Optomechanical properties of cancer cells revealed by light-induced deformation and quantitative phase microscopy

Kastl, Lena; Budde, Björn; Isbach, Michael; Rommel, Christina; Kemper, Björn; Schnekenburger, Jürgen

doi:10.1117/12.2184764

Cited by 1 publication

(2 citation statements)

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“…Moreover, hemoglobin mass changes of red blood cells were proposed as parameters to quantify cell quality during storing (12) and image processing based methods were demonstrated to be capable to distinguish between different types of blood cells (13,14). In addition, absolute biophysical parameters such as cell volume and refractive index are highly relevant parameters in recently developed methods for optical cell manipulation (25)(26)(27). Recently, by analysis of suspended blood cells it has been shown that quantitative phase contrast allows to quantify label-free the influence of malaria (18) and salmonella (19) mediated infections, the differentiation of immune cells from stem cells to neutrophils and macrophages (20) as well as the influence of inhibitors and cytokines like mitomycin c and epidermal growth factor (EGF) on cancer cells (21).…”

Section: Introductionmentioning

confidence: 99%

“…It also has been demonstrated that the evaluation of quantitative phase images which are retrieved from adherent cells, for example, by image segmentation-based algorithms, enables reliable cell counting (22), quantification of the cell culture confluence degree (23) as well as continuous label-free proliferation monitoring by observation of the temporal dry mass development (17,21,24). In addition, absolute biophysical parameters such as cell volume and refractive index are highly relevant parameters in recently developed methods for optical cell manipulation (25)(26)(27).…”

Section: Introductionmentioning

confidence: 99%

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Quantitative phase imaging for cell culture quality control

Kastl¹,

Isbach²,

Dirksen

et al. 2017

Cytometry Pt A

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The potential of quantitative phase imaging (QPI) with digital holographic microscopy (DHM) for quantification of cell culture quality was explored. Label-free QPI of detached single cells in suspension was performed by Michelson interferometer-based self-interference DHM. Two pancreatic tumor cell lines were chosen as cellular model and analyzed for refractive index, volume, and dry mass under varying culture conditions. Firstly, adequate cell numbers for reliable statistics were identified. Then, to characterize the performance and reproducibility of the method, we compared results from independently repeated measurements and quantified the cellular response to osmolality changes of the cell culture medium. Finally, it was demonstrated that the evaluation of QPI images allows the extraction of absolute cell parameters which are related to cell layer confluence states. In summary, the results show that QPI enables label-free imaging cytometry, which provides novel complementary integral biophysical data sets for sophisticated quantification of cell culture quality with minimized sample preparation. © 2017 International Society for Advancement of Cytometry.

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