Oral and Intraperitoneal Administration of Quercetin Decreased Lymphocyte DNA Damage and Plasma Lipid Peroxidation Induced by TSA In Vivo

Abstract

Our previous study showed that quercetin enhances the anticancer effect of trichostatin A (TSA) in xenograft mice given quercetin intraperitoneally (10 mg/kg, 3 times/week). Herein, we investigate whether quercetin administered orally exerts such an effect and prevents the cytotoxic side effects of TSA. We found that quercetin given orally (20 and 100 mg/kg, 3 times/week) failed to enhance the antitumor effect of TSA although it increased the total quercetin concentration more than quercetin administered intraperitoneally in the plasma. The compound quercetin-3-glucuronide (Q3G) increased the most. However, quercetin administered intraperitoneally increased the total quercetin level in tumor tissues more than oral quercetin. Oral and intraperitoneal administration of quercetin similarly decreased lymphocyte DNA damage and plasma lipid peroxidation level induced by TSA. Furthermore, we found that the enhancing effect of Q3G on the antitumor effect of TSA and the incorporation of Q3G was less than that of quercetin in A549 cells. However, we found that A549 cells possessed the ability to convert Q3G to quercetin. In conclusion, different from quercetin administered intraperitoneally, quercetin administered orally failed to enhance the antitumor effect of TSA because of its metabolic conversion. However, it prevented TSA-induced DNA damage and lipid peroxidation.

Antioxidative effects of flavonoids and their metabolites against hypoxia/reoxygenation-induced oxidative stress in a human first trimester trophoblast cell line

“…Other studies showed that oral administration of quercetin or hesperidin mainly increased plasma concentrations of their metabolites (e.g. Q3G and hesperetin) (Chan et al, 2014;Ting, Yeh, & Lien, 2011;Yamamoto et al, 2013). In addition, studies have also found that the amounts of metabolites recovered were significantly less than the amounts of flavonoid ingested (Erlund et al, 2001;.…”

Aqueous extract of yellow maca (Lepidium meyenii ) improves sperm count in experimental animals but response depends on hypocotyl size, pH and routes of administration

“…Many pharmacological studies show that intraperitoneal route is preferred because it favours a better absorption of active principles (Chan et al., ). In addition, the absorption and metabolism of these through the gastrointestinal tract can affect their concentrations and structures in vivo (Memmott et al., ; Sharma, Gescher, & Steward, ).…”

The influence of quercetin on recognition memory and brain oxidative damage in a ketamine model of schizophrenia

“…The purity of all of the chemical reagents was not less than analytical grade. The dose concentration selection for quercetin and ketamine was based on the literature [28][29][30][31].…”

“…After one week of adaptation, the mice were randomly divided into five groups (n = 8 in each group): Corn oil + Saline, Quercetin 50 + Saline, Corn oil + Ketamine, Quercetin 25 + Ketamine and Quercetin 50 + Ketamine. A ketamine injection was applied in physiological saline at 1 ml/100 g volume [32,33] and oral administration of quercetin was applied in corn oil at 1 ml/100 g volume [29,31]. Corn oil and quercetin (25 or 50 mg/kg/day) were given by orogastric gavage once a day for 21 days.…”