Oral administration of a dominant T-cell determinant peptide inhibits allergen-specific TH1 and TH2 cell responses in Cry j 2–primed mice☆☆☆★

Hirahara, Kazuki; Sakai, Saburo; Serizawa, Nobufusa; Sasaki, Reiko; Sakaguchi, Masahiro; Inouye, Sakae; Taniguchi, Yoshifumi; Kaminogawa, Shuichi; Shiraishi, Akio

doi:10.1016/s0091-6749(98)70334-3

Cited by 64 publications

(46 citation statements)

References 35 publications

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“…Two major T cell epitopes, KQVTIRIGCKTSSS (residues 277-290 of Cry j I) and RAEVSYVHVNGAKF (residues 246-259 of Cry j II) (15,16), named Crp-1 and -2, respectively, were inserted into variable regions in acidic and basic subunits of glycinin A1aB1b (29,30). Fifteen amino acid residues (residues 293-307 of A1aB1b) in the acidic subunit and eight amino acid residues (residues 488-495 of A1aB1b) in the basic subunit were substituted by the Crp-1 and -2 T cell epitopes, respectively, resulting in the recombinant protein A1aB1b-Crp-1 and -2.…”

Section: Methodsmentioning

confidence: 99%

“…Two major allergens, designated Cry j I and Cry j II, were isolated from the pollen (9)(10)(11)(12)(13), and multiple domains of T cell epitope for humans and mice were identified from them (14)(15)(16). It has been reported that oral feeding to mice of a chemically synthesized major T cell epitope peptide of Cry j II reduces levels of Cry j II-specific IgE and IgG antibody responses via a decrease in the production of allergy-associated IL-4 in mice (15). These results open new possibilities for the development of allergen peptide-based immunotherapy for the control of Japanese cedar-induced pollinosis.…”

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confidence: 99%

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A rice-based edible vaccine expressing multiple T cell epitopes induces oral tolerance for inhibition of Th2-mediated IgE responses

Takagi

Hiroi

Yang

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

206

137

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Peptide immunotherapy using multiple predominant allergen-specific T cell epitopes is a safe and promising strategy for the control of type I allergy. In this study, we developed transgenic rice plants expressing mouse dominant T cell epitope peptides of Cry j I and Cry j II allergens of Japanese cedar pollen as a fusion protein with the soybean seed storage protein glycinin. Under the control of the rice seed storage protein glutelin GluB-1 promoter, the fusion protein was specifically expressed and accumulated in seeds at a level of 0.5% of the total seed protein. Oral feeding to mice of transgenic rice seeds expressing the T cell epitope peptides of Cry j I and Cry j II before systemic challenge with total protein of cedar pollen inhibited the development of allergen-specific serum IgE and IgG antibody and CD4 ؉ T cell proliferative responses. The levels of allergen-specific CD4 ؉ T cell-derived allergy-associated T helper 2 cytokine production of IL-4, IL-5, and IL-13 and histamine release in serum were significantly decreased. Moreover, the development of pollen-induced clinical symptoms was inhibited in our experimental sneezing mouse model. These results indicate the potential of transgenic rice seeds in production and mucosal delivery of allergen-specific T cell epitope peptides for the induction of oral tolerance to pollen allergens.Japanese cedar pollinosis ͉ peptide immunotherapy ͉ seed-specific expression

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

A rice-based edible vaccine expressing multiple T cell epitopes induces oral tolerance for inhibition of Th2-mediated IgE responses

Takagi

Hiroi

Yang

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

206

137

View full text Add to dashboard Cite

show abstract

“…Efficacy by oral administration was first demonstrated in a mouse model with a T-cell epitope peptide derived from cedar pollen Cry j 2. 70 Clinical trials have been performed with peptides from the cat major allergen (Fel d 1) and bee venom PLA (Api ml) allergens. When evaluated as vaccines for subcutaneous immunotherapy of allergic patients, Tr1-type allergen-specific immune responses were induced, together with decreased production of the Th2-type cytokines IL-4, IL-5 and IL-13.…”

confidence: 99%

Seed-based oral vaccines as allergen-specific immunotherapies

Takaiwa

2011

Human Vaccines

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“…For the Cj pollinosis, two enzymes (Cry j 1 and Cry j 2) present in Cj pollen are considered the major allergens (10), and several immunodominant human T cell epitopes were determined and evaluated using blood samples from Cj pollinosis patients (11)(12)(13). Peptides comprised of multiple T cell epitopes administered orally effectively reduced allergic responses represented by allergen-specific antibody responses, T cell proliferation, and number of sneezing events (14)(15)(16)(17). Oral administration of protein/peptide drugs including edible vaccines is needle free and convenient and utilizes the whole length of the gastrointestinal tract; however, dosage and vaccine-food interactions are the concerns (18).…”

Section: Introductionmentioning

confidence: 99%

Transcutaneous Peptide Immunotherapy of Japanese Cedar Pollinosis Using Solid-in-Oil Nanodispersion Technology

et al. 2015

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Abstract. Peptide immunotherapy is an attractive approach to relieve allergic symptoms such as rhinitis and asthma. Treatment of Japanse cedar pollinosis (Cryptomeria japonica; Cj), from which over one quarter of Japanese population suffer, is becoming a great concern. Recently, oral feeding of a peptide (7crp) consisting of seven immunodominant human T cell epitopes derived from two enzymes present in Cj pollen was demonstrated to have a benefit in treating Cj pollinosis. In this work, we aimed to apply a novel transcutaneous administration system as a simple and easy peptide delivery for an immunotherapy using a T cell epitope peptide. A modified 7crp peptide (7crpR) which contained triarginine linkers between each epitopes was designed to increase water solubility and was encapsulated in a unique solid-in-oil (S/O) nanodispersion. The S/O nanodispersion consists of a nano-sized peptide-surfactant complex dispersed in an oil vehicle. The S/O nanopartilces having an average diameter of 230 nm facilitated the permeation of the peptide 7crpR into the skin and suppressed serum total IgE and antigen-specific IgE levels in a Cj pollinosis mouse model. Transcutaneous administration of the T cell epitope peptide using the S/O nanodispersion system has potential for future simple and easy immunotherapy of Cj pollinosis.

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Oral administration of a dominant T-cell determinant peptide inhibits allergen-specific TH1 and TH2 cell responses in Cry j 2–primed mice☆☆☆★

Cited by 64 publications

References 35 publications

A rice-based edible vaccine expressing multiple T cell epitopes induces oral tolerance for inhibition of Th2-mediated IgE responses

A rice-based edible vaccine expressing multiple T cell epitopes induces oral tolerance for inhibition of Th2-mediated IgE responses

Seed-based oral vaccines as allergen-specific immunotherapies

Transcutaneous Peptide Immunotherapy of Japanese Cedar Pollinosis Using Solid-in-Oil Nanodispersion Technology

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