Options for fertility preservation in prepubertal boys

Abstract

While additional evidence is required to define optimal conditions for ITT cryopreservation with a view to transplantation or IVM, the putative indications for such techniques, as well as their limitations according to disease, are outlined.

Fertility preservation in patients receiving cyclophosphamide therapy for renal disease

“…The goal of FP in patients who have not yet undergone spermatogenesis is preservation of spermatogonial stem cells (SSCs) [33]. SSCs can be isolated from aspirated cell suspension or from cryopreserved immature testicular tissue (ITT).…”

“…SSCs can be isolated from aspirated cell suspension or from cryopreserved immature testicular tissue (ITT). The advantage of ITT cryopreservation is the maintenance of tissue architecture and cell-to-cell contacts between Sertoli cells and germinal stem cells, which eventually become important for the maturation of SSC [33]. Although there has been success with fertilization in animal models, preserved testicular tissue has not yet been demonstrated to be successful for spermatogenesis in humans.…”

Oncofertility in Canada: cryopreservation and alternative options for future parenthood

“…In prepubertal boys, cryopreservation of immature testicular tissue has also shown promise in restoring fertility through germ-cell autotransplantation (after gonadotoxic therapy), testicular tissue xenografting, or in vitro differentiation and maturation of spermatogonial stem cells 84 . However, although fertility restoration has been achieved in animals, research on humans is still required for any of the foregoing methods 94 .…”

New Approaches of Ovarian Tissue Cryopreservation from Domestic Animal Species

“…It is the only available solution for pre-pubertal boys who must receive a gonadotoxic treatment (eg. cancer therapy; (Keros et al, 2005;Keros et al, 2007;Wyns et al, 2007;Wyns et al, 2008;Wyns et al, 2010).…”