Oral Administration of Alginate Oligosaccharide from Padina sp. Enhances Tolerance of Oxygen Exposure Stress in Zebrafish (Danio rerio)

Abstract

Alginate is rich in bioactive compounds and has been known to act as a stimulator on the innate immune system. The objective of this study is to determine polysaccharide and oligosaccharide alginate yield, that percentage inhibition with a different type of extraction, to evaluate growth performance as well as immune response by oxygen stress tolerance. Thermal heating with oven laboratory at 140oC for 4.5 hr was done to breakdown the polysaccharide into oligosaccharide. The extraction was conducted by maceration, filtration, precipitation, and centrifugation. Factorial design with two factors was implemented to 260 Zebrafish and reared in thirteen aquariums (20 fish per aquarium) for 12 days. Zebrafish was fed at different dose (4.0g; 6.0g; 8.0g.kg-1) and different type of extraction [noEDTA/noKCl; KCl; EDTA and (EDTAandKCl)]. The evaluation of radical scavenging activity was done spectrophotometrically at 515 nm. Results showed that the highest alginate yield either polysaccharide or oligosaccharide was gained from KCL treatments, percentage inhibition (82.61%), growth performance as well as tolerance of stress (P<0.05). The best growth performance was reached in oligosaccharide supplementation at 6.0g.kg-1 treatment. It can be concluded that alginate oliogosaccharide produced by thermal heating enhanced the antioxidant activity, boost the fish’s immune system, proofed by better growth performance and more tolerant to the low oxygen stress.

Alginate from Sargassum sp. Improve the Hematology Performance and Oxygen Tolerance Exposure of Lates calcarifer

“…The treatment of unoxygen exposure in L. calcalifer exhibits that oral supplementation of alginate in feed resulted better survival rate compared to the control. Yudiati et al (2020) reported the similar result with low molecular weight sodium alginate in Zebrafish (Danio rerio), and Sudaryono et al (2018) with Sargassum sp. powder feed supplementation in Litopenaeus vannamei.…”

Preliminary Study of Polysaccharide and Oligosaccharide Alginate (AOS) as Prebiotic of Probiotic Bacteria

“…Research from Yudiati et al, (2016; and Lee at al., (2009) clearly described that alginate is an effective supplement to suppress replication of severe pathogenic bacteria and White Spot Syndrome Virus Disease by improving the shrimp immune system and transcription of immune gene expression. Alginate oligosaccharide which produces form thermal heating (Yudiati et al, 2018) also enhanced the tolerance of Zebrafish in oxygen exposure tests (Yudiati et al, 2020).…”

Producing Active Secondary Metabolite Against Pathogenic Vibrio spp. by Actinobacteria-Sodium Alginate Co-Culture

“…(Yudiati and Isnansetyo, 2017) and Padina sp. (Yudiati et al, 2020). Alginate has been shown to the increase non-specific immunity of shrimp (Yudiati et al, 2016;Yudiati et al, 2019) and is capable of improving the resistance to diseases such as V. parahaemolyticus infection (Cheng and Yu, 2013), V. harveyi (Jiang et al, 2017), V. alginolyticus (Cheng et al, 2004;Cheng et al, 2005;Cheng et al, 2007;Cheng et al, 2008), Streptococcus agalactiae (Van Doan et al, 2016), S. iniae (Harikrishnan et al, 2011), White Spot Syndrome Virus (WSSV) (Yudiati et al, 2019), Streptococcus sp.…”