Oral administration of hesperidin, a citrus flavonone, in rats counteracts the oxidative stress, the inflammatory cytokine production, and the hepatotoxicity induced by the ingestion of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)

Abstract

The objective of the current study was to investigate the protective effects of hesperidin against oxidative stress, altered cytokines levels and histological changes in rats induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Rats were divided randomly into four equal groups (Control, TCDD, hesperidin and TCDD+hesperidin). TCDD and hesperidin were given by gavage, dissolved in corn oil at doses of 2 /kg/week and 50 mg/kg/day respectively. The blood and tissue samples were taken from all rats on the 60 th day, to be analyzed for the determination of oxidative stress, histological changes and cytokine levels. The results indicated that hesperidin prevented oxidative damage caused by TCDD via decrease lipid peroxidation and increased antioxidant defense systems. It also reversed the histological damage induced by TCDD. Although, TCDD led to a significant increase in TNF-␣ and IL-1␤ levels, hesperidin treatment was able to normalize these values in rats. In conclusion, it was shown that TCDD caused adverse effects as regards cytokine levels, histological alterations and oxidative stress in rats. However, hesperidin treatment mitigated these toxic effects. These results suggest that hesperidin could play a protective role against TCDD toxicity.

Hesperidin: A promising anticancer agent from nature

“…which are involved in tumour progression . Hesperidin (50 mg/kg) treatment reverted the levels of TNF-␣ and IL-1␤ in the carcinogen TCDD treated rats (Bentli et al, 2013). Similarly, administration of Citrus Juices (rich in Hesperidin) in NKK (4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone) induced Lung and colon carcinogenesis in rat and mouse showed down-regulation of mRNA expression of different cytokines (TNF-␣, IL-1␤, IL-6) and inflammatory enzymes (COX-2 and iNOS) and up-regulation of mRNA expression of Nrf2 (Nuclear factor-2) and glutathione S-transferase and quinine reductase which clearly confirmed the anti-inflammatory mechanism of hesperidin against NKK induced cancer (Tanaka et al, 2011).…”

Hesperidin Alleviates Oxidative Stress and Upregulates the Multidrug Resistance Protein 2 in Isoniazid and Rifampicin‐Induced Liver Injury in Rats

“…Hesperidin, a flavanone glycoside, is found abundantly in lemons and oranges . Previous reports have confirmed the antioxidant activity and radical scavenging properties of hesperidin using a variety of assay systems . In addition, Watanabe et al.…”

Naturally Derived Compounds Used for Prevention or Regression of Experimentally Induced Liver Fibrosis

“…They assured that a dose of 50 mg/kg/day HDN for 10 consecutive days reversed the oxidative effects of CCl 4 via a significant reduction of thiobarbituric reactive substances (TBARS) levels and stimulating the antioxidant defense system. Further study has confirmed these results as Bentli et al (2013) who determined that HDN protected the liver against dioxin toxicity and prevented liver injury. In addition, Chen et al (2010) determined that HDN reduced oxidative stress indicators, like ROS and lipid peroxidation, in a dose-dependent manner.…”