Optoacoustic Imaging in Inflammation

Regensburger, Adrian P.; Brown, Emma; Krönke, Gerhard; Waldner, Maximilian J.; Knieling, Ferdinand

doi:10.3390/biomedicines9050483

Cited by 36 publications

(34 citation statements)

References 149 publications

(169 reference statements)

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“…Due to the use of ultrasonic (US) vibrations, which reflect less in tissues than vision, OAI can recognize a wide variety of endogenous or exogenous chemicals within a unified imaging technique while preserving good spatial resolution at a larger depth than optical imaging. OAI can be widely applied in both clinical and experimental contexts without compromising biochemical functions because of its noninvasive character [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

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A Feasible Multimodal Photoacoustic Imaging Approach for Evaluating the Clinical Symptoms of Inflammatory Arthritis

Rajasekar

Nirmala

Bhuvaneswari

et al. 2022

BioMed Research International

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Numerous traditional medical imaging methods, including computed tomography with X-rays, positron emission tomography (PET), and magnetic resonance imaging (MRI), are utilized frequently in medical settings to screen for illnesses, diagnose patients, and track the effectiveness of treatments. When examining bone protrusions, CT is preferred over MRI for scanning connective tissue. Although the picture quality of PET is inferior to that of CT and MR, it is outstanding for detecting the molecular markers and metabolic functions of illnesses. To give high-resolution structural pictures and improved ailment sensitivity and specificity within another image, multimodal data and substantial therapeutic influence on advanced diagnostics and therapeutics have been used. The goal was to evaluate the clinical significance of multimodal photoacoustic/ultrasound (PA/US) articular imaging scoring, a cutting-edge image technique that may show the microvessels and oxygen levels of rheumatoid arthritis-related inflamed joints (RA). The PA/US imaging technology analyzed seven tiny joints. The PA and power Doppler (PD) impulses were semiquantified using a 0–3 grading scale, and the averages of the PA and PD scores for the seven joints are computed. Three PA+SO2 types were found determined by the relative oxygen levels (SO2) measurements of the affected joints. Researchers evaluated the relationships between the disease activity ratings and the PA/US imaging ratings. The PA scores and medical ratings that reflect the extent of the pain have strong relationships with each other, as do the PA+SO2 combinations. PA may be clinically useful in assessing RA. Thus, the research evaluated the clinical symptoms of inflammatory arthritis using a multimodal photoacoustic image process.

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Section: Introductionmentioning

confidence: 99%

“…As a consequence, the PA transmitter range and center frequency are reduced. Consequently, as imaging depths rise, the spatial resolution must diminish over time [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

A Feasible Multimodal Photoacoustic Imaging Approach for Evaluating the Clinical Symptoms of Inflammatory Arthritis

Rajasekar

Nirmala

Bhuvaneswari

et al. 2022

BioMed Research International

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“…To date, imaging of kidney tissue is mostly performed via ultrasound [ 40 ], while imaging techniques such as blood oxygenation level-dependent (BOLD) magnetic resonance tomography still lack significant clinical impact [ 41 , 42 ]. However, due to its scalability in resolution and imaging depth, OAI provides high potential for further developments to visualize kidney parenchyma [ 18 , 43 , 44 ]. In this regard, contrast-enhanced OAI was capable of quantitatively measuring kidney perfusion potentially suitable for determining acute renal injuries and inflammation [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, there is an unmet clinical need for novel imaging biomarkers in pediatric medicine to diagnose and monitor structural, functional, and molecular changes during CKD progression. In this regard, raster-scanning optoacoustic mesoscopy (RSOM) offers the opportunity for non-invasive, agent-free tissue imaging by the photo-/optoacoustic effect [ 17 , 18 ]. Using a pulsed laser light source to excite tissue chromophores such as hemoglobin, RSOM detects acoustic pressure waves generated from thermoelastic expansion of these molecules [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

