Lodging is an important limiting factor in wheat because it affects growth, yield and grain quality. Plant growth regulators (PGRs) are often used to restrain elongation of internodes, improve lodging traits, and protect yield potentials. An experiment was set up in the greenhouse at the Ottawa Research and Development Centre (ORDC) to study the effect of the selected PGRs (Manipulator, the active ingredient of which is chlormequat; and Palisade, the active ingredient of which is trinexapac-ethyl) on yield, stem height and morphological traits in six spring wheat cultivars (AC Carberry, AAC Scotia, Hoffman, Fuzion, FL62R1, and AW725). Both PGRs reduced plant height and caused a 6% to 48% reduction in the length of the second basal internode. The mixture of the two PGRs had a synergistic affect and made the stem shorter. The application of PGRs significantly reduced lodging, increased stem diameter, thickness, filling degree, and stem strength, and increased leaf relative chlorophyll content. However, application of PGRs significantly reduced grain yield, and the combination of the two PGRs (Manipulator and Palisade) had a synergistic effect and lowered the yield. In general, the effect of Palisade was more evident than that of Manipulator.