OptoDyCE-plate as an affordable high throughput imager for all optical cardiac electrophysiology

Heinson, Yuli W.; Han, Julie L.; Entcheva, Emilia

doi:10.1016/j.jmccpl.2023.100054

Cited by 6 publications

(1 citation statement)

References 54 publications

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“…The calcium transient as an important analysis method for cardiac function relies heavily on imaging techniques. A low frame rate may affect the temporal and spatial resolution, making it challenging to capture the rapid changes in calcium concentrations accurately and may lead to missed events or incomplete capture of calcium dynamics. , Thus, we did not use these low frame rate videos to analyze calcium waveforms in this work and will use a high frame rate to record videos for calcium transient analysis in the future work. − In addition, in vivo studies investigating conductive patches have shown enhanced functional outcomes, supporting the need for further exploration in vivo . ,,− Before transitioning to in vivo analysis of the PU-rGO membranes for MI treatment, additional research is necessary to optimize the dose, minimize cytotoxicity, and maximize the therapeutic effects of graphene-derived nanoparticle containing materials. ,− …”

Section: Discussionmentioning

confidence: 99%

Reduced Graphene-Oxide-Doped Elastic Biodegradable Polyurethane Fibers for Cardiomyocyte Maturation

Taylor,

Xu,

Rogozinski

et al. 2024

ACS Biomater. Sci. Eng.

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Conductive biomaterials offer promising solutions to enhance the maturity of cultured cardiomyocytes. While the conventional culture of cardiomyocytes on nonconductive materials leads to more immature characteristics, conductive microenvironments have the potential to support sarcomere development, gap junction formation, and beating of cardiomyocytes in vitro. In this study, we systematically investigated the behaviors of cardiomyocytes on aligned electrospun fibrous membranes composed of elastic and biodegradable polyurethane (PU) doped with varying concentrations of reduced graphene oxide (rGO). Compared to PU and PU-4%rGO membranes, the PU-10%rGO membrane exhibited the highest conductivity, approaching levels close to those of native heart tissue. The PU-rGO membranes retained anisotropic viscoelastic behavior similar to that of the porcine left ventricle and a superior tensile strength. Neonatal rat cardiomyocytes (NRCMs) and human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) on the PU-rGO membranes displayed enhanced maturation with cell alignment and enhanced sarcomere structure and gap junction formation with PU-10%rGO having the most improved sarcomere structure and CX-43 presence. hiPSC-CMs on the PU-rGO membranes exhibited a uniform and synchronous beating pattern compared with that on PU membranes. Overall, PU-10%rGO exhibited the best performance for cardiomyocyte maturation. The conductive PU-rGO membranes provide a promising matrix for in vitro cardiomyocyte culture with promoted cell maturation/functionality and the potential for cardiac disease treatment.

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Section: Discussionmentioning

confidence: 99%

Reduced Graphene-Oxide-Doped Elastic Biodegradable Polyurethane Fibers for Cardiomyocyte Maturation

Taylor,

Xu,

Rogozinski

et al. 2024

ACS Biomater. Sci. Eng.

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Optical mapping and optogenetics in cardiac electrophysiology research and therapy: a state-of-the-art review

Baines,

Sha,

Kalla

et al. 2024

Europace

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State-of-the-art innovations in optical cardiac electrophysiology are significantly enhancing cardiac research. A potential leap into patient care is now on the horizon. Optical mapping, using fluorescent probes and high-speed cameras, offers detailed insights into cardiac activity and arrhythmias by analysing electrical signals, calcium dynamics, and metabolism. Optogenetics utilises light-sensitive ion channels and pumps to realise contactless, cell-selective cardiac actuation for modelling arrhythmia, restoring sinus rhythm, and probing complex cell-cell interactions. The merging of optogenetics and optical mapping techniques for ‘all-optical’ electrophysiology marks a significant step forward. This combination allows for the contactless actuation and sensing of cardiac electrophysiology, offering unprecedented spatial-temporal resolution and control. Recent studies have performed all-optical imaging ex vivo and achieved reliable optogenetic pacing in vivo, narrowing the gap for clinical use. Progress in optical electrophysiology continues at pace. Advances in motion tracking methods are removing the necessity of motion uncoupling, a key limitation of optical mapping. Innovations in optoelectronics, including miniaturised, biocompatible illumination and circuitry, are enabling the creation of implantable cardiac pacemakers and defibrillators with optoelectrical closed-loop systems. Computational modelling and machine learning are emerging as pivotal tools in enhancing optical techniques, offering new avenues for analysing complex data and optimising therapeutic strategies. However, key challenges remain including opsin delivery, real-time data processing, longevity and chronic effects of optoelectronic devices. This review provides a comprehensive overview of recent advances in optical mapping and optogenetics and outlines the promising future of optics in reshaping cardiac electrophysiology and therapeutic strategies.

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Paradoxical effects of ZIM3, a CRISPRi effector, on human induced pluripotent stem-cell-derived cardiomyocyte electrophysiology

Han,

Heinson,

Pozo

et al. 2024

PNAS Nexus

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We show that Zim3, when used as Zim3-KRAB-dCas9 effector in interference CRISPR, without any guide RNAs, paradoxically upregulates key cardiac ion channel genes in human induced pluripotent stem-cell-derived cardiomyocytes, iPSC-CMs, responsible for healthy resting membrane potential, repolarization of the action potential and electrical transmission of signals. These were found to yield expected functional enhancements consistent with a more mature iPSC-CM phenotype, with potentially desirable properties.

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OptoDyCE-plate as an affordable high throughput imager for all optical cardiac electrophysiology

Cited by 6 publications

References 54 publications

Reduced Graphene-Oxide-Doped Elastic Biodegradable Polyurethane Fibers for Cardiomyocyte Maturation

Reduced Graphene-Oxide-Doped Elastic Biodegradable Polyurethane Fibers for Cardiomyocyte Maturation

Optical mapping and optogenetics in cardiac electrophysiology research and therapy: a state-of-the-art review

Paradoxical effects of ZIM3, a CRISPRi effector, on human induced pluripotent stem-cell-derived cardiomyocyte electrophysiology

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