Oral Collagen Drink for Antiaging: Antioxidation, Facilitation of the Increase of Collagen Synthesis, and Improvement of Protein Folding and DNA Repair in Human Skin Fibroblasts

Abstract

This work unveils a fish collagen drink for improvement of skin aging. Previous studies frequently discussed the skin aging from the angle of the representative characteristics of collagen loss and the oxidative-induced expression of proteolytic enzymes matrix metalloproteinases (MMPs), but few groups comprehensively investigated the efficacy of oral hydrolyzed collagen for enhancing protein folding and DNA repair as well as improving notable cell behaviors. To delineate the broad perspective on delaying skin aging, we inspected the collagen drink-treated fibroblast cells from the molecular and cellular aspects. The results show that the collagen drink could perform the compact antiaging effects on ROS inhibition, the facilitation of the synthesis of extracellular matrix (ECM) proteins, the increase of mitochondrial activity, and improvement of the gene expression regarding correct protein folding, DNA mismatch repair (MMR) and base excision repair (BER). Although the experimental results are built on the cellular models, we believe that the positive outcomes can provide more details on the influence of oral hydrolyzed collagen supplement for antiaging. In short, we have successfully proved that the synergistic effect of the collagen drink could not only reduce the oxidative damage but also ameliorate the cell functionality to compensate the harmful effects induced by UVA.

Antiaging Potential of Peptides from Underused Marine Bioresources

“…Human skin fibroblasts pretreated with 0.125%, 0.25% or 0.5% fish collagen for 24 h were UV-irradiated (10 J/cm 2 ) for 24 h, and cell viability was restored by 7.9%, 22.3% and 28.1%, respectively. Moreover, mitochondrial activity was restored by 30.6%, 16.4% and 36.1%, respectively [ 51 ]. In another cell assay carried out by Han and coworkers, UV irradiation decreased the survival rate of HaCaT cells, illustrated by a total apoptosis rate of 66.8 ± 1.27%.…”

“…After 24 h UV treatment (10 J/cm 2 ), collagen peptides from the hydrolysis of fish collagen protein restore the expression levels of CCT2, CCT5, CCT6A, CCT8, and PINK1 genes to normal levels. The collagen peptides not only enhanced the expression of elastin and collagen and relieved the damage caused by ROS, but also boosted protein folding and DNA repair, lowering the risk of DNA mutation in UV-treated fibroblasts [ 51 ].…”

Cosmetic Potential of a Recombinant 50 kDa Protein

“…Ageing and environmental factors can lead to dermatological conditions including, but not limited to fine lines, wrinkles, dry skin, excessive pore size, skin dyschromia, reduced elasticity, unwanted hair, skin thinning, purpura, actinic keratosis, pruritus, eczema, acne, rosacea, erythema, telangiectasia, actinic telangiectasia, skin cancer and rhinophyma. Oral collagen supplements/drinks have been shown to improve skin hydration and elasticity for older people [51][52][53][54]. Although there are numerous skincare products on the market to improve skin appearance, many consumers are hesitant to use chemically synthesized products they perceive as being environmentally unfriendly or otherwise unsafe.…”

Fish Waste: From Problem to Valuable Resource

“…Collagen drink improved cell viability of UVA irradiated human skin fibroblast (CCD-966Sk), their mitochondrial activity was restored and reactive oxygen species (ROS) production triggered by UVA treatment was reduced. Moreover, collagen drink promoted the expression of SOD1, SOD2, CAT genes, and improved both collagen and elastin production [ 162 ]. In addition, skin-derived peptides from the sutchi catfish P. hypophthalmus showed matrix metalloproteinase (MMP) inhibitory activity [ 163 , 164 ].…”

“…In addition, skin-derived peptides from the sutchi catfish P. hypophthalmus showed matrix metalloproteinase (MMP) inhibitory activity [ 163 , 164 ]. In particular, Lu et al [ 162 ] found that cod skin gelatin hydrolysates were especially active at 0.1 mg/mL inhibiting MMP-1 expression in fibroblasts irradiated with UVB of 20 mJ/cm 2 . Two peptides, GEIGPSGGRGKPGKDGDAGPK and GFSGLDGAKGD, were isolated from these hydrolysates and found to exhibit a significant inhibition of MMP-1, phosphorylated extracellular signal-regulated kinase (p-ERK) and p-p38.…”