concrete specimens with the biconical tapered fiber sensors was carried out. The experimental measurements agree well with the theoretical results.

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Section: Introductionmentioning

confidence: 99%

Optimization of the Geometries of Biconical Tapered Fiber Sensors for Monitoring the Early‐Age Curing Temperatures of Concrete Specimens

Luo

Ibrahim

Ismail

et al. 2013

Computer aided Civil Eng

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“…1 Further theoretical work and experimental results have also demonstrated that a combination of a taper and a constant diameter probe part further enhances the signal. 16,18,19 In this study, we have used such an evanescent wave-induced fluorescence-based combination tapered fiberoptic biosensor (CTFOB) probe. Another limitation of tapered fiber-optic probe is use of small-diameter fibers (<100 μm).…”

Section: Introductionmentioning

confidence: 99%

Development of combination tapered fiber-optic biosensor dip probe for quantitative estimation of interleukin-6 in serum samples

et al. 2010

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Abstract.A combination tapered fiber-optic biosensor (CTFOB) dip probe for rapid and cost-effective quantification of proteins in serum samples has been developed. This device relies on diode laser excitation and a charged-coupled device spectrometer and functions on a technique of sandwich immunoassay. As a proof of principle, this technique was applied in a quantitative estimation of interleukin IL-6. The probes detected IL-6 at picomolar levels in serum samples obtained from a patient with lupus, an autoimmune disease, and a patient with lymphoma. The estimated concentration of IL-6 in the lupus sample was 5.9 ± 0.6 pM, and in the lymphoma sample, it was below the detection limit. These concentrations were verified by a procedure involving bead-based xMAP technology. A similar trend in the concentrations was observed. The specificity of the CTFOB dip probes was assessed by analysis with receiver operating characteristics. This analysis suggests that the dip probes can detect 5-pM or higher concentration of IL-6 in these samples with specificities of 100%. The results provide information for guiding further studies in the utilization of these probes to quantify other analytes in body fluids with high specificity and sensitivity. C 2010 Society of Photo-Optical Instrumentation Engineers.

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“…When compared to small diameter fibers (<100 μm), CTFOB’s (300 μm) are also far more easily handled than similar biosensors being developed, due to the use of combination, rather than continuous tapering, and provide greater re-coupling of surface fluorescence. 1,3 …”

Section: Introductionmentioning

confidence: 99%

Real-time association rate constant measurement using combination tapered fiber-optic biosensor (CTFOB) dip-probes

2010

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This document reports a novel method of measuring association rate constant (ka) for antibody-antigen interaction using evanescent wave-based combination tapered fiber-optic biosensor (CTFOB) dip-probes. The method was demonstrated by measuring association rate constant for bovine serum albumin (BSA) and anti-BSA antibody interaction. “Direct method” was used for detection; goat anti-BSA “capture” antibodies were immobilized on the probe surfaces while the antigen (BSA) was directly labeled with Alexa 488 dye. The probes were subsequently submerged in 3 nM Labeled BSA in egg albumin (1 mg/ml). The fluorescence signal recorded was proportional to BSA anti-BSA conjugates and continuous signal was acquired suing a fiber optic spectrometer (Ocean Optics, Inc.). A 476 nm diode laser was use as an excitation source. Association constant was estimated from a plot of signal as a function of time. Measured association rate constant ka for the binding of BSA with anti-BSA at room temperature is (8.33 ± 0.01) ×104 M−1 s−1.

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Optimum taper length for maximum fluorescence signal from an evanescent wave fiber optic biosensor

Cited by 4 publications

References 6 publications

Optimization of the Geometries of Biconical Tapered Fiber Sensors for Monitoring the Early‐Age Curing Temperatures of Concrete Specimens

Optimization of the Geometries of Biconical Tapered Fiber Sensors for Monitoring the Early‐Age Curing Temperatures of Concrete Specimens

Development of combination tapered fiber-optic biosensor dip probe for quantitative estimation of interleukin-6 in serum samples

Real-time association rate constant measurement using combination tapered fiber-optic biosensor (CTFOB) dip-probes

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