High-resolution label-free mapping of murine kidney vasculature by raster-scanning optoacoustic mesoscopy: an ex vivo study

et al. 2022

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Background Chronic kidney disease (CKD) is a global burden affecting both children and adults. Novel imaging modalities hold great promise to visualize and quantify structural, functional, and molecular organ damage. The aim of the study was to visualize and quantify murine renal vasculature using label-free raster scanning optoacoustic mesoscopy (RSOM) in explanted organs from mice with renal injury. Material and methods For the experiments, freshly bisected kidneys of alpha 8 integrin knock-out (KO) and wildtype mice (WT) were used. A total of n=7 female (n=4 KO, n=3 WT) and n=6 male animals (n=2 KO, n=4 WT) aged 6 weeks were examined with RSOM optoacoustic imaging systems (RSOM Explorer P50 at SWL 532nm and/or ms-P50 imaging system at 532 nm, 555 nm, 579 nm, and 606 nm). Images were reconstructed using a dedicated software, analyzed for size and vascular area and compared to standard histologic sections. Results RSOM enabled mapping of murine kidney size and vascular area, revealing differences between kidney sizes of male (m) and female (f) mice (merged frequencies (MF) f vs. m: 52.42±6.24 mm2 vs. 69.18±15.96 mm2, p=0.0156) and absolute vascular area (MF f vs. m: 35.67±4.22 mm2 vs. 49.07±13.48 mm2, p=0.0036). Without respect to sex, the absolute kidney area was found to be smaller in knock-out (KO) than in wildtype (WT) mice (WT vs. KO: MF: p=0.0255) and showed a similar trend for the relative vessel area (WT vs. KO: MF p=0.0031). Also the absolute vessel areas of KO compared to WT were found significantly different (MF p=0.0089). A significant decrease in absolute vessel area was found in KO compared to WT male mice (MF WT vs. KO: 54.37±9.35 mm2 vs. 34.93±13.82 mm2, p=0.0232). In addition, multispectral RSOM allowed visualization of oxygenated and deoxygenated parenchymal regions by spectral unmixing. Conclusion This study demonstrates the capability of RSOM for label-free visualization of differences in vascular morphology in ex vivo murine renal tissue at high resolution. Due to its scalability optoacoustic imaging provides an emerging modality with potential for further preclinical and clinical imaging applications.

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“…Taken together, the existing proven capabilities of clinical PAI for the characterization of microvascular abnormalities and inflammatory reactions ( 136 ), suggests the potential for clinical application of the technology to assessment of radiation skin toxicity.…”

Section: Potential Uses Of Photoacoustic Imaging In Radiotherapymentioning

confidence: 99%

The Potential of Photoacoustic Imaging in Radiation Oncology

et al. 2022

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Radiotherapy is recognized globally as a mainstay of treatment in most solid tumors and is essential in both curative and palliative settings. Ionizing radiation is frequently combined with surgery, either preoperatively or postoperatively, and with systemic chemotherapy. Recent advances in imaging have enabled precise targeting of solid lesions yet substantial intratumoral heterogeneity means that treatment planning and monitoring remains a clinical challenge as therapy response can take weeks to manifest on conventional imaging and early indications of progression can be misleading. Photoacoustic imaging (PAI) is an emerging modality for molecular imaging of cancer, enabling non-invasive assessment of endogenous tissue chromophores with optical contrast at unprecedented spatio-temporal resolution. Preclinical studies in mouse models have shown that PAI could be used to assess response to radiotherapy and chemoradiotherapy based on changes in the tumor vascular architecture and blood oxygen saturation, which are closely linked to tumor hypoxia. Given the strong relationship between hypoxia and radio-resistance, PAI assessment of the tumor microenvironment has the potential to be applied longitudinally during radiotherapy to detect resistance at much earlier time-points than currently achieved by size measurements and tailor treatments based on tumor oxygen availability and vascular heterogeneity. Here, we review the current state-of-the-art in PAI in the context of radiotherapy research. Based on these studies, we identify promising applications of PAI in radiation oncology and discuss the future potential and outstanding challenges in the development of translational PAI biomarkers of early response to radiotherapy.

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Optoacoustic Imaging in Inflammation

Cited by 36 publications

References 149 publications

A Feasible Multimodal Photoacoustic Imaging Approach for Evaluating the Clinical Symptoms of Inflammatory Arthritis

A Feasible Multimodal Photoacoustic Imaging Approach for Evaluating the Clinical Symptoms of Inflammatory Arthritis

High-resolution label-free mapping of murine kidney vasculature by raster-scanning optoacoustic mesoscopy: an ex vivo study

The Potential of Photoacoustic Imaging in Radiation Oncology

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