Oral Administration of Hyaluronan Reduces Bone Turnover in Ovariectomized Rats

Abstract

The effect of oral hyaluronan (HA) on bone loss in ovariectomized (OVX) 3-month-old rats was measured using serum markers of bone turnover and bone mineral density. OVX rats were administered 1 mg/kg HA (OVX + HA) or phosphate-buffered saline (PBS) (OVX + PBS) by oral gavage (5 days/week for 54 days). Additional controls included sham ovariectomy with PBS gavage (Sham + PBS) and no treatment. Oral administration of HA resulted in approximately 50% (p < 0.05) increases in serum HA. Gel filtration analyses showed this was high molecular weight HA (300-500 kDa). Osteopenia was mild due to the young age of the animals. Thus, ovariectomy resulted in a 30% increase in serum collagen N-terminal telopeptides (p < 0.001), a 20% increase in serum nitrate/nitrite levels (p = 0.05), and a 5-6% decrease in femur bone mineral density/content (p < 0.05). HA gavage blunted the development of osteopenia in this model as determined by preventing the 30% increase in serum collagen N-terminal telopeptide levels (p < 0.001) and by reducing bone mineral content loss from 6 to 4%. These results show that oral supplements of HA (gavage solution, 0.12% solution) significantly reduce bone turnover associated with mild osteopenia in rats.

Functional Calcium Binding Peptides from Pacific Cod (Gadus macrocephalus) Bone: Calcium Bioavailability Enhancing Activity and Anti-Osteoporosis Effects in the Ovariectomy-Induced Osteoporosis Rat Model

“…However, it recovered to 94.48%, 77.59%, 77.87%, and 88.44% in RAL, CaCO 3 , CBP-Ca-L, and CBP-Ca-H groups, respectively. This suggested that CBP-Ca could effectively strengthen the mechanical properties of OVX rats [ 39 , 40 ].…”

No Influence of Exogenous Hyaluronan on the Behavior of Human Cancer Cells or Endothelial Cell Capillary Formation

“…These characteristics contribute to the use of HA in cosmetics, medicines, and foods. Recently, orally administered HA was found to have beneficial functions in processes such as immune regulation, pain suppression, host defense, and bone metabolism (Asari and others ; Hill and others ; Tashiro and others ; Ma and others ). Therefore, polysaccharides have attracted attention as a functional food ingredient.…”

Sialoglycoprotein from Gadous morhua eggs improve high bone turnover activity via down-regulating BMP-2/Smads and Wnt/β-catenin signal pathways

“…Gm-SGP improved the high bone turnover status in OVX rats Postmenopausal osteoporosis is closely characterized with simultaneous excessive increase in the rates of bone formation and bone resorption, leading to accelerated bone loss (Pacifici, 1996). Increasing studies have suggested that OVX-induced higher bone turnover rates can reflected by higher bone formation and resorption markers (Ma et al, 2013). Xia et al reported that Ca-SGP limited osteoporosis by reducing the expression of bone formation markers such as ALP, OCN, PICP, and BMP-2 as well as a bone resorption marker such as TRAP (Xia et al, 2015).…”