Oral administration of a chymotrypsin-labile peptide--a new test of exocrine pancreatic function in man (PFT).

Abstract

SUMMARY One gram N-benzoyl-L-tyrosyl PABA was orally administered to 24 controls, 15 patients with chronic exocrine pancreatic disease, 13 patients after an attack of acute pancreatitis, two patients with gluten-sensitive enteropathy, and 10 patients with biliary tract disease, peptic ulcer, or other pathology of the gastrointestinal tract. In the presence of chymotrypsin, PABA is split from the peptide and excreted in the urine. The amount of PABA excreted serves as a parameter of exocrine pancreatic function. In 51 patients, exocrine pancreatic secretion was also assessed by the Lundh test. In the control group a mean of 59-6 12-2% (mean ± 2 SD) of the peptide-PABA was excreted over a period of six hours. PABA excretion in exocrine pancreatic deficiency was significantly less (p < 0.001) than in controls. (Fig. 1). In the small bowel, in the presence of chymotrypsin, the peptide is split resulting in the liberation of PABA, which is rapidly absorbed from the gut, undergoes conjugation in the liver, and is excreted in the urine. The amount of PABA recovered in the urine during a certain time is used as an index of exocrine pancreatic function. The test has been used for the assessment of exocrine pancreatic deficiency in protein deficient patas monkeys

The Oral Pancreatic Function Test with N-Benzoyl-L-tyrosyl-p-aminobenzoic Acid: Acute Toxicity and Effects of Renal Function on this Test

“…The leukocyte count never changed significantly during the course of the investigation. These data confirm the previous observations of our and Bornschein's group (7,8) revealing no toxicity of N-benzoyl-L-tyrosyl-p-aminobenzoicacid.…”

“…The results were later confirmed in monkeys with metabolic-pancreatic function test has also been applied to humans ally induced pancreatic insufficiency (2). The test is since that time (3), and on the basis of available reports based on the chymotrypsin-specific cleavage of the it seems capable of fulfilling the expectations suggested orally administered peptide, N-benzoyl-i-tyrosyl-pby a screening procedure (4)(5)(6)(7)(8). The test is generally aminobenzoic acid.…”

“…The p-aminobenzpic acid liberated well tolerated. Till now no toxic side effects have been 0340-076X/80/0018-0551 $02.00 © by Walter de Gruyter & Co. · Berlin -New York found (7). However, a systematic toxicity study has not previously been reported in detail (3)(4)(5)(6)(7)(8).…”

Somatostatinoma syndrome accompanied by overproduction of pancreatic polypeptide.

“…Pancreatic juice was collected at a constant rate for 60 min from the initiation of an infusion of secretin, and analysed for volume, mean bicarbonate concentration and amylase output (Tatebe et al 1976). Pancreatic function diagnostant (PFD) test was carried out using N-benzol-l-tyrosyl-p-aminobenzoic acid (BT-PABA, 0.5 g) and expressed as percent urinary extraction of PABA (Gyr 1976;Miyakawa et al 1980). Normal range is 75 to 100%.…”

The Effect of Exocrine Pancreatic Function on Chloramphenicol Pharmacokinetics in Patients with Cystic Fibrosis

“…Exocrine pancreatic insufficiency was confirmed in each study patient by the use of N-PABA (7,8). Patients discontinued their usual PER therapy for 24 h and were evaluated after an overnight fast.…”