Oral and aerosol immunization with viable or inactivated<i>Actinobacillus pleuropneumoniae</i>bacteria: antibody response to capsular polysaccharides in bronchoalveolar lavage fluids (BALF) and sera of pigs

Hensel, Andreas; Pabst, Reinhard; Bunka, S; Petzoldt, K

doi:10.1111/j.1365-2249.1994.tb06236.x

Cited by 18 publications

(1 citation statement)

References 34 publications

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“…Therefore, we recommend the term i MALT: Integrated mucosal lymphoid tissue (Pabst and Brandtzaeg 2020), because there is preferential migration of lymphoid cells, e.g., from the gut wall to the bronchial tract. An example is to protect pigs from the bacterial lung infection by Actinobacillus pleuropneumoniae by giving an oral dose of encapsulated bacteria (Hensel et al 1994;Hensel et al 1996). A surprising finding was a dramatic increase of plasma cells in the bronchiolar lavage (Delventhal et al 1992).…”

Section: Mucosal Immunologymentioning

confidence: 99%

The pig as a model for immunology research

Pabst¹

2020

Cell Tissue Res

189

118

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The pig is an omnivorous, monogastric species with many advantages to serve as an animal model for human diseases. There are very high similarities to humans in anatomy and functions of the immune system, e g., the presence of tonsils, which are absent in rodents. The porcine immune system resembles man for more than 80% of analyzed parameters in contrast to the mouse with only about 10%. The pig can easily be bred, and there are less emotional problems to use them as experimental animals than dogs or monkeys. Indwelling cannulas in a vein or lymphatic vessel enable repetitive stress-free sampling. Meanwhile, there are many markers available to characterize immune cells. Lymphoid organs, their function, and their role in lymphocyte kinetics (proliferation and migration) are reviewed. For long-term experiments, minipigs (e.g., Göttingen minipig) are available. Pigs can be kept under gnotobiotic (germfree) conditions for some time after birth to study the effects of microbiota. The effects of probiotics can be tested on the gut immune system. The lung has been used for extracorporeal preservation and immune engineering. After genetic modifications are established, the pig is the best animal model for future xenotransplantation to reduce the problem of organ shortage for organ transplantation. Autotransplantation of particles of lymphnodes regenerates in the subcutaneous tissue. This is a model to treat secondary lymphedema patients. There are pigs with cystic fibrosis and severe combined immune deficiency available.

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Section: Mucosal Immunologymentioning

confidence: 99%

The pig as a model for immunology research

Pabst¹

2020

Cell Tissue Res

189

118

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Liposaccharides and Capsules of the HAP Group Bacteria

Fenwick

1995

Haemophilus, Actinobacillus, and Pasteurella

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Lymphocyte subsets in bronchoalveolar lavage after exposure to Actinobacillus pleuropneumoniae in pigs previously immunized orally or by aerosol

Pabst

Delventhal

Gebert

et al. 1995

Lung

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Young pigs were immunized with the lung-pathogenic bacterium Actinobacillus (Haemophilus) pleuropneumoniae by aerosol or orally using viable and inactivated bacteria. The cellular changes in the bronchoalveolar lavage (BAL) were studied in repeated lavages after the pigs were infected with live bacteria. The nucleated cells in the BAL were differentiated and lymphocyte subsets determined. There were no major differences between the two routes of immunization or between viable and inactivated bacteria. The immunization induced an increase in all lymphocyte subsets studied and in the appearance of plasma cells and lymphoid blasts. The infection did not cause a further increase except in granulocytes. The lack of a booster-type increase in lymphocytes in the BAL might indicate a different immunologic reaction of the lung or that lymphocytes of the BAL do not represent lung lymphocytes in general. The protective effect of the immunization might be deduced from the increase in lymphocytes after immunization but not from the reaction pattern after infection.

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Oral and aerosol immunization with viable or inactivatedActinobacillus pleuropneumoniaebacteria: antibody response to capsular polysaccharides in bronchoalveolar lavage fluids (BALF) and sera of pigs

Cited by 18 publications

References 34 publications

The pig as a model for immunology research

The pig as a model for immunology research

Liposaccharides and Capsules of the HAP Group Bacteria

Lymphocyte subsets in bronchoalveolar lavage after exposure to Actinobacillus pleuropneumoniae in pigs previously immunized orally or by aerosol

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