Optogenetics for controlling seizure circuits for translational approaches

Ledri, Marco; Andersson, My; Wickham, Jenny; Kokaia, Mérab

doi:10.1016/j.nbd.2023.106234

Cited by 6 publications

(4 citation statements)

References 108 publications

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“…Activation/Inactivation systems. Optogenetics is a more recent technique that facilitates convenient manipulation of cellular function [132][133][134][135][136]. With the use of light, proteins can be utilized to affect the function of the cell.…”

Section: Optogenetics: Optical Tools For Real-time Subcellular Manipu...mentioning

confidence: 99%

Opticool: Cutting-edge transgenic optical tools

Fenelon,

Krause,

Koromila

2024

PLoS Genet

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Only a few short decades have passed since the sequencing of GFP, yet the modern repertoire of transgenically encoded optical tools implies an exponential proliferation of ever improving constructions to interrogate the subcellular environment. A myriad of tags for labeling proteins, RNA, or DNA have arisen in the last few decades, facilitating unprecedented visualization of subcellular components and processes. Development of a broad array of modern genetically encoded sensors allows real-time, in vivo detection of molecule levels, pH, forces, enzyme activity, and other subcellular and extracellular phenomena in ever expanding contexts. Optogenetic, genetically encoded optically controlled manipulation systems have gained traction in the biological research community and facilitate single-cell, real-time modulation of protein function in vivo in ever broadening, novel applications. While this field continues to explosively expand, references are needed to assist scientists seeking to use and improve these transgenic devices in new and exciting ways to interrogate development and disease. In this review, we endeavor to highlight the state and trajectory of the field of in vivo transgenic optical tools.

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Section: Optogenetics: Optical Tools For Real-time Subcellular Manipu...mentioning

confidence: 99%

Opticool: Cutting-edge transgenic optical tools

Fenelon,

Krause,

Koromila

2024

PLoS Genet

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“…Optogenetics has yielded groundbreaking findings in epilepsy research, shedding light on the intricate neural processes underlying seizure activity [ 217 , 218 ]. In animal models of epilepsy, researchers have utilized optogenetics to selectively activate or inhibit specific neuron populations in brain regions implicated in seizure generation [ 219 , 220 ]. One significant discovery is the identification of “seizure hotspots” in the brain, regions with a higher propensity to initiate and propagate seizures.…”

Section: Optogenetics In Epilepsy Researchmentioning

confidence: 99%

“…By optogenetically stimulating these seizure hotspots, researchers have been able to trigger epileptic activity and observe the patterns of seizure propagation in real time. Conversely, inhibiting these regions using optogenetics can prevent seizure development, highlighting the critical role of these brain areas in epileptogenesis [ 219 ]. Additionally, optogenetics has elucidated the role of specific cell types, such as inhibitory interneurons, in controlling network excitability and seizure susceptibility [ 221 ].…”

Section: Optogenetics In Epilepsy Researchmentioning

confidence: 99%

A Comprehensive Review of Emerging Trends and Innovative Therapies in Epilepsy Management

Ghosh,

Sinha,

Ghosh

et al. 2023

Brain Sciences

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Epilepsy is a complex neurological disorder affecting millions worldwide, with a substantial number of patients facing drug-resistant epilepsy. This comprehensive review explores innovative therapies for epilepsy management, focusing on their principles, clinical evidence, and potential applications. Traditional antiseizure medications (ASMs) form the cornerstone of epilepsy treatment, but their limitations necessitate alternative approaches. The review delves into cutting-edge therapies such as responsive neurostimulation (RNS), vagus nerve stimulation (VNS), and deep brain stimulation (DBS), highlighting their mechanisms of action and promising clinical outcomes. Additionally, the potential of gene therapies and optogenetics in epilepsy research is discussed, revealing groundbreaking findings that shed light on seizure mechanisms. Insights into cannabidiol (CBD) and the ketogenic diet as adjunctive therapies further broaden the spectrum of epilepsy management. Challenges in achieving seizure control with traditional therapies, including treatment resistance and individual variability, are addressed. The importance of staying updated with emerging trends in epilepsy management is emphasized, along with the hope for improved therapeutic options. Future research directions, such as combining therapies, AI applications, and non-invasive optogenetics, hold promise for personalized and effective epilepsy treatment. As the field advances, collaboration among researchers of natural and synthetic biochemistry, clinicians from different streams and various forms of medicine, and patients will drive progress toward better seizure control and a higher quality of life for individuals living with epilepsy.

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“… 7 , 8 Recently, optogenetics harnessing light to manipulate neural activities via microbial opsins has emerged as a potent method to decipher sophisticated neural circuitries with subcellular spatial resolution and specificity in targeted cell types. 9 – 11 Nonetheless, the necessity for viral transfection restricts its translation to human subjects. 12 Considering the above-mentioned limitations, photothermal neural stimulation as a noninvasive and nongenetic neuromodulation modality has gained significant interest in fundamental neuroscience research and translational studies.…”

Section: Introductionmentioning

confidence: 99%

Intravital photoacoustic brain stimulation with high-precision

Wang,

Zhou,

et al. 2024

J. Biomed. Opt.

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. Significance Neural regulation at high precision vitally contributes to propelling fundamental understanding in the field of neuroscience and providing innovative clinical treatment options. Recently, photoacoustic brain stimulation has emerged as a cutting-edge method for precise neuromodulation and shows great potential for clinical application. Aim The goal of this perspective is to outline the advancements in photoacoustic brain stimulation in recent years. And, we also provide an outlook delineating several prospective paths through which this burgeoning approach may be substantively refined for augmented capability and wider implementations. Approach First, the mechanisms of photoacoustic generation as well as the potential mechanisms of photoacoustic brain stimulation are provided and discussed. Then, the state-of-the-art achievements corresponding to this technology are reviewed. Finally, future directions for photoacoustic technology in neuromodulation are provided. Results Intensive research endeavors have prompted substantial advancements in photoacoustic brain stimulation, illuminating the unique advantages of this modality for noninvasive and high-precision neuromodulation via a nongenetic way. It is envisaged that further technology optimization and randomized prospective clinical trials will enable a wide acceptance of photoacoustic brain stimulation in clinical practice. Conclusions The innovative practice of photoacoustic technology serves as a multifaceted neuromodulation approach, possessing noninvasive, high-accuracy, and nongenetic characteristics. It has a great potential that could considerably enhance not only the fundamental underpinnings of neuroscience research but also its practical implementations in a clinical setting.

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Optogenetics for controlling seizure circuits for translational approaches

Cited by 6 publications

References 108 publications

Opticool: Cutting-edge transgenic optical tools

Opticool: Cutting-edge transgenic optical tools

A Comprehensive Review of Emerging Trends and Innovative Therapies in Epilepsy Management

Intravital photoacoustic brain stimulation with high-precision

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