Oral Candida albicans biotypes in chinese patients with and without oral candidosis

Yan, Xu; Samaranayake, LP

doi:10.1016/0003-9969(94)00196-i

Cited by 15 publications

(23 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since the API 20 C carbohydrate assimilation system has a relatively poor discriminatory power, API ZYM and boric acid sensitivity tests were added to complement the API 20 C profiles (11). Results from previous studies of others (16)(17)(18)(19) have demonstrated that this system serves to further differentiate the biotypes into smaller sub-groups. Using this biotyping system, Tsang et al (17) showed that there are many different sub-strains of oral C. albicans in HIV-infected patients.…”

Section: Discussionmentioning

confidence: 91%

See 1 more Smart Citation

Biotypes of oral Candida albicans isolated from AIDS patients and HIV‐free subjects in Thailand

Teanpaisan

Nittayananta

Chongsuvivatwong

2000

J Oral Pathology Medicine

View full text Add to dashboard Cite

This study was conducted to examine biotypes and antifungal susceptibility patterns of oral Candida albicans isolated from HIV-infected patients, HIV-free patients with candidiasis and healthy subjects. All isolates were biotyped using a typing system based on enzyme profiles, carbohydrate assimilation patterns and boric acid resistance. Thirty-eight biotypes were found amongst 218 oral C. albicans isolates. The major biotype found was A1S, which accounted for 32.6% of all isolates, and this biotype was the most common in all groups. There was a greater variety of biotypes of C. albicans in the HIV-infected group than in the other groups; however, there was no statistically significant difference between the groups. The minimum inhibitory concentrations (MICs) of a total of 118 isolates were determined for amphotericin B and for ketoconazole using the National Committee for Clinical Laboratory Standards (NCCLS) broth macrodilution method and the E-test. When the antifungal susceptibility patterns among the groups were compared, a statistically significant difference was found only with amphotericin B. The median MIC of amphotericin B in the HIV-infected group was higher than in the healthy group (P=0.013, NCCLS method; P=0.002, E-test). However, this difference in sensitivity was not restricted to any sub-type investigated. Our results showed that the biotype patterns of C. albicans isolates that colonize HIV-infected patients are similar to those of HIV-free subjects, and there is no relationship between antifungal susceptibility patterns and the biotypes.

show abstract

Section: Discussionmentioning

confidence: 91%

“…On account of the difficulties with some of these techniques, a standardised, simple and highly specific biotyping system developed by Williamson et al. (11), which is technically undemanding and utilises relatively inexpensive commercially available quality controlled media, has been increasingly used in recent years (16)(17)(18)(19).…”

mentioning

confidence: 99%

Biotypes of oral Candida albicans isolated from AIDS patients and HIV‐free subjects in Thailand

Teanpaisan

Nittayananta

Chongsuvivatwong

2000

J Oral Pathology Medicine

View full text Add to dashboard Cite

show abstract

“…More recently, using the identical system, a total of 335 200 oral C. albicans isolates from a Tanzanian child population could be segregated into five predominant biotypes: J1S (19.5%), A1S (16.0%), J1R (14.5%), A1R (9.5%) and P1R (7.5%) (29). Interestingly, the major biotypes from this distant geographic location were identical to those from a non-immunosuppressed adult Chinese population (28). Most of the biotypes identified in the above-mentioned studies were also found amongst our C. albicans isolates, although J1R (20.31%) and J1S (20.09%) were predominant.…”

Section: Discussionmentioning

confidence: 93%

Phenotypic diversity of oral C. albicans isolated on single and sequential visits in an HIV‐infected Chinese cohort

Samaranayake

Yau

et al. 2003

APMIS

View full text Add to dashboard Cite

HIV-infected individuals maintain multiple oral C. albicans strains over time that are thought to undergo microevolution in terms of both phenotypic and genotypic features. To study this phenomenon, a 12-month prospective study was conducted in a cohort of 16 HIV-infected ethnic Chinese individuals with (A) and without (B) symptoms of oropharyngeal candidiasis to evaluate the phenotype distribution among oral C. albicans isolates during disease progression. Oral rinse samples were obtained and up to five C. albicans colony-forming units were selected per each visit, during the one year period of multiple visits. The isolates were phenotyped using two commercially available biotyping kits, the API 20C system, API ZYM system, and a plate test for resistance to boric acid. A total of 261 C. albicans strains in group A were differentiated into 67 biotypes, while 42 biotypes were seen amongst the 182 isolates from group B. The major biotypes in the two groups were similar and were in decreasing order of prevalence J1R, J1S, J6S, J6R, J2S, K1S, J10R, K1R, and K6R; 48 different biotypes were seen in group A and 24 in group B, with some uniquely represented in each group, leading to a significant association between the prevalence of the biotypes J1S and J2S and symptomatic candidiasis (p<0.05). Taken together this study illustrates the wide phenotypic spectrum of oral C. albicans associated with HIV-infection.

show abstract

“…Interestingly, C. albicans phenotype A1R, which was predominant, has been associated mainly with patients with symptomatic C. albicans infections but not with asymptomatic carriers. This may indicate a higher virulence of this phenotype in comparison with other phenotypes (Xu and Samaranayake, 1995). The genotypic characterization with use of the combination of the two different primers yielded 32 different profiles for the 37 C. albicans strains, demonstrating high genotypic divergence of the isolates.…”

Section: Influence Of Necrotic Root Canal On Strain Selectionmentioning

confidence: 89%

Yeasts in Apical Periodontitis

Waltimo

Şen

Meurman

et al. 2003

Critical Reviews in Oral Biology & Medicine

View full text Add to dashboard Cite

Microbiological reports of apical periodontitis have revealed that yeasts can be isolated from approximately 5-20% of infected root canals. They occur either in pure cultures or together with bacteria. Almost all isolated yeasts belong to the genus Candida, and the predominant species is C. albicans. Pheno- and genotypic profiles of C. albicans isolates show heterogeneity comparable with those of isolates from other oral sites. C. albicans expresses several virulence factors that are capable of infecting the dentin-pulp complex, including dentinal tubules. This causes, consequentially, an inflammatory response around the root apex, which suggests a pathogenic role for this organism in apical periodontitis. Yeasts are particularly associated with persistent root canal infections that do not respond favorably to conservative root canal therapy. This may be due to the resistance of all oral Candida species against a commonly used topical medicament, calcium hydroxide. However, other antimicrobial agents may offer alternative therapeutic approaches and improve the treatment of these persistent cases of apical periodontitis.

show abstract

Oral Candida albicans biotypes in chinese patients with and without oral candidosis

Cited by 15 publications

References 10 publications

Biotypes of oral Candida albicans isolated from AIDS patients and HIV‐free subjects in Thailand

Biotypes of oral Candida albicans isolated from AIDS patients and HIV‐free subjects in Thailand

Phenotypic diversity of oral C. albicans isolated on single and sequential visits in an HIV‐infected Chinese cohort

Yeasts in Apical Periodontitis

Contact Info

Product

Resources

About