The aims of this study were to investigate the knowledge, attitude, and practice of dentists in Upper Egypt with regard to oral cancer (OC). Out of 1,200 licensed dental practitioners list, 424 dentists were randomly selected from three governorates in Upper Egypt (Minia, Assuit, and Sohag). The sample size was calculated using the equation considering the knowledge of dentists about OC, confidence level, and margins of error; then, an additional number of dentists were added to guard against nonresponse. Data were collected by face‐to‐face interview using 44 items divided into four sections; first part demonstrates sociodemographic. The second part concerned with the knowledge about OC clinical presentation and diagnosis (12 multiple‐choice questions) as well as its risk factors (17 close‐end questions). The third part consists of six questions focused on the practice of participants, and finally, the last part measures attitude of dentists. The chi‐square test was used to compare between the two or more proportions. A correlation was used for describing the relationship or association between two mutually numerical dependent variables.
p
< 0.05 was considered to indicate significance. Rate of response was 94.3%. The overall knowledge level in the current study was 31.8%. The awareness about OC risk factors was high especially, smoking tobacco and alcohol consumption. Also, over 80% of dentists identified family history and
human papillomavirus
(HPV) as risk factors. Only 37.5% of participants performed regular screening of oral mucosa, and 26.5% reported lymph‐node examination. Regarding attitude, only one quarter of dentists attended continuing educating programs about OC. A statistically significant relation (
p
< 0.0001) between knowledge level and most demographic variables was detected. There was a fair positive correlation (
r
= 0.47) between practice and knowledge scores. A predominant trouble among dentists in Upper Egypt was regarding OC knowledge and practice. Continues education and training programs are highly recommended.