Oral administration of allergen extracts from Dermatophagoides farinae desensitizes specific allergen-induced inflammation and airway hyperresponsiveness in rats

Xie, Qiang-min; Wu, Ximei; Wu, Huimin; Deng, Yifeng; Zhang, Shui-juan; Zhu, Jianping; Dong, Xiaopeng

doi:10.1016/j.intimp.2008.07.015

Cited by 19 publications

(13 citation statements)

References 31 publications

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“…The lung function was assessed 30 min before and after LTB 4 i.c.v on day 21 after sensitization as described previously [14]. Briefly, each anaesthetized rat was placed supine inside a Plexiglas whole-body plethysmograph.…”

Section: Methodsmentioning

confidence: 99%

Exhaled carbon monoxide in asthmatics: a meta-analysis

Zhang

Yao

et al. 2010

Respir Res

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BackgroundThe non-invasive assessment of airway inflammation is potentially advantageous in asthma management. Exhaled carbon monoxide (eCO) measurement is cheap and has been proposed to reflect airway inflammation and oxidative stress but current data are conflicting. The purpose of this meta-analysis is to determine whether eCO is elevated in asthmatics, is regulated by steroid treatment and reflects disease severity and control.MethodsA systematic search for English language articles published between 1997 and 2009 was performed using Medline, Embase and Cochrane databases. Observational studies comparing eCO in non-smoking asthmatics and healthy subjects or asthmatics before and after steroid treatment were included. Data were independently extracted by two investigators and analyzed to generate weighted mean differences using either a fixed or random effects meta-analysis depending upon the degree of heterogeneity.Results18 studies were included in the meta-analysis. The eCO level was significantly higher in asthmatics as compared to healthy subjects and in intermittent asthma as compared to persistent asthma. However, eCO could not distinguish between steroid-treated asthmatics and steroid-free patients nor separate controlled and partly-controlled asthma from uncontrolled asthma in cross-sectional studies. In contrast, eCO was significantly reduced following a course of corticosteroid treatment.ConclusionseCO is elevated in asthmatics but levels only partially reflect disease severity and control. eCO might be a potentially useful non-invasive biomarker of airway inflammation and oxidative stress in nonsmoking asthmatics.

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Section: Methodsmentioning

confidence: 99%

Exhaled carbon monoxide in asthmatics: a meta-analysis

Zhang

Yao

et al. 2010

Respir Res

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“…Five-micrometer sections were stained with H&E, periodic acid-Schiff (PAS) and Massontrichrome to evaluate the general morphology. To determine the peribronchial cell counts and severity of the infiltration of the inflammatory cells, the assays for the mucus-secreting goblet cells and subepithelial fibrosis were performed based on the 5-point scoring system described in our previous papers [20,24]. The analyses were performed in a blind fashion, and the slides were presented in a random order for each examination.…”

Section: Tissue Processing and Histological Analysismentioning

confidence: 99%

Simvastatin delivery via inhalation attenuates airway inflammation in a murine model of asthma

Dong

Shen

et al. 2012

International Immunopharmacology

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“…Just as anti-inflammatory medicines and immunosuppressive agents may be used to combat inflammation, allergic symptoms to HDM may be relieved by specific immunotherapies 21. Xie reported that oral administration of D. farinae extract significantly reduced goblet cell hyperplasia and eosinophilia in a D. farinae -sensitized allergic rat model 22. However, even though recent studies have emphasized the requirement for specific immunotherapy as an allergen-specific approach, they have not addressed the difficulties encountered in allergen standardization and the risk of inducing life-threatening anaphylactic side effects.…”

Section: Discussionmentioning

confidence: 99%

Production of Egg Yolk Antibodies Specific to House Dust Mite Proteins

Lee

Han

et al. 2014

Yonsei Med J

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PurposeHouse dust mites (HDMs) are an important source of indoor allergens associated with asthma, rhinitis and atopic dermatitis. Chicken immunoglobulin (Ig) Y is known to be a good alternative to mice and rabbit antibody production. In this study, we produced IgYs specific to HDMs and investigated their IgE immunoreactivities.Materials and MethodsTotal IgYs were isolated from the yolks of White Leghorn hens immunized with either Dermatophagoides pteronyssinus or D. farinae protein extract. Control antibodies were separated from the yolks of immunized hens with phosphate buffered saline. IgYs specific to HDMs were analyzed using enzyme-linked immunosorbent assay and Western blotting analysis.ResultsThe concentration of egg IgY specific to D. farinae in an immunized hen increased and the highest achieved was 661.3 ug/mg (per an egg) on day 47, compared with 760 ug/mg IgY specific to D. pteronyssinus on day 16. The D. pteronyssinus or D. farinae-specific IgY was detected by binding of each mite proteins, and their immunoreactivities were elevated dependent of the specific IgY concentration.ConclusionIgY specific to HDMs may be a promising antibody for immunological diagnosis as well as identification of possible resistance relating to HDM allergy.

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Oral administration of allergen extracts from Dermatophagoides farinae desensitizes specific allergen-induced inflammation and airway hyperresponsiveness in rats

Cited by 19 publications

References 31 publications

Exhaled carbon monoxide in asthmatics: a meta-analysis

Exhaled carbon monoxide in asthmatics: a meta-analysis

Simvastatin delivery via inhalation attenuates airway inflammation in a murine model of asthma

Production of Egg Yolk Antibodies Specific to House Dust Mite Proteins

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