Oral brush biopsy analysis by matrix assisted laser desorption/ionisation-time of flight mass spectrometry profiling – A pilot study

Remmerbach, Torsten Wilhelm; Maurer, Katja; Janke, Sebastian; Schellenberger, Wolfgang; Eschrich, Klaus; Bertolini, Julia; Hofmann, H.; Rupf, Stefan

doi:10.1016/j.oraloncology.2011.02.005

Cited by 14 publications

(20 citation statements)

References 18 publications

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“…27 This study provides an important first step into the development of a pre-clinical microfluidic device, which can be used to provide further understanding into the progression and treatment of solid tumors. It is envisaged that the approach will be extremely powerful in studying the metastatic process as well as allowing therapeutic regimens to be tested on biopsies from primary tumor, permitting therapy to the tailored in terms of specific drug combinations and concentrations on a personalized basis directing the oncologist's choice of first-line therapy.…”

Section: Discussionmentioning

confidence: 99%

A Microfluidic System for Testing the Responses of Head and Neck Squamous Cell Carcinoma Tissue Biopsies to Treatment with Chemotherapy Drugs

et al. 2011

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Tumors are heterogeneous masses of cells characterized pathologically by their size and spread. Their chaotic biology makes treatment of malignancies hard to generalize. We present a robust and reproducible glass microfluidic system, for the maintenance and "interrogation" of head and neck squamous cell carcinoma (HNSCC) tumor biopsies, which enables continuous media perfusion and waste removal, recreating in vivo laminar flow and diffusion-driven conditions. Primary HNSCC or metastatic lymph samples were subsequently treated with 5-fluorouracil and cisplatin, alone and in combination, and were monitored for viability and apoptotic biomarker release 'off-chip' over 7 days. The concentration of lactate dehydrogenase was initially high but rapidly dropped to minimally detectable levels in all tumor samples; conversely, effluent concentration of WST-1 (cell proliferation) increased over 7 days: both factors demonstrating cell viability. Addition of cell lysis reagent resulted in increased cell death and reduction in cell proliferation. An apoptotic biomarker, cytochrome c, was analyzed and all the treated samples showed higher levels than the control, with the combination therapy showing the greatest effect. Hematoxylin- and Eosin-stained sections from the biopsy, before and after maintenance, demonstrated the preservation of tissue architecture. This device offers a novel method of studying the tumor environment, and offers a pre-clinical model for creating personalized treatment regimens.

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Section: Discussionmentioning

confidence: 99%

A Microfluidic System for Testing the Responses of Head and Neck Squamous Cell Carcinoma Tissue Biopsies to Treatment with Chemotherapy Drugs

et al. 2011

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“…None of the patients were at pM1. 12 In eight cases scalpel biopsies were performed without any further surgical intervention; therefore no staging parameter could be obtained. All lesions including hyperkeratosis, oral lichen and ulcer were biopsy proven by conventional cytopathology (Table 3).…”

Section: Resultsmentioning

confidence: 99%

“…Our study demonstrated that peptide fingerprints may be obtained from intact cells by using MALDI-ToF MS, which can be used as a discriminant tool to distin- guish the biological nature of samples based on the analysis of complete spectral patterns. 12 This approach showed excellent sensitivity and specificity in determining the distinction between tumour cells and healthy tissue. We therefore decided to further develop the technique of intact cell peptidome profiling (ICPP) by undertaking a blind study to attempt to differentiate between mucosal lesions and neoplastic mucosal tissue.…”

Section: Discussionmentioning

confidence: 99%

“…We assume that fewer procedural steps result in fewer procedure-dependent errors, leading to good sensitivity and specificity. 12 As a consequence, we also endeavored to simplify the procedure of sample preparation remarkably. Additionally, measurement with the Autoflex II MAL-DI-ToF mass spectrometer was arranged automatically for each spot.…”

Section: Discussionmentioning

confidence: 99%

“…Informed consent was obtained from each subject, according to the declaration of Helsinki (World Medical Association 2008). 12 …”

Section: Methodsmentioning

confidence: 99%

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Oral brush biopsy analysis by MALDI-ToF Mass Spectrometry for early cancer diagnosis

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Dentistry proteomics: From laboratory development to clinical practice

Rezende

Lima

Petriz

et al. 2013

Journal Cellular Physiology

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Despite all the dental information acquired over centuries and the importance of proteome research, the cross-link between these two areas only emerged around mid-nineties. Proteomic tools can help dentistry in the identification of risk factors, early diagnosis, prevention, and systematic control that will promote the evolution of treatment in all dentistry specialties. This review mainly focuses on the evolution of dentistry in different specialties based on proteomic research and how these tools can improve knowledge in dentistry. The subjects covered are an overview of proteomics in dentistry, specific information on different fields in dentistry (dental structure, restorative dentistry, endodontics, periodontics, oral pathology, oral surgery, and orthodontics) and future directions. There are many new proteomic technologies that have never been used in dentistry studies and some dentistry areas that have never been explored by proteomic tools. It is expected that a greater integration of these areas will help to understand what is still unknown in oral health and disease.

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Oral brush biopsy analysis by matrix assisted laser desorption/ionisation-time of flight mass spectrometry profiling – A pilot study

Cited by 14 publications

References 18 publications

A Microfluidic System for Testing the Responses of Head and Neck Squamous Cell Carcinoma Tissue Biopsies to Treatment with Chemotherapy Drugs

A Microfluidic System for Testing the Responses of Head and Neck Squamous Cell Carcinoma Tissue Biopsies to Treatment with Chemotherapy Drugs

Oral brush biopsy analysis by MALDI-ToF Mass Spectrometry for early cancer diagnosis

Dentistry proteomics: From laboratory development to clinical practice

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