Oral Delivery of a DNA Vaccine Expressing the PrM and E Genes: A Promising Vaccine Strategy against Flavivirus in Ducks

Huang, Juan; Jia, Renyong; Shen, Haoyue; Wang, Mingshu; Zhu, Dekang; Chen, Shun; Liu, Mafeng; Zhao, Xinxin; Wu, Ying; Yang, Qiao; Yin, Zhongqiong; Cheng, Anchun

doi:10.1038/s41598-018-30258-3

Cited by 18 publications

(21 citation statements)

References 59 publications

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“…The virus was propagated in the allantoic cavities of 10-day-old specific pathogenfree (SPF) embryonated duck eggs. Vaccine strain using attenuated salmonella-presented TMUV prM-E (pVAX-SME) was kindly gifted by Huang [20]. (The attenuated Salmonella typhimurium aroA − stain SL7207 (S. typhimurium 2337-65 derivative hisG46 DEL407 [aroA::Tn10 (Tcs)]) used in this attenuated salmonella vaccine was kindly provided by Professor Kai Schulze of the Helmholtz Center for Infection Research (Germany).…”

Section: Virus and Vaccinesmentioning

confidence: 99%

“…In the present study, vaccine strains using attenuated salmonella-presented TMUV prM-E gene [20] and recombinant adenovirus-packaged TMUV E gene with duck IL-2 as the vaccine adjuvant were successfully constructed. We wondered whether the heterologous prime-boost regimen (priming with attenuated salmonella vaccine and boosting with recombinant adenovirus vaccine) was more effective than the homologous prime-boost strategies (priming and boosting with the attenuated salmonella vaccine; priming and boosting with the recombinant adenovirus vaccine).…”

Section: Introductionmentioning

confidence: 99%

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Heterologous prime-boost: an important candidate immunization strategy against Tembusu virus

Pan

Jia

et al. 2020

Virol J

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Background: Tembusu virus (TMUV), a newly emerging pathogenic flavivirus, spreads rapidly between ducks, causing massive economic losses in the Chinese duck industry. Vaccination is the most effective method to prevent TMUV. Therefore, it is urgent to look for an effective vaccine strategy against TMUV. Heterologous prime-boost regimens priming with vaccines and boosting with recombinant adenovirus vaccines have been proven to be successful strategies for protecting against viruses in experimental animal models. Methods: In this study, heterologous and homologous prime-boost strategies using an attenuated salmonella vaccine and a recombinant adenovirus vaccine expressing prM-E or the E gene of TMUV were evaluated to protect ducks against TMUV infection for the first time, including priming and boosting with the attenuated salmonella vaccine, priming and boosting with the recombinant adenovirus vaccine, and priming with the attenuated salmonella vaccine and boosting with the recombinant adenovirus vaccine. Humoral and cellular immune responses were detected and evaluated. We then challenged the ducks with TMUV at 12 days after boosting to assay for clinical symptoms, mortality, viral loads and histopathological lesions after these different strategies. Results: Compared with the homologous prime-boost strategies, the heterologous prime-boost regimen produced higher levels of neutralizing antibodies and IgG antibodies against TMUV. Additionally, it could induce higher levels of IFN-γ than homologous prime-boost strategies in the later stage. Interestingly, the heterologous prime-boost strategy induced higher levels of IL-4 in the early stage, but the IL-4 levels gradually decreased and were even lower than those induced by the homologous prime-boost strategy in the later stage. Moreover, the heterologous prime-boost strategy could efficiently protect ducks, with low viral titres, no clinical symptoms and histopathological lesions in this experiment after challenge with TMUV, while slight clinical symptoms and histopathological lesions were observed with the homologous prime-boost strategies.

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Section: Virus and Vaccinesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Heterologous prime-boost: an important candidate immunization strategy against Tembusu virus

Pan

Jia

et al. 2020

Virol J

Self Cite

View full text Add to dashboard Cite

show abstract

“…The virus was propagated in the allantoic cavities of 10-day-old specific pathogen-free (SPF) embryonated duck eggs. Vaccine strain using attenuated salmonellapresented TMUV prM-E (pVAX-SME) was kindly gifted by Huang [20]. (The attenuated Salmonella typhimurium aroAstain SL7207 (S. typhimurium 2337-65 derivative hisG46 DEL407 [aroA::Tn10 (Tcs)]) used in this attenuated salmonella vaccine was kindly provided by Professor Kai Schulze of the Helmholtz Center for Infection Research (Germany).…”

