Optogenetic Control of Voltage‐Gated Calcium Channels

Ma, Guolin; Liu, Jindou; Ke, Yuepeng; Liu, Xin; Li, Minyong; Wang, Fen; Han, Gang; Huang, Yun; Wang, Youjun; Zhou, Yubin

doi:10.1002/anie.201713080

Cited by 24 publications

(24 citation statements)

References 29 publications

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“…OptoSTIM was used in zebrafish and embryonic stem cells to transiently increase by BL intracellular calcium concentrations, and in the mouse hippocampus to modulate memory formation. In the recent optoRGK strategy, 308 ion flux through voltage-gated calcium channels was controlled by BL-induced recruitment of an RGK GTPase to the plasma membrane via the iLID system for light-activated heterodimerization. 203 Upon BL exposure, the GTPase translocated to the membrane and thereby inhibited ion flux through the calcium channels.…”

Section: Photoreceptors As Actuators In Optogeneticsmentioning

confidence: 99%

“…20 and 24). 124,304,305,307,308 An optogenetic approach 348 was also used to modulate by BL the Ca 2+ conduction through the voltage-dependent Ca V 1.2 channel which is expressed in smooth muscle. Cluster formation of Ca V 1.2 channels allows mutual interactions that modulate the gating dynamics and ion conductivity.…”

Section: Photoreceptors As Actuators In Optogeneticsmentioning

confidence: 99%

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Blue-Light Receptors for Optogenetics

2018

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Sensory photoreceptors underpin light-dependent adaptations of organismal physiology, development, and behavior in nature. Adapted for optogenetics, sensory photoreceptors become genetically encoded actuators and reporters to enable the noninvasive, spatiotemporally accurate and reversible control by light of cellular processes. Rooted in a mechanistic understanding of natural photoreceptors, artificial photoreceptors with customized light-gated function have been engineered that greatly expand the scope of optogenetics beyond the original application of light-controlled ion flow. As we survey presently, UV/blue-light-sensitive photoreceptors have particularly allowed optogenetics to transcend its initial neuroscience applications by unlocking numerous additional cellular processes and parameters for optogenetic intervention, including gene expression, DNA recombination, subcellular localization, cytoskeleton dynamics, intracellular protein stability, signal transduction cascades, apoptosis, and enzyme activity. The engineering of novel photoreceptors benefits from powerful and reusable design strategies, most importantly light-dependent protein association and (un)folding reactions. Additionally, modified versions of these same sensory photoreceptors serve as fluorescent proteins and generators of singlet oxygen, thereby further enriching the optogenetic toolkit. The available and upcoming UV/blue-light-sensitive actuators and reporters enable the detailed and quantitative interrogation of cellular signal networks and processes in increasingly more precise and illuminating manners.

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Section: Photoreceptors As Actuators In Optogeneticsmentioning

confidence: 99%

Section: Photoreceptors As Actuators In Optogeneticsmentioning

confidence: 99%

Blue-Light Receptors for Optogenetics

2018

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“…In the fourth place, despite the initial success in neuronal or nonneuronal modulation via upconversion optogenetics, ion‐selective (e.g., Ca 2+ , Na + or K + ) and cell‐specific manipulations are considerable challenges in biomedical applications. There have been few reports that underpin the possible strategies to address the issues: one way is to genetically engineer novel ChRs or other optogenetic toolboxes for selective ion channel modulation, in which Ca 2+ release‐activated Ca 2+ (CRAC) channel‐based optogenetic platform (Opto‐CRAC) represented promising capability to achieve selective Ca 2+ regulation in vitro and in vivo;[25b,68] another approach is the employment of targeted optogene delivery system, recent progress in cell/tissue‐specific lentiviral infection technique has greatly boosted the optogenetic manipulation in clinical research . In terms of the evolution of ChR‐based optogenetic tools is still on the journey, which would significantly benefit remote ion channels manipulation with higher precision and specificity.…”

Section: Summary and Perspectivesmentioning

confidence: 99%

Near‐Infrared Manipulation of Membrane Ion Channels via Upconversion Optogenetics

Wang

et al. 2018

Adv. Biosys.

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Membrane ion channels are ultimately responsible for the propagation and integration of electrical signals in the nervous, muscular, and other systems. Their activation or malfunctioning plays a significant role in physiological and pathophysiological processes. Using optogenetics to dynamically and spatiotemporally control ion channels has recently attracted considerable attention. However, most of the established optogenetic tools (e.g., channelrhodopsins, ChRs) for optical manipulations, are mainly stimulated by UV or visible light, which raises the concerns of potential photodamage, limited tissue penetration, and high‐invasive implantation of optical fiber devices. Near‐infrared (NIR) upconversion nanoparticle (UCNP)‐mediated optogenetic systems provide great opportunities for overcoming the problems encountered in the manipulation of ion channels in deep tissues. Hence, this review focuses on the recent advances in NIR regulation of membrane ion channels via upconversion optogenetics in biomedical research. The engineering and applications of upconversion optogenetic systems by the incorporation multiple emissive UCNPs into various light‐gated ChRs/ligands are first elaborated, followed by a detailed discussion of the technical improvements for more precise and efficient control of membrane channels. Finally, the future perspectives for refining and advancing NIR‐mediated upconversion optogenetics into in vivo even in clinical applications are proposed.

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“…Aside from CRAC channels, more tools have been recently invented to control the activity of other types of Ca 2+ channels, including TRPC channels [105] and voltage-gated Ca 2+ channels [106]. Nonetheless, existing efforts are almost exclusively directed to engineer ion channel regulatory units or synthesize photo-sensitive chemical modulators.…”

Section: Summary and Future Directionsmentioning

confidence: 99%

CRAC channel-based optogenetics

Nguyen

Lin

et al. 2018

Cell Calcium

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Store-operated Ca² entry (SOCE) constitutes a major Ca influx pathway in mammals to regulate a myriad of physiological processes, including muscle contraction, synaptic transmission, gene expression, and metabolism. In non-excitable cells, the Ca² release-activated Ca² (CRAC) channel, composed of ORAI and stromal interaction molecules (STIM), constitutes a prototypical example of SOCE to mediate Ca entry at specialized membrane contact sites (MCSs) between the endoplasmic reticulum (ER) and the plasma membrane (PM). The key steps of SOCE activation include the oligomerization of the luminal domain of the ER-resident Ca sensor STIM1 upon Ca² store depletion, subsequent signal propagation toward the cytoplasmic domain to trigger a conformational switch and overcome the intramolecular autoinhibition, and ultimate exposure of the minimal ORAI-activating domain to directly engage and gate ORAI channels in the plasma membrane. This exquisitely coordinated cellular event is also facilitated by the C-terminal polybasic domain of STIM1, which physically associates with negatively charged phosphoinositides embedded in the inner leaflet of the PM to enable efficient translocation of STIM1 into ER-PM MCSs. Here, we present recent progress in recapitulating STIM1-mediated SOCE activation by engineering CRAC channels with optogenetic approaches. These STIM1-based optogenetic tools make it possible to not only mechanistically recapture the key molecular steps of SOCE activation, but also remotely and reversibly control Ca²-dependent cellular processes, inter-organellar tethering at MCSs, and transcriptional reprogramming when combined with CRISPR/Cas9-based genome-editing tools.

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Optogenetic Control of Voltage‐Gated Calcium Channels

Cited by 24 publications

References 29 publications

Blue-Light Receptors for Optogenetics

Blue-Light Receptors for Optogenetics

Near‐Infrared Manipulation of Membrane Ion Channels via Upconversion Optogenetics

CRAC channel-based optogenetics

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