2011
DOI: 10.1002/jemt.20890
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Options for determination of 2‐D distribution of collagen fibrils in transmission electron micrographs—Application to the mammalian corneal stroma

Abstract: Methods are presented that will allow for assessment of the nearest neighbors distances and the overall 2-D pattern of collagen fibrils in electron micrographs. The sets of IFDs and their pattern can be compared using simple statistical analyses.

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Cited by 5 publications
(21 citation statements)
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References 43 publications
(71 reference statements)
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“…At a high resolution transmission electron microscopy (TEM), these CFs can be seen to have various diameters (Doughty, 2011). These fibrils of various sizes can be expected to have different properties in terms of strength and/or resistance to physical forces on the tissue in which they can be found (Eyden and Tzaphlidou, 2001; Franchi et al, 2007; Lavagnino et al, 2005; Ottani et al, 2001; Parry et al, 1978).…”
Section: Introductionmentioning
confidence: 99%
“…At a high resolution transmission electron microscopy (TEM), these CFs can be seen to have various diameters (Doughty, 2011). These fibrils of various sizes can be expected to have different properties in terms of strength and/or resistance to physical forces on the tissue in which they can be found (Eyden and Tzaphlidou, 2001; Franchi et al, 2007; Lavagnino et al, 2005; Ottani et al, 2001; Parry et al, 1978).…”
Section: Introductionmentioning
confidence: 99%
“…Following electronic digital capture and download as TIF files, selected TEM images were projected at close to 22 000× magnification. From the projected image, an overlay was prepared of all of the fibril outlines 26,27 . The diameters of fibrils were then measured manually to a final accuracy of close to 10 nm (0.5 mm on the overlays).…”
Section: Methodsmentioning
confidence: 99%
“…5,14 The objective of the present study was to assess whether or not there can be consistency in the 2-D organization of corneal stroma fibrils by applying the same sampling strategy and analysis to a set of micrographs rather than different methods applied to individual examples. 5,14 This was done by taking a representative set of micrographs from carefully matched rabbits with the same region of the corneal stroma being selected for analysis. Therefore, for the first time, a set of pooled data was obtained to allow for assessment of the inter-fibril spacing in the stroma as based on nearest neighbor distances as well as the nearest neighbors peak profile in 2-D radial distribution functions.…”
Section: Introductionmentioning
confidence: 99%
“…5,[10][11][12][13][14] This twodimensional (2-D) descriptor of the organization of collagen fibrils in the cornea can be expected to show a dominant first peak that has been referred to as the nearest neighbors distance. 10 Indirectly, this issue of inter-fibril spacing in the corneal stroma has been considered in other ways as well.…”
Section: Introductionmentioning
confidence: 99%