Oral administration of Lactobacillus delbrueckii subspecies bulgaricus OLL1073R-1 suppresses inflammation by decreasing interleukin-6 responses in a murine model of atopic dermatitis

Abstract

The oral intake of Lactobacillus spp. can provide beneficial effects to the host by modulating the immune response. Atopic dermatitis (AD) is an allergic inflammatory disease mediated by various immune responses. In this study, we examined the effect of a Lactobacillus strain, Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 (OLL1073R-1), on AD development in a murine model of AD that was developed by the topical application of mite antigen in NC/Nga mice. The oral intake of heat-killed OLL1073R-1 cells inhibited both the development of dermatitis and the elevation of an acute inflammation marker, serum amyloid A. Another bacterial strain, Lactobacillus rhamnosus OLL2984, exerted no inhibitory effects on dermatitis. The oral intake of heat-killed OLL1073R-1 cells also attenuated secretion of IL-6 from lymph node cells in response to mite antigen and reduced IL-6 levels in inflamed tissues, such as auricles. Production of IFN-γ or IL-4 was not influenced by OLL1073R-1 intake. We also found that inhibition of IL-6 signaling by gp130-Fc (a fusion protein consisting of the extracellular portion of glycoprotein 130 fused to the Fc region of human IgG1) markedly decreased the severity of dermatitis in NC/Nga mice. Moreover, secretion of IL-6 by lymph node cells was augmented in NC/Nga mice compared with that in BALB/c mice. These results indicate that IL-6 plays an essential role in the development of dermatitis in the NC/Nga mouse model of AD, and that OLL1073R-1 inhibits dermatitis, at least in part, by suppressing the IL-6 response.

Exopolysaccharide Produced by Lactobacillus paracasei IJH-SONE68 Prevents and Improves the Picryl Chloride-Induced Contact Dermatitis

“…However, the inflammation of the mice model using 2,4,6-trinitro-1-chlorobenzene (TNCB) was improved by the oral administration of the EPS without IgE decrease. Some LAB strains improve the murine dermatitis model in a manner independent of the IgE level [42,43]. Therefore, the hypersensitivity-improving effect of EPS might be different with and without IgE changes, suggesting that each receptor may recognize separately the individual tertiary structure of EPS.…”

Dysbiosis of urinary microbiota is positively correlated with Type 2 diabetes mellitus

“…Lactobacillus is probiotic, and can produce organic acid and hydrogen peroxide that create an inhospitable environment for pathogenic bacteria. Treatment with Lactobacillus can reduce the incidence of UTIs [32], and can reduce FBG, homocysteine, and interleukin-6 which play roles in preventing infection for diabetic patients [33–35]. In addition, the higher proportion of Lactobacillus can increase insulin secretion [36].…”

Lactic Acid Bacteria Protects Caenorhabditis elegans from Toxicity of Graphene Oxide by Maintaining Normal Intestinal Permeability under different Genetic Backgrounds

“…Considering the important contribution of C. elegans to nanotoxicology study 5 , we employed the non-mammalian alternative toxicity assay model of C. elegans as the in vivo assessment system. Considering the multiple beneficial effects of L. bulgaricus on organisms such as suppressing inflammation and gastrointestinal well-being 48 49 , we selected the L. bulgaricus as the testing LAB bacteria. In the present study, we mainly selected the intestinal ROS production and locomotion behavior as the toxicity assessment endpoints to reflect the functions of primary and secondary targeted organs, respectively.…”