Oral Administration of <i>Ajuga decumbens</i> Extract Has a Synergetic Effect with Glucosamine on Cartilaginous Injury in a Rabbit Osteoarthritis Model

Sawada, Yoko; Sugimoto, Atsushi; Fukuda, Kazuro; Kurosawa, Takako; Ogawa, Masao; Osaki, Tomohiro; Minami, Saburo

doi:10.1166/jcc.2014.1078

Cited by 3 publications

(9 citation statements)

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“…In the ADE diet group, the levels of PIICP increased markedly at week 8 compared with the placebo group. ADE has been reported to promote the repair of damaged cartilage (8) and enhance the synthesis of collagen in false aged model rats (7). Based on these findings, ADE may have enhanced the synthesis of collagen, especially type II collagen, in the subjects involved in the present study, as evidenced by the increase of PIICP, which is a type II collagen synthesis marker.…”

Section: Discussionsupporting

confidence: 51%

“…Subscale III (joint flexion/stiffness) was markedly improved in the ADE diet group compared with the placebo group at weeks 8 and 12 after the intervention. Notably, it has previously been reported that ADE extract promotes the repair of articular cartilage in a rabbit cartilage damaged model (8). Furthermore, cartilage defects have been reported to correlate with the disturbance of knee extension during the progression of OA (21,22).…”

Section: Discussionmentioning

confidence: 99%

“…Notably, AD has been indicated to have an anti-inflammatory action in a rat arthritis model (7). Furthermore, AD promotes the osteoblastic differentiation of cultured osteoblasts (7) and the repair of articular cartilage and subchondral bone in a cartilage damaged model (8). However, these beneficial effects on the joint have not been studied in humans.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the efficacy of Ajugaï¿½decumbens extract supplement in individuals with knee discomfort associated with physical activity: A randomized, double‑blind, placebo‑controlled study

et al. 2017

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Abstract. The aim of the present study was to assess the efficacy and safety of the oral administration of Ajuga decumbens extract (ADE) supplement to individuals with knee discomfort associated with physical activity. A randomized, double-blind, placebo-controlled study was conducted using 48 subjects. The subjects were randomly allocated to an ADE diet group (oral administration of ADE-containing diet, n=24) or a placebo group (n=24), and the intervention was conducted for 12 weeks. A total of 22 subjects in the placebo group and 22 subjects in the ADE diet group were assessed to be eligible for assessment of the efficacy of supplement. Knee function was assessed by changes in the scores of the Japanese Knee Osteoarthritis Measure (JKOM) questionnaire and the scores of the Japan Orthopedic Association (JOA) criteria, as well as by analyzing the levels of type II collagen synthesis and degradation biomarkers (procollagen II C-terminal propeptide, cross-linked C-telopeptide of type II collagen, collagen type II cleavage and matrix metalloproteinase-13). Outcomes were measured at the baseline and at 4, 8 and 12 weeks from the start of administration. Subscale II (joint flexion/stiffness) of the JOA criteria was markedly improved in the ADE diet group compared with the placebo group at 8 and 12 weeks during the intervention. Furthermore, in the subgroup analyses using subjects with mild knee discomfort, subscale II (pain/stiffness) and IV (general activities) scores of JKOM were significantly improved (P<0.05) and total JKOM score was markedly improved in the ADE diet group compared with the placebo group at week 8 of the intervention. No adverse effects were identified for the administration of ADE. In conclusion, these observations suggest that the administration of an ADE-containing diet is safe and improves joint function (flexion and stiffness) and general activity in subjects with mild knee discomfort. Therefore, ADE could be a promising candidate as a functional food that is beneficial to joint health.