Section: Virus and Vaccinesmentioning

confidence: 99%

Heterologous prime-boost: an important candidate immunization strategy against Tembusu virus

Pan

Jia²,

et al. 2020

Preprint

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Background Tembusu virus (TMUV), a newly emerging pathogenic flavivirus, spreads rapidly between ducks, causing massive economic losses in the Chinese duck industry. Vaccination is the most effective method to prevent TMUV. Therefore, it is urgent to look for an effective vaccine strategy against TMUV. Heterologous prime-boost regimens priming with vaccines and boosting with recombinant adenovirus vaccines have been proven to be successful strategies for protecting against viruses in experimental animal models. Methods In this study, heterologous and homologous prime-boost strategies using an attenuated vaccine and a recombinant adenovirus vaccine expressing prM-E or the E gene of TMUV were evaluated to protect ducks against TMUV infection for the first time, including priming and boosting with the attenuated salmonella vaccine, priming and boosting with the recombinant adenovirus vaccine, and priming with attenuated salmonella vaccine and boosting with the recombinant adenovirus vaccine. Humoral and cellular immune responses were detected and evaluated. We then challenged the ducks with TMUV at 12 days after boosting to assay for clinical symptoms, mortality, viral loads and histopathological lesions after these different strategies. Results Compared with the homologous prime-boost strategies, the heterologous prime-boost regimen produced higher levels of neutralizing antibodies and IgG antibodies against TMUV. Additionally, it could induce higher levels of IFN-γ than homologous prime-boost strategies in the later stage. Interestingly, the heterologous prime-boost strategy induced higher levels of IL-4 in the early stage, but the IL-4 levels gradually decreased and were even lower than those induced by the homologous prime-boost strategy in the later stage. Moreover, the heterologous prime-boost strategy could efficiently protect ducks, with low viral titres, no clinical symptoms and histopathological lesions in this experiment after challenge with TMUV, while slight clinical symptoms and histopathological lesions were observed with the homologous prime-boost strategies. Conclusions Our results indicated that the heterologous prime-boosts strategy induced higher levels of humoral and cellular immune responses and better protection against the TMUV infection in ducks than the homologous prime-boost strategies, suggesting that the heterologous prime-boost strategy is an important candidate for the design of a novel vaccine strategy against TMUV.

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“…Ducks orally vaccinated with this DNA vaccine were protected from lethal DTMUV infection. Oral administration of the DTMUV vaccine provided a fast vaccine delivery strategy and was economical for large-scale clinical applications [68]. Jiao et al reported that a DNA vaccine encoding the S1 and N genes delivered by S. enterica serovar Typhimurium via the oral and IN routes could induce humoral and mucosal immune responses and conferred 70% protection against IBV in chickens [25].…”

Section: Biological Carriersmentioning

confidence: 99%

Recent advances in delivery of veterinary DNA vaccines against avian pathogens

Jazayeri

Poh

2019

Vet Res

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Veterinary vaccines need to have desired characteristics, such as being effective, inexpensive, easy to administer, suitable for mass vaccination and stable under field conditions. DNA vaccines have been proposed as potential solutions for poultry diseases since they are subunit vaccines with no risk of infection or reversion to virulence. DNA vaccines can be utilized for simultaneous immunizations against multiple pathogens and are relatively easy to design and inexpensive to manufacture and store. Administration of DNA vaccines has been shown to stimulate immune responses and provide protection from challenges in different animal models. Although DNA vaccines offer advantages, setbacks including the inability to induce strong immunity, and the fact that they are not currently applicable for mass vaccination impede the use of DNA vaccines in the poultry industry. The use of either biological or physical carriers has been proposed as a solution to overcome the current delivery limitations of DNA vaccines for veterinary applications. This review presents an overview of the recent development of carriers for delivery of veterinary DNA vaccines against avian pathogens.

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Oral Delivery of a DNA Vaccine Expressing the PrM and E Genes: A Promising Vaccine Strategy against Flavivirus in Ducks

Cited by 18 publications

References 59 publications

Heterologous prime-boost: an important candidate immunization strategy against Tembusu virus

Heterologous prime-boost: an important candidate immunization strategy against Tembusu virus

Heterologous prime-boost: an important candidate immunization strategy against Tembusu virus

Recent advances in delivery of veterinary DNA vaccines against avian pathogens

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