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Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of the efficacy of Ajugaï¿½decumbens extract supplement in individuals with knee discomfort associated with physical activity: A randomized, double‑blind, placebo‑controlled study

et al. 2017

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“…Ajuga decumbens (AD), a naturally occurring herb that has a history of use as a pain relief medicine in Japan ( 10 ), was selected as the candidate supplement for the treatment of OA. AD extract (ADE) has previously been reported to have preventative effects against osteoporosis ( 10 ), to decrease the number of osteoclasts following subchondral bone damage, and to work synergistically with GlcN to improve cartilaginous injury in a rabbit OA model ( 11 ). 20-hydroxyecdysone, an active component of ADE, has also been demonstrated to decrease the number of osteoclasts following subchondral bone damage in cartilaginous injuries ( 11 ).…”

Section: Introductionmentioning

confidence: 99%

“…AD extract (ADE) has previously been reported to have preventative effects against osteoporosis ( 10 ), to decrease the number of osteoclasts following subchondral bone damage, and to work synergistically with GlcN to improve cartilaginous injury in a rabbit OA model ( 11 ). 20-hydroxyecdysone, an active component of ADE, has also been demonstrated to decrease the number of osteoclasts following subchondral bone damage in cartilaginous injuries ( 11 ). Furthermore, 20-hydroxyecdysone has beneficial effects on epiphyseal cartilage tissue and trabecular bone in ovariectomized rats ( 12 ), and ADE has been reported to induce subchondral bone regeneration ( 11 ).…”

Section: Introductionmentioning

confidence: 99%

Ajugaï¿½decumbens stimulates mesenchymal stem cell differentiation and regenerates cartilage in a rabbit osteoarthritis model

et al. 2018

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In a previous study by our group, Ajuga decumbens extract (ADE) was demonstrated to decrease the number of osteoclasts in subchondral bone and to have a synergistic effect with glucosamine in improving cartilaginous injuries in a rabbit model of osteoarthritis. In the present study, a concentrate of the useful fraction of ADE, termed extra ADE (EADE), which includes higher concentrations of the active component 20-hydroxyecdysone, was evaluated for its efficacy to accelerate the healing of experimental cartilage injury. Cartilage injuries were surgically induced in rabbits by creating three holes; one in the articular cartilage of the medial trochlea and two in the trochlear sulcus of the distal femur. The rabbits were divided into the following four groups (n=3 in each): Control, ADE (0.5 g/kg), low dosage EADE (0.05 g/kg; low EADE) and high dosage EADE (0.5 g/kg; high EADE). ADE and EADE were dissolved in tap water and each dosage was orally administered every day for 3 weeks. At the end of the experimental period, histological analysis indicated that the cartilage matrix was regenerated in the low and high EADE groups. On counting of cells in the histological specimens, it was determined that the mean number of osteoclasts per 100 osteoblasts in subchondral bone was lower in the high EADE group compared with the control group. Furthermore, the results indicated that treatment with EADE (1–100 µg/ml) stimulated chondrogenic differentiation of mesenchymal stem cells and induced proteoglycan production to a greater extent than the control in vitro. EADE treatment (10 and 100 µg/ml) was also observed to significantly attenuate interleukin-1β-induced prostaglandin E2 production in chondrocytes (P<0.05). In summary, the results of the present study suggest that EADE may have greater curative effects on bone injury compared with the currently used therapeutic ADE.

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Pharmacology of Iridoids

Dinda

2019

Pharmacology and Applications of Naturally Occurring Iridoids

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Oral Administration of Ajuga decumbens Extract Has a Synergetic Effect with Glucosamine on Cartilaginous Injury in a Rabbit Osteoarthritis Model

Cited by 3 publications

References 0 publications

Evaluation of the efficacy of Ajugaï¿½decumbens extract supplement in individuals with knee discomfort associated with physical activity: A randomized, double‑blind, placebo‑controlled study

Evaluation of the efficacy of Ajugaï¿½decumbens extract supplement in individuals with knee discomfort associated with physical activity: A randomized, double‑blind, placebo‑controlled study

Ajugaï¿½decumbens stimulates mesenchymal stem cell differentiation and regenerates cartilage in a rabbit osteoarthritis model

Pharmacology of Iridoids